Stem Cell Heterogeneity: Methods and Protocols

Stem Cell Heterogeneity: Methods and Protocols

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Overview

Stem Cell Heterogeneity: Methods and Protocols by Kursad Turksen

Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity. This goes against the long-held tenet that stem cells, defined by their capacity for self-renewal and lineage development, comprised a homogenous population, thus providing the reader with a new avenue of exploration into the complex world of stem cell study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Stem Cell Heterogeneity: Methods and Protocols serves as an ideal guide for investigators exploring this important area of research.

Product Details

ISBN-13: 9781493982349
Publisher: Springer New York
Publication date: 06/20/2018
Series: Methods in Molecular Biology , #1516
Edition description: Softcover reprint of the original 1st ed. 2016
Pages: 390
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

1. The Heterogeneity of Stem Cells: A Brief Overview


Györgyi Műzes and Ferenc Sipos



2. Establishment and Characterization of Naïve Pluripotency in Human Embryonic Stem Cells


Sharat Warrier, Mina Popovic, Margot Van der Jeught, and Björn Heindryckx



3. An Effective and Reliable Xeno-Free Cryopreservation Protocol for Single Human Pluripotent Stem Cells



Guoliang Meng, Anna Poon, Shiying Liu, and Derrick E. Rancourt



4. Clonal Analysis of Cells with Cellular Barcoding: When Numbers and Sizes Matter


Leonid V. Bystrykh and Mirjam E. Belderbos



5. Analysis of Cell Cycle Status of Murine Hematopoietic Stem Cells


Krzysztof Szade, Karolina Bukowska-Strakova, Monika Zukowska, Alicja Jozkowicz, and Józef Dulak



6. Dissecting Transcriptional Heterogeneity in Pluripotency: Single Cell Analysis of Mouse Embryonic Stem Cells


Ana M.V. Guedes, Domingos Henrique, and Elsa Abranches



7. Generation of Regionally Specific Neural Progenitor Cells (NPCs) and Neurons from Human Pluripotent Stem Cells (hPSCs)


Josh Cutts, Nicholas Brookhouser, and David. A Brafman



8. Isolation and Culture of Embryonic Stem Cells, Mesenchymal Stem Cells, and Dendritic Cells from Humans and Mice



Srabani Kar, Shinjini Mitra, and Ena Ray Banerjee



9. Decoding the Epigenetic Heterogeneity of Human Pluripotent Stem Cells with Seamless Gene Editing


Amar M. Singh, Dustin W. Perry, Valeriya V. Adjan Steffey, Kenneth Miller, and Daniel W. Allison



10. Stencil Micropatterning for Spatial Control of Human Pluripotent Stem Cell Fate Heterogeneity


Jun Yuan, Geetika Sahni, and Yi-Chin Toh



11. Visualizing the Functional Heterogeneity of Muscle Stem Cells



Yasuo Kitajima, Shizuka Ogawa, and Yusuke Ono



12. Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies


Chiara Franzin, Martina Piccoli, Luca Urbani, Carlo Biz, Piergiorgio Gamba, Paolo De Coppi, and Michela Pozzobon



13. Measuring ATP Concentration in a Small Number of Murine Hematopoietic Stem Cells


Krzysztof Szade, Monika Zukowska, Alicja Jozkowicz, and Józef Dulak



14. Growth Factor-Free Pre-Vascularization of Cell Sheets for Tissue Engineering


Marina Costa, Rogério P. Pirraco, Mariana T. Cerqueira, Rui L. Reis, and Alexandra P. Marques



15. In Vitro Culture of Human Hematopoietic Stem Cells in Serum Free Medium and Their Monitoring by Flow Cytometry


Marc Cloutier, Christine Jobin, Carl Simard, and Sonia Néron



16. Aerosol-Based Cell Therapy for Treatment of Lung Diseases


Badrul Hisham Yahaya, Egi Kardia, and Nur Shuhaidatul Sarmiza Abdul Halim



17. Induction of Inner Ear Hair Cells from Mouse Embryonic Stem Cells In Vitro


Masahide Yoshikawa and Yukiteru Ouji



18. Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro


Yukiteru Ouji and Masahide Yoshikawa



19. Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro


Yukiteru Ouji and Masahide Yoshikawa




20. Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method


Ching-Hui Lin, Hao-Chen Chang, and Chia-Hsien Hsu



21. Automated Cell-Based Quantitation of 8-OHdG Damage


Bilge Debelec-Butuner, Aykut Bostancı, Lisa Heiserich, Caroline Eberle, Filiz Ozcan, Mutay Aslan, Dirk Roggenbuck, and Kemal Sami Korkmaz



22. CoCl2 Administration to Vascular MSC Cultures as an In Vitro Hypoxic System to Study Stem Cell Survival and Angiogenesis



Carmen Ciavarella, Silvia Fittipaldi, and Gianandrea Pasquinelli



23. Reporter Systems to Study Cancer Stem Cells


Caner Saygin, Mohamed Samour, Anastasia Chumakova, Awad Jarrar, Justin D. Lathia, and Ofer Reizes



24. Agent-Based Modeling of Cancer Stem Cell Driven Solid Tumor Growth


Jan Poleszczuk, Paul Macklin, and Heiko Enderling



25. Induction of a Tumor-Metastasis-Receptive Microenvironment as an Unwanted Side Effect After Radio/Chemotherapy and In Vitro and In Vivo Assays to Study This Phenomenon


Gabriela Schneider, Zachariah Payne Sellers, and Mariusz Z. Ratajczak



26. Isolation and Propagation of Glioma Stem Cells from Acutely Resected Tumors


Jin-Kyu Jung, Mark R. Gilbert, and Deric M. Park



27. Isolation and Characterization of Cancer Stem Cells of the Non-Small Cell Lung Cancer (A549) Cell Line



Nazilah Abdul Satar, Norashikin Zakaria, Noor Hanis Abu Halim, and Badrul Hisham Yahaya

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