Protein Kinase Protocols / Edition 1

Protein Kinase Protocols / Edition 1

by Alastair D. Reith
ISBN-10:
0896037002
ISBN-13:
9780896037007
Pub. Date:
09/28/2000
Publisher:
Springer-Verlag New York, LLC
ISBN-10:
0896037002
ISBN-13:
9780896037007
Pub. Date:
09/28/2000
Publisher:
Springer-Verlag New York, LLC
Protein Kinase Protocols / Edition 1

Protein Kinase Protocols / Edition 1

by Alastair D. Reith

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Overview

As key components of many cell signaling pathways, protein kinases are implicated in a broad variety of diseases, including cancers and neurodegenerative conditions, and offer considerable potential as tractable targets for therapeutic intervention. In Protein Kinase Prools, a panel of highly skilled laboratory investigators describe both basic and more sophisticated methods for the analysis of kinase-mediated signaling cascades, with emphasis on the identification of proteins according to their interactive relationships and the analysis of their functional properties. Described in step-by-step detail, these readily reproducible techniques offer novices quick access to a complicated field, and provide more experienced investigators many novel time-saving ploys. Emphasis is given to the critical technical steps that are often omitted from methods published in the primary literature. There are also tips on potential pitfalls and copious notes on how to adjust the prools to work in related systems.

Broad in its range of techniques and thoroughly detailed to help ensure experimental success, Protein Kinase Prools offers both novice and experienced investigators powerful tools for understanding the functional roles of specific protein kinases within signaling cascades and for identification and evaluation of novel therapeutic targets.


Product Details

ISBN-13: 9780896037007
Publisher: Springer-Verlag New York, LLC
Publication date: 09/28/2000
Series: Methods in Molecular Biology , #124
Edition description: 2001
Pages: 365
Product dimensions: 5.98(w) x 9.02(h) x 0.04(d)

About the Author

Reith, Alastair D. (GlaxoSmithKine, Essex, UK)

Table of Contents

Protein Kinase-Mediated Signaling Networks: Regulation and Functional Characterization, Alastair D. Reith. Cloning Protein Tyrosine Kinases by Screening cDNA Libraries with Antiphosphotyrosine Antibodies, Lisa D. Chong and Ira O. Daar. Immunoprecipitation and Western Blotting of Phosphotyrosine-Containing Proteins, Kathleen M. Woods-Ignatoski. Two-Dimensional Phosphoamino Acid Analysis, Peter Blume-Jensen and Tony Hunter. Two-Dimensional Phosphopeptide Mapping of Receptor Tyrosine Kinases, Jeremy M. Tavaré and Tarik Issad. Identification of the Sites of Phosphorylation in Proteins Using High Performance Liquid Chromatography and Mass Spectrometry, A. Grey Craig. Phosphorylation of Smad Signaling Proteins by Receptor Serine/ Threonine Kinases, Serhiy Souchelnytskyi, Lars Rönnstrand, Carl-Henrik Heldin, and Peter ten Dijke. Assays for Mitogen-Activated Protein Kinase (MAPK) Subtypes and MAPK Activating Protein Kinase-2 (MAPKAP K-2) Using a Common Cell Lysate, Rozen Le Panse, Navita Rampersaud, and Louis C. Mahadevan. JAK-Mediated Phosphorylation and Activation of STAT Signaling Proteins: Analysis by Phosphotyrosine Blotting and EMSA, Nicola Broughton and Mark S. Burfoot. Assays for Glycogen Synthase Kinase-3 (GSK-3), Darren Cross. Cyclin-Dependent Kinases and Cyclin-Dependent Kinase Inhibitors: Detection Methods and Activity Measurements, Gavin Brooks. Protein Histidine Kinase, Harry R. Matthews and Karina Chan. Cloning and Expression of Recombinant HEXA-HIS Tagged ZAP-70 Using the Baculovirus System, Tracey Purton, Sandra Wilkinson, and Edward J. Murray. Analysis of Protein Kinase Subcellular Localization by Visualization of GFP Fusion Proteins, Ralph A. Zirngibl and Peter Greer. Detection of Phosphorylation-Dependent Interactions by Far-Western Gel Overlay, Mary Rose Burnham, Regina DeBerry, and Amy H. Bouton. Purification of Tyrosine-Phosphorylated Proteins by Immunoaffinity Chromatography and Direct Cloning of Their cDNAs from Bacterial Expression Libraries,Pier Paolo Di Fiore and Francesca Fazioli. Identification of Receptor Tyrosine Kinase (RTK) Substrates by the Cloning of Receptor Targets (CORT) Strategy, Roger J. Daly. Identification of Receptor Tyrosine Kinase Associating Proteins Using the Yeast Two-Hybrid System, Sally A. Prigent. cDNA Expression Cloning and Charaterization of Phosphorylation Dependent Protein Interactors Using the Yeast Tribrid System, Christoph Volpers, Manuel Lubinus, Mark A. Osborne, and Jarema P. Kochan. Analysis of SH2 Domain-Phosphopeptide Interactions by Isothermal Titration Calorimetry and Surface Plasmon Resonance, George Panayotou and John Ladbury. Cloning and Characterization of RTK Ligands Using Receptor-Alkaline Phosphatase Fusion Proteins, Hwai-Jong Cheng and John G. Flanagan. Isolation and Characterization of 'Orphan-RTK' Ligands Using an Integrated Biosensor Approach, Martin Lackmann. Index.
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