RNA: Protein Interactions / Edition 1

RNA: Protein Interactions / Edition 1

by Christopher W. J. Smith
ISBN-10:
0199636508
ISBN-13:
9780199636501
Pub. Date:
07/28/1998
Publisher:
Oxford University Press, USA

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Overview

RNA: Protein Interactions / Edition 1

RNA-Protein Interactions: A Practical Approach provides a clear and comprehensive guide to the many experimental procedures used in studying RNA-protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction to various biochemical and genetic approaches, and from in-depth methods for analyzing the structural basis of the interaction to methods for purifying and cloning RNA binding proteins. The volume contains a number of procedures that have not previously been covered in this type of manual, including the production of site-specifically modified RNAs by enzymatic and chemical methods and in vivo screening for novel RNA-protein interactions in yeast and E. coli. This informative book will be a valuable aid to laboratory workers in biochemistry and molecular biology, as well as graduate students and postdoctoral and senior scientists, whose research encompasses this field.

Product Details

ISBN-13: 9780199636501
Publisher: Oxford University Press, USA
Publication date: 07/28/1998
Series: Practical Approach Series , #192
Edition description: New Edition
Pages: 368
Product dimensions: 6.10(w) x 9.20(h) x 0.80(d)

Table of Contents

1. Applications of chemically synthesized RNA
2. Preparation of RNA-Protein complexes for X-ray crystallography and NMR
3. Uses of site-specifically modified RNAs constructed by RNA ligation
4. The Electrophoretic Mobility Shift Assay for RNA Binding Proteins
5. Affinity methods for isolating RNA-binding proteins
6. Synthetic lethal/enhancer screening to identify snRNA-protein and protein-protein interactions in yeast pre-mRNA splicing
7. Detecting RNA-Protein Interactions and Isolating cDNA Clones by Northwestern Screening
8. A three-hybrid system to detect and analyse RNA-protein interactions in vivo
9. In vivo selection of specific RNA-binding polypeptides using a transcription anti-termination reporter assay
10. Foot-printing and modification-interference analysis of binding sites on RNA
11. Analysis of RNA-Protein and RNA-RNA contacts between ribosomes and functional ligands using site-directed cross-linking techniques
12. In vitro selection of nucleic acid ligands

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