T-Cell Differentiation: Methods and Protocols

T-Cell Differentiation: Methods and Protocols

by Enrico Lugli (Editor)

Paperback(Softcover reprint of the original 1st ed. 2017)

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Product Details

ISBN-13: 9781493982332
Publisher: Springer New York
Publication date: 06/27/2018
Series: Methods in Molecular Biology , #1514
Edition description: Softcover reprint of the original 1st ed. 2017
Pages: 271
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

The Vast Universe of T Cell Diversity: Subsets of Memory Cells and their Differentiation.- Basic Aspects of T Helper Cell Differentiation.- FACS Analysis of Memory T lymphocytes.- Intravital Microscopy Analysis of Hepatic T cell dynamics.- Analysis of T Cell Activation by Confocal Microscopy.- Phenotypic and Functional Analysis of Antigen-specific T Cell Exhaustion.- pMHC Multiplexing Strategy to Detect High Numbers of T Cell Responses in Parallel.- Differentiation of Diverse Progenies of Memory T cells from Naïve CD8+ T Cell Precursors.- Gammaretroviral Production and T Cell Transduction To Genetically Retarget Primary T Cells Against Cancer.- Measuring Telomerase Activity in Senescent Human T Cells upon Genetic Modification.- Strategies for T Helper Cell Subset Differentiation from Naïve Precursors.- Phenotypic and Functional Analysis of the Suppressive Function of Human Regulatory T Cells.- Approaches to Detect microRNA Expression in T Cell Subsets and T Cell Differentiation.- Next-generation Sequencing Analysis of Long Non-Coding RNAs in CD4+ T Cell Differentiation.- Highly Multiplexed, Single Cell Transcriptomic Analysis of T-cells by Microfluidic PCR.- Single-cell RNA Sequencing of Human T Cells.- Extensive Phenotypic Analysis, Transcription Factor Profiling, and Effector Cytokine Production of Human MAIT Cells by Flow Cytometry.- Developmental and Functional Assays to Study Murine and Human gd T Cells.

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