The Inflammasome: Methods and Protocols

The Inflammasome: Methods and Protocols

Paperback(Softcover reprint of the original 1st ed. 2013)

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Product Details

ISBN-13: 9781493960118
Publisher: Springer-Verlag New York, LLC
Publication date: 08/23/2016
Series: Methods in Molecular Biology , #1040
Edition description: Softcover reprint of the original 1st ed. 2013
Pages: 225
Product dimensions: 7.01(w) x 10.00(h) x 0.02(d)

Table of Contents

1. Assessing b-amyloid-induced NLRP3 Inflammasome Activation in Primary Microglia

Mareike Schnaars, Hannes Beckert, and Annett Halle

2. Activating the NLRP3 Inflammasome using the Amyloidogenic Peptide IAPP

Clara Westwell-Roper, Aisling Dunne, Man-Lyang Kim, C. Bruce Verchere, and Seth L. Masters

3. Assessment and Quantification of Crystal-induced Lysosomal Damage

Peter Duewell and Eicke Latz

4. Assessment of Inflammasome Activation in Primary Human Immune Cells

Theo S. Plantinga, Leo A.B. Joosten, and Mihai G. Netea

5. NLRP3 Inflammasome Activation and Cytotoxicity Induced by particulate Adjuvants

Marie Yang, Claire H.A. Hearnden, Ewa Oleszycka and Ed C. Lavelle

6. Measuring Inflammasome Activation in Response to Bacterial Infection

Petr Broz and Denise M. Monack

7. Detection of Pyroptosis by Measuring Released Lactate Dehydrogenase Activity

Manira Rayamajhi, Yue Zhang, and Edward A. Miao

8. ASC Speck Formation as a Read-out for Inflammasome Activation

Andrea Stutz, Gabor L. Horvath, Brian G. Monks, and Eicke Latz

9. Immunoblotting for Active Caspase-1

Christopher Jakobs, Eva Bartok, Andrej Kubarenko, Franz Bauernfeind and Veit Hornung

10. Inflammasome Activation and Inhibition in Primary Murine Bone Marrow-Derived Cells, and Assays for IL-1α, IL-1β and Caspase-1

Katharina S. Schneider1, Christina J. Thomas, and Olaf Groß

11. Reconstituting the NLRP1 Inflammasome in vitro

Benjamin Faustin and John C. Reed

12. Assessing ATP Binding and Hydrolysis by NLR Proteins

Jinyao Mo and Joseph A. Duncan

13. Blue Native Polyacrylamide Gel Electrophoresis to Monitor Inflammasome Assembly and Composition

Eric M. Kofoed and Russell E. Vance

14. Analysis of Microbiota Alterations in Inflammasome-deficient Mice

Eran Elinav, Christoph A Thaiss, and Richard A Flavell

15. Quantification of Adipose Tissue Leukocytosis in Obesity

Ryan Grant, Yun-Hee Youm, Anthony Ravussin, and Vishwa Deep Dixit

16. In vivo Evaluation of Neutrophil Recruitment in Response to Sterile Particulates

Tamiko Yanagida, Kaoru Orihashi and Hajime Kono

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