Stem Cell Heterogeneity: Methods and Protocols

Stem Cell Heterogeneity: Methods and Protocols

Paperback(Softcover reprint of the original 1st ed. 2016)

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Stem Cell Heterogeneity: Methods and Protocols by Kursad Turksen

Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity. This goes against the long-held tenet that stem cells, defined by their capacity for self-renewal and lineage development, comprised a homogenous population, thus providing the reader with a new avenue of exploration into the complex world of stem cell study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Stem Cell Heterogeneity: Methods and Protocols serves as an ideal guide for investigators exploring this important area of research.

Product Details

ISBN-13: 9781493982349
Publisher: Springer New York
Publication date: 06/20/2018
Series: Methods in Molecular Biology , #1516
Edition description: Softcover reprint of the original 1st ed. 2016
Pages: 390
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

1. The Heterogeneity of Stem Cells: A Brief Overview

Györgyi Műzes and Ferenc Sipos

2. Establishment and Characterization of Naïve Pluripotency in Human Embryonic Stem Cells

Sharat Warrier, Mina Popovic, Margot Van der Jeught, and Björn Heindryckx

3. An Effective and Reliable Xeno-Free Cryopreservation Protocol for Single Human Pluripotent Stem Cells

Guoliang Meng, Anna Poon, Shiying Liu, and Derrick E. Rancourt

4. Clonal Analysis of Cells with Cellular Barcoding: When Numbers and Sizes Matter

Leonid V. Bystrykh and Mirjam E. Belderbos

5. Analysis of Cell Cycle Status of Murine Hematopoietic Stem Cells

Krzysztof Szade, Karolina Bukowska-Strakova, Monika Zukowska, Alicja Jozkowicz, and Józef Dulak

6. Dissecting Transcriptional Heterogeneity in Pluripotency: Single Cell Analysis of Mouse Embryonic Stem Cells

Ana M.V. Guedes, Domingos Henrique, and Elsa Abranches

7. Generation of Regionally Specific Neural Progenitor Cells (NPCs) and Neurons from Human Pluripotent Stem Cells (hPSCs)

Josh Cutts, Nicholas Brookhouser, and David. A Brafman

8. Isolation and Culture of Embryonic Stem Cells, Mesenchymal Stem Cells, and Dendritic Cells from Humans and Mice

Srabani Kar, Shinjini Mitra, and Ena Ray Banerjee

9. Decoding the Epigenetic Heterogeneity of Human Pluripotent Stem Cells with Seamless Gene Editing

Amar M. Singh, Dustin W. Perry, Valeriya V. Adjan Steffey, Kenneth Miller, and Daniel W. Allison

10. Stencil Micropatterning for Spatial Control of Human Pluripotent Stem Cell Fate Heterogeneity

Jun Yuan, Geetika Sahni, and Yi-Chin Toh

11. Visualizing the Functional Heterogeneity of Muscle Stem Cells

Yasuo Kitajima, Shizuka Ogawa, and Yusuke Ono

12. Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies

Chiara Franzin, Martina Piccoli, Luca Urbani, Carlo Biz, Piergiorgio Gamba, Paolo De Coppi, and Michela Pozzobon

13. Measuring ATP Concentration in a Small Number of Murine Hematopoietic Stem Cells

Krzysztof Szade, Monika Zukowska, Alicja Jozkowicz, and Józef Dulak

14. Growth Factor-Free Pre-Vascularization of Cell Sheets for Tissue Engineering

Marina Costa, Rogério P. Pirraco, Mariana T. Cerqueira, Rui L. Reis, and Alexandra P. Marques

15. In Vitro Culture of Human Hematopoietic Stem Cells in Serum Free Medium and Their Monitoring by Flow Cytometry

Marc Cloutier, Christine Jobin, Carl Simard, and Sonia Néron

16. Aerosol-Based Cell Therapy for Treatment of Lung Diseases

Badrul Hisham Yahaya, Egi Kardia, and Nur Shuhaidatul Sarmiza Abdul Halim

17. Induction of Inner Ear Hair Cells from Mouse Embryonic Stem Cells In Vitro

Masahide Yoshikawa and Yukiteru Ouji

18. Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro

Yukiteru Ouji and Masahide Yoshikawa

19. Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro

Yukiteru Ouji and Masahide Yoshikawa

20. Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method

Ching-Hui Lin, Hao-Chen Chang, and Chia-Hsien Hsu

21. Automated Cell-Based Quantitation of 8-OHdG Damage

Bilge Debelec-Butuner, Aykut Bostancı, Lisa Heiserich, Caroline Eberle, Filiz Ozcan, Mutay Aslan, Dirk Roggenbuck, and Kemal Sami Korkmaz

22. CoCl2 Administration to Vascular MSC Cultures as an In Vitro Hypoxic System to Study Stem Cell Survival and Angiogenesis

Carmen Ciavarella, Silvia Fittipaldi, and Gianandrea Pasquinelli

23. Reporter Systems to Study Cancer Stem Cells

Caner Saygin, Mohamed Samour, Anastasia Chumakova, Awad Jarrar, Justin D. Lathia, and Ofer Reizes

24. Agent-Based Modeling of Cancer Stem Cell Driven Solid Tumor Growth

Jan Poleszczuk, Paul Macklin, and Heiko Enderling

25. Induction of a Tumor-Metastasis-Receptive Microenvironment as an Unwanted Side Effect After Radio/Chemotherapy and In Vitro and In Vivo Assays to Study This Phenomenon

Gabriela Schneider, Zachariah Payne Sellers, and Mariusz Z. Ratajczak

26. Isolation and Propagation of Glioma Stem Cells from Acutely Resected Tumors

Jin-Kyu Jung, Mark R. Gilbert, and Deric M. Park

27. Isolation and Characterization of Cancer Stem Cells of the Non-Small Cell Lung Cancer (A549) Cell Line

Nazilah Abdul Satar, Norashikin Zakaria, Noor Hanis Abu Halim, and Badrul Hisham Yahaya

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