Single-Channel Recording

by Bert Sakmann (Editor), Erwin Neher (Editor)

Overview

Edited by the 1991 winners of the Nobel Prize in Physiology or Medicine, this Second Edition includes new chapters covering such applications as capacitance measurements; single-cell PCR measurements; whole-cell recording from brain slices in combination with imaging techniques; atomic force microscopy of cells and membranes attached to glass pipettes; and patch clamping.

Product Details

ISBN-13:	9781441912305
Publisher:	Springer US
Publication date:	12/09/2009
Edition description:	Second Edition 1995
Pages:	700
Product dimensions:	7.10(w) x 10.00(h) x 1.80(d)

Table of Contents

to Patch Clamping.- A Practical Guide to Patch Clamping.- Tight-Seal Whole-Cell Recording.- Guide to Data Acquisition and Analysis.- Methods.- Electronic Design of the Patch Clamp.- Low-Noise Recording.- Voltage Offsets in Patch-Clamp Experiments.- Techniques for Membrane Capacitance Measurements.- Patch-Pipette Recordings from the Soma, Dendrites, and Axon of Neurons in Brain Slices.- Patch Clamp and Calcium Imaging in Brain Slices.- Fast Application of Agonists to Isolated Membrane Patches.- Electrochemical Detection of Secretion from Single Cells.- Technical Approaches to Studying Specific Properties of Ion Channels in Plants.- The Giant Membrane Patch.- A Fast Pressure-Clamp Technique for Studying Mechanogated Channels.- Electrophysiological Recordings from Xenopus Oocytes.- Polymerase Chain Reaction Analysis of Ion Channel Expression in Single Neurons of Brain Slices.- Force Microscopy on Membrane Patches.- Analysis.- The Principles of the Shastic Interpretation of Ion-Channel Mechanisms.- Fitting and Statistical Analysis of Single-Channel Records.- A Q-Matrix Cookbook.- “Classics”.- Geometric Parameters of Pipettes and Membrane Patches.- Conformational Transitions of Ionic Channels.

Editorial Reviews

The single-channel recording technique has reached the status of a routine method, and the view that conductance changes in biological membranes are caused by the openings and closings of ion channels is now almost universally accepted. This volume contains 22 chapters in four parts: introduction to patch clamping; methods; analysis; and "classics." There is also an appendix on improved patch-clamp techniques for high-resolution current recording from cells and cell- free membrane patches. The first edition was published in 1983. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

From reviews of the First Edition:

"A landmark in the field of membrane biophysics." (Science)

"A first-class laboratory guide...an indispensable reference." (Neuroscience)

From the Publisher