Pub. Date:
Cold Spring Harbor Laboratory Press
Protein-Protein Interactions: A Molecular Cloning Manual

Protein-Protein Interactions: A Molecular Cloning Manual

by Erica A. Golemis
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As more genetic and biochemical information about the protein components of cells accumulate, the analysis of protein-protein interactions is becoming increasingly important. This manual presents a wide range of techniques for identifying and analyzing these interactions, starting with standard molecular and biochemical techniques, and progressing to biophysical and computational approaches and therapeutic and other post-genomic applications. This manual is designed to complement the information in the best-selling Molecular Cloning manual, and is presented in the same clear, user-friendly format. It is an essential resource for investigators studying interacting sets of proteins and their physiologic significance in a wide range of experimental systems.

Product Details

ISBN-13: 9780879696283
Publisher: Cold Spring Harbor Laboratory Press
Publication date: 12/17/2001
Pages: 672
Product dimensions: 8.66(w) x 11.00(h) x 1.15(d)

Table of Contents

Chapter 1: Toward an Understanding of Protein Interactions

Section One: The Biological Context for Protein Interaction Studies: Physiological Indicators of Protein-Protein Interaction
Chapter 2: Signal Transduction and Mammalian Cell Growth: Problems and Paradigms
Chapter 3: Impact of Protein Interaction Technologies on Cancer Biology and Pharmacogenomics

Section Two: Standard Technologies Used to Identify and Characterize Protein-Protein Interactions
Chapter 4: Identification of Protein-Protein Interactions with Glutathione-S-Transferase Fusion Proteins
Chapter 5: Identification of Associated Proteins by Coimmunoprecipitation
Chapter 6: Chemical Cross-linking in Studying Protein-Protein Interactions
Chapter 7: Yeast and Bacterial Two-hybrid Selection Systems for Studying Protein-Protein Interactions
Chapter 8: Phage-display Approaches for the Study of Protein-Protein Interactions
Chapter 9: Genetic Approaches to Identify Protein-Protein Interactions in Budding Yeast

Section Three: Biophysical Approaches to Identify and Characterize Protein-Protein Interactions
Chapter 10: Imaging Protein Interactions by FRET Microscopy
Chapter 11: Detection of Homotypic Protein Interactions with Green Fluorescent Protein Proximity Imaging (GFP-PRIM)
Chapter 12: Characterization of Multiprotein Complexes by Mass Spectrometry
Chapter 13: Probing Ligand-Receptor Interactions with Atomic Force Microscopy
Chapter 14: Analysis of Interacting Proteins with Surface Plasmon Resonance Using Biacore
Chapter 15: Analysis of Protein Interactions Using a Quartz Crystal Microbalance Biosensor

Section Four: Recent Developments and Other Tools: Reviews and Short Protocols
Chapter 16: Protease Footprinting
Chapter 17: Tandem Affinity Purification to Enhance Interacting Protein Identification
Chapter 18: Protein Purification by Inverse Transition Cycling
Chapter 19: Using In Vitro Expression Cloning to Identify Interacting Proteins
Chapter 20: Using EMXENOPUS Egg Extracts to Modify Recombinant Proteins
Chapter 21: Using lambda Repressor Fusions to Isolate and Characterize Self-assembling
Chapter 22: The Membrane-based Yeast Two-hybrid System
Chapter 23: Protein Interactions in Live Cells Monitored by beta-Galactosidase Complementation
Chapter 24: Identification of Protein Single-chain Antibody Interactions In Vivo Using Two-hybrid Protocols
Chapter 25: Protein Interactions and Library Screening with Protein Fragment Complementation Strategies
Chapter 26: A Bacterial Two-hybrid System Based on a Cyclic AMP Signaling Cascade
Chapter 27: Peptide Aptamers to Study Proteins and Protein Networks
Chapter 28: Generation of Protein Fragment Libraries by Incremental Truncation
Chapter 29: Catalytic Antibodies: New Characters in the Protein Repertoire
Chapter 30: In Vitro Selection and Evolution of Protein-Ligand Interactions by Ribosome Display
Chapter 31: Analysis of Protein Interactions with Peptides Synthesized on Membranes
Chapter 32: Protein Bundling to Enhance the Detection of Protein-Protein Interactions

Section Five: Protein-Protein Interactions: Computation and the Future
Chapter 33: Analysis of Genome-wide Protein Interactions Using Computational Approaches
Chapter 34: Visualization and Integration of Protein-Protein Interactions
Chapter 35: Modulating Protein-Protein Interactions to Develop New Therapeutic Approaches

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