Protocols in Human Molecular Genetics

Protocols in Human Molecular Genetics

by Christopher G. Mathew



Product Details

ISBN-13: 9780896032057
Publisher: Springer-Verlag New York, LLC
Publication date: 01/28/1991
Series: Methods in Molecular Biology Series , #9
Pages: 472
Product dimensions: 0.00(w) x 0.00(h) x (d)

Table of Contents

The Polymerase Chain Reaction: Getting Started. Direct DNA Sequencing of Complementary DNA Amplified by the Polymerase Chain Reaction. Direct-Sequencing of PCR-Amplified DNA. Rapid DNA Sequence Analysis Using Fluorescent Labels. Detection of Mutations in DNA and RNA by Chemical Cleavage. Rapid Methods for Detection of Polymorphic Markers in Genomic DNA. The Analysis of Point Mutations Using Synthetic Oligonucleotide Probes. Detection of Mutations by the Amplification Refractory Mutation System (ARMS). Automated Gene Detection Using the Oligonucleotide Ligation Assay. Detection of Point Mutations by Denaturing-Gradient Gel Electrophoresis. The Detection and Mapping of Point Mutations by RNase A Cleavage. Discontinuous Polyacrylamide Gel Electrophoresis of DNA Fragments. Extraction and Enzymatic Amplification of DNA from Paraffin-Embedded Specimens. The Use of the Polymerase Chain Reaction in the Mapping of Human Genes Using Somatic Cell Hybrids. The Southern Blot: An Update. The Detection of Specific DNA Sequences by Enhanced Chemiluminescence. Pulsed-Field Gel Electrophoresis. Cloning from Gels Following Pulse-Field Gel Electrophoresis. Yeast Artificial-Chromosome (YAC) Cloning Systems. Gene Targeting for Somatic Cell Manipulation. In Situ Hybridization of Chromosomes. DNA Fingerprinting Analysis: Methodology and Its Applications. DNA Fingerprinting and Forensic Medicine. The Detection of Point Mutations in Hemoglobin Defects Using Allele-Specific Oligonucleotide Probes. Detection of Gene Deletions Using Multiplex Polymerase Chain Reactions. Application of Pulsed-Field Gel Electrophoresis to Genetic Diagnosis. Molecular Diagnostics of Cancer. The Detection of Latent Virus Infection by Polymerase Chain Reaction. Mapping Inherited Diseases by Linkage Analysis. Diagnosis of Genetic Disorders with Linked DNA Markers. Software for Genetic Linkage Analysis. Creating Animal Models of Genetic Diseases. Molecular Biology and Medicine: Ethical Implications. Appendix. Index.

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