Proteoglycans: Methods and Protocols

Proteoglycans: Methods and Protocols

by Françoise Rédini (Editor)


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Product Details

ISBN-13: 9781617794971
Publisher: Springer-Verlag New York, LLC
Publication date: 01/19/2012
Series: Methods in Molecular Biology , #836
Edition description: 2012
Pages: 359
Product dimensions: 7.01(w) x 10.00(h) x 0.04(d)

Table of Contents

Part I: Proteoglycans

1. Proteoglycans: Gene Cloning

Mauricio Cortes, James R. Mensch, Miriam Domowicz, and Nancy B. Schwartz

2. Proteoglycan: Site Mapping and Site-Directed Mutagenesis

Fred K. Hagen

3. Mapping of the Wnt/β-Catenin/TCF Response Elements in the Human Versican Promoter

Maziar Rahmani, Jon M. Carthy, and Bruce M. McManus

4. Gene Silencing in Mouse Embryonic Stem Cells

Norihiko Sasaki and Shoko Nishihara

5. A Novel Strategy for a Splice-Variant Selective Gene Ablation: The Example of the Versican V0/V2 Knockout

María T. Dours-Zimmermann and Dieter R. Zimmermann

6. Detection of Neurocan in Cerebrospinal Fluid

Uwe Rauch

Part II: Glycosaminoglycans Analysis

7. Glycosaminoglycan Chain Analysis and Characterization (Glycosylation/Epimerization)

Shuji Mizumoto and Kazuyuki Sugahara

8. Characterization of Glycosaminoglycans by Tandem Vibrational Microspectroscopy and Multivariate Data Analysis

Nathalie Mainreck, Stéphane Brézillon, Ganesh D. Sockalingum, François-Xavier Maquart, Michel Manfait, and Yanusz Wegrowski

9. Glycosaminoglycans: Oligosaccharide Analysis by Liquid Chromatography/Specific Labeling

Derek J. Langelsay, Christopher J. Jones, Szabolcs Beni, and Cynthia K. Larive

10. Brain Chondroitin/Dermatan Sulfate, from Cerebral Tissue to Fine Structure: Extraction, Preparation, and Fully Automated Chip-Electrospray Mass Spectrometric Analysis

Alina D. Zamfir, Corina Flangea, Alina Serb, Eugen Sisu, Leon Zagrean, Andreas Rizzi, and Daniela G. Seidler

11. Use of Neutrons Reveal the Dynamics of Cell Surface Glycosaminoglycans

Marion Jasnin

12. Following Protein-Glycosaminoglycan (GAG) Polysaccharide Interactions with Differential Scanning Fluorimetry (DSF)

Katarzyna A. Uniewicz, Alessandro Ori, Timothy R. Rudd, Marco Guerrini, Mark C. Wilkinson, David G. Fernig, and Edwin A. Yates

13. In Vivo Scintigraphic Imaging of Proteoglycans

Elisabeth Miot-Noirault, Aurélien Vidal, Philippe Auzeloux, Caroline Peyrode, Jean-Claude Madelmont, and Jean-Michel Chezal

Part III: PGs: Multifunctional Cell Regulators

14. Serglycin: The Master of the Mast Cell

Elin Rönnberg and Gunnar Pejler

15. Analysis of Aggrecan Catabolism by Immunoblotting and Immunohistochemistry

Peter J. Roughley and John S. Mort

16. Heparan Sulfate Proteoglycans as Multifunctional Cell Regulators: Cell Surface Receptors

Jin-ping Li and Dorothe Spillmann

Part IV: Proteoglycans Involvement in Pathophysiology

17. Models for Studies of Proteoglycans in Kidney Pathophysiology

Scott J. Harvey

18. Lumican Promotes Corneal Epithelial Wound Healing

Chia-Yang Liu and Winston Whei-Yang Kao

19. Shedding of Cell Membrane-Bound Proteoglycans

Eon Jeong Nam and Pyong Woo Park

20. Modulatory Effects of Proteoglycans on Proteinase Activities

Steven Georges, Dominique Heymann, and Marc Padrines

21. Proteoglycans and Osteolysis

Marc Baud’Huin, Céline Charrier, Gwenola Bougras, Régis Brion, Frédéric Lezot, Marc Padrines, and Dominique Heymann

22. Proteoglycans and Cartilage Repair

Mohamed Ouzzine, Narayanan Venkatesan, and Sylvie Fournel-Gigleux

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