The present conference is the third in a series on this topic sponsored by the NCP. Drs. HcGhee, l1estecky, Genco and Bowen are to be commended for arranging this truly comprehensive program. We are fortunate that they have been able to assemble such a wealth of expertise. Program staff considers the advice of scientists such as yourselves essential to the success of its mission. Your presentations and discussions will focus on the crucial problems to be solved in exploiting the secretory immune system to combat dental caries. The published proceedings will bring these to the attention of the research community quickly and hopefully they will stimulate new investigators to bring their talents to these problems. This meeting will, to a large extent, determine the direction of research sponsored by the NCP. Finally, I would like to thank the members of the planning committee for their dedicated efforts over the past two years, which have culminated in this symposium. Our thanks are also due to each of you, in advance, for contributing so freely to the success of this meeting.
Table of ContentsIntroductory Address.- New Areas Arising from Studies of Secretory Immunity.- Session I: Cellular Aspects of Mucosal Immunity.- Generation of Clonal and Isotype Diversity.- Cellular Parameters of the IgA Response.- Lymphatic Physiology and Secretory Immunity.- Development of the IgA System in the Mammary Gland.- Effect of Anti-IgD Serum on Immune Responses.- A Common Mucosal Immunologic System Involving the Bronchus, Breast and Bowel.- Discussion I.- Migration Pathways of T Lymphocytes in the Small Intestine.- Cell-Mediated Immunity on Secretory Surfaces.- Immunoglobulin A Production by Human Colostral Cells: Quantitative Aspects.- Immunologic Aspects of Human Colostrum and Milk: Interaction with the Intestinal Immunity of the Neonate.- Discussion II.- Session II: Induction of a Secretory Immune Response.- Remote-Site Stimulation of Secretory IgA Antibodies Following Bronchial and Gastric Stimulation.- Development of Humoral Antibody Following the Ingestion of Soluble Protein Antigen by Passively Immunized Animals.- Elimination of Escherichia coli K88 Adhesion Determinant by Antibody in Porcine Gut and Mammary Secretions Following Oral Immunization.- Cellular Aspects of the Murine Anti-Hapten IgA Response.- Discussion I.- Characterization of Immunoglobulin-Containing Cells in the Submandibular Gland of the Rat After Local Immunization.- Secretory IgA Antibodies to Enterobacterial Virulence Antigens: Their Induction and Possible Relevance.- Induction of Secretory Antibodies in Humans Following Ingestion of Streptococcus mutans.- Concept of the Local and Common Mucosal Immune Response.- Discussion II.- Session III: Biosynthesis and Transport of Secretory Antibodies.- T-Cell Regulation of Murine IgA Biosynthesis.- Molecular Cellular Interactions in the Secretory IgA System.- Further Evidence for a Role of Secretory Component (SC) and J Chain in the Glandular Transport of IgA.- Influence of Parenteral Immunization in Rabbits on the Penetrability of Oral Mucosa for Macromolecules.- Role of the Liver in the Rat Intestinal s-IgA System.- Transport of Oligomeric IgA of Systemic Origin Into External Secretions.- Discussion I.- Session IV: Caries Immunity.- A. Animal Studies.- Effective Immunity to Dental Caries: Selective Induction of Secretory Immunity by Oral Administration of Streptococcus mutans in Rodents.- Cross-Protective Aspects of Glucosyltransferase Antigens in the Hamster Caries Model.- Antigens of Streptococcus mutans Implicated in Virulence-Production of Antibodies.- The Effect of Cyclosporin A on Dental Caries in Rats Monoassoeiated with Actinomyces viscosus NY 1.- Passive Immunization with Antisera to Streptococcus mutans in the Prevention of Caries in Rhesus Monkeys.- Antibody Binding of Glucosyltransferase Enzyme Preparations from Homologous and Heterologous Serotypes of S. mutans.- Daucus carrota (Carrot) A Selective Bacteriosorbent.- Discussion I.- B. Human Studies.- Studies of Secretory IgA in Caries-Resistant and Caries-Susceptible Adults.- Antibodies Reacting with Streptococcus mutans in Secretions from Minor Salivary Glands in Humans.- Salivary IgA Antibodies to Antigens from Streptococcus mutans in Human Dental Caries.- Antibodies Against Streptococcus mutans and Glucosyltransferases in Caries-Free and Caries-Active Military Recruits.- Immunoglobulins and Antibodies in Plaque Fluid and Saliva in Two Populations with Contrasting Levels of Caries.- Discussion I.- Secretory Immunity and Immunodeficiency.- The Partial Isolation and Function of Salivary Factors Which Interact with the Complement System: A Possible Role in Mucosal Immunity.- Discussion II.- Session V: Studies of Virulence and Immunity.- Adhesion and Chemotaxis as Determinants of Bacterial Association with Mucosal Surfaces.- The Role of Antigen Form and the Induction of an Intestinal Immune Response to Cholera Toxin/Toxoid in Rats.- Motility of the Pathogen and Intestinal Immunity of the Host in Experimental Cholera.- Discussion I.- Prevention of Bacterial Infections of Mucosal Surfaces by Immune Secretory IgA.- Studies on Oral Adjuvants Against Murine Salmonellosis.- Cell Mediated Immune Responses to Bacterial Antigens on Human Mucosal Surfaces.- Assay and Properties of IgA Protease of Streptococcus sanguis.- Discussion II.- Session VI A: Structural and Functional Studies of Secretory Immunoglobulins.- A. Structure.- A Comparison of Secretory Component Immunoglobulin Interactions Amongst Different Species.- Serologic and Structural Comparisons of Rabbit IgA Allotypes.- Milk Precipitins, Circulating Immune Complexes and IgA Deficiency.- Regulation by Sex Hormones of Immunoglobulins in Rat Uterine and Vaginal Secretions.- Discussion.- B. Functional Aspects.- Effect on Rat IgA Synthesis by Isotypic Suppression with an Anti-Rat Delta Heavy Chain Serum.- Immunoglobulin A in Secretions from the Lower Human Respiratory Tract.- Measurement of IgA Memory to 2,4-Dinitrophenyl (DNP) in the Sera of Adoptive Recipient Rats Using the Amplified Elisa.- Human Mucosal Lymphocytes Memory for ‘Recall’ Antigens and Non-Specific Suppression by T-Lymphocytes.- Response of Anamnestic IgA-Producing Cells in the Mouse Gut After Repeated Intragastric Immunization.- Detection of IgA Anti-Escherichia Coli Plasma Cells in the Intestine and Salivary Glands of Pigs Orally and Locally Infected with E. Coli.- Discussion.- Session VI B: Antigens of Oral Bacteria.- A. Lipoteichoic Acid and Other Antigens.- Lipoteichoic Acid The Key to the Adhesiveness of Sucrose Grown Streptococcus Mutans.- Amphipathic Antigens of Oral Microorganisms Immunogenicity and Other Biological Properties.- Effect of Growth Conditions on the Antigenic Components of Streptococcus mutans and Lactobacilli.- Modification of In Vitro Adherence of Streptococcus mutans by Plant Lectins.- Search for Cross-Reacting Antigens of Oral Acidogenic Bacteria and Members of the Normal Intestinal Flora.- Discussion I.- Genetic Alterations of Streptococcus mutans’ Virulence.- Use of Mutants in the Elucidation of Virulence of Streptococcus mutans.- Wall Carbohydrate Antigens of A. Israelii.- Fimbriae of Actinomyces viscosus T14V: Their Relationship to the Virulence-Associated Antigen and to Coaggregation with Streptococcus sanguis 34.- Preliminary Results in the Immunization of Irus Monkeys Against Dental Caries.- Discussion II.- Session VII: Antigens of Oral Bacteria.- B. Studies with Glucosyltransferases.- Streptococcus mutans Dextransucrase: Phosphoglycerides and the Detection of Inhibitory Antibodies in Sera.- Interaction of Streptococcus mutans Glucosyltransferases with Antibodies.- Dextran/Glucan Binding by Streptococcus mutans: The Role of Molecular Size and Binding Site in Agglutination.- Multiple Forms of Dextran-Binding Proteins from Streptococcus mutans.- Discussion.- General Discussion.- Session VII: Antigens of Oral Bacteria Chairman: Arnold S. Bleiweis.- C. Surface Glucans.- Dextran Receptors as Immunogens in Caries Prophylaxis.- Antigenicity and Immunogenicity of Cell-Associated Glucans from Streptococcus mutans.- A Comparison of the Phenol Water and Rantz and Randall Teichoic Acid Antigens in Group H Streptococci.- Turnover and Excretion of Streptococcal Surface Components.- Discussion.- Session VIII: Ecological Considerations.- Fluorescence Quantitation of S-IgA in Parotid Saliva and of S-IgA Bound to Oral Microorganisms.- Antigenic Variation in Populations of Oral Streptococci.- Salivary Immunoglobulins in Diseases Affecting Salivary Glands.- Whole Saliva Proteases: Development of Methods for Determination of Origins.- Extrachromosomal Gene Systems in Streptococcus mutans.- Bacterial Adherence Related to Agglutination and Surface Film Formation.- Discussion.- Report of the Summation Committee: Recommended Areas for Future Research.