The Molecular Biology of Chloroplasts and Mihondria in Chlamydomonas provides a comprehensive coverage of chloroplast and mihondrial biogenesis and function. The book presents the current status of research on the molecular genetics of chloroplast gene expression. It describes the intricate cooperative interplay between the chloroplast and nuclear compartments which underlies the assembly of the photosynthetic apparatus. It also shows the power of chloroplast reverse genetics which has been used with considerable success to investigate structure-function relationships in several photosynthetic complexes. The book highlights the features of Chlamydomonas and the technological advances which make the alga uniquely suited to a multidisciplinary approach to study photosynthesis and its regulation under various environmental and stress conditions. The book is intended for a wide audience, but is specifically designed for advanced undergraduate and graduate students and researchers in the fields of biochemistry, molecular biology, physiology, biophysics, plant biology, phycology and biotechnology.
Table of ContentsPreface. Color Plates. 1. Introduction to Chlamydomonas; E.H. Harris. 2. Perspectives on Early Research on Photosynthesis in Chlamydomonas; R.K. Togasaki, S.J. Surzycki. 3. Organization of the Nuclear Genome; C.D. Silflow. 4. Nuclear Transformation: Technology and Applications; K.L. Kindle. 5. Modes and Tempos of Mitochondrial and Chloroplast Genome Evolution in Chlamydomonas: A Comparative Analysis; A.M. Nedelcu, R.W. Lee. 6. Uniparental Inheritance of Chloroplast Genomes; E.V. Armbrust. 7. Replication, Recombination, and Repair in the Chloroplast Genetic System of Chlamydomonas; B.B. Sears. 8. Chloroplast Transformation and Reverse Genetics; M. Goldschmidt-Clermont. 9. Chloroplast RNA Stability; J. Nickelsen. 10. Chloroplast RNA Synthesis and Processing; D.B. Stern, R.G. Drager. 11. RNA Splicing in the Chloroplast; D.L. Herrin, et al. 12. Regulation of Chloroplast Translation; C.R. Hauser, et al. 13. Chloroplast Protein Translocation; M.C. Perret, et al. 14. Supramolecular Organization of the Chloroplast and of the Thylakoid Membranes; J. Olive, F.-A. Wollman. 15. Assembly of Photosystem II; J.M. Erickson. 16. Functional Analysis of Photosystem II; S.V. Ruffle, R.T. Sayre. 17. Structure and Function of Photosystem I; A.N. Webber, S.E. Bingham. 18. Reexamining the Validity of the Z-Scheme: Is Photosystem I Required for Oxygenic Photosynthesis in Chlamydomonas? K. Redding, G. Peltier. 19. Assembly of Light-Harvesting Systems; J.K. Hoober, et al. 20. Pigment Biosynthesis: Chlorophylls, Heme, and Carotenoids; M.P. Timko. 21. Glycerolipids: Composition, Biosynthesis and Function in Chlamydomonas; A. Trémolières. 22. In vivo Measurements of Photosynthetic Activity: Methods; P. Joliot, et al. 23. New Digital Imaging Instrument for Measuring Fluorescence and Delayed Luminescence; P. Bennoun, D. Béal. 24. The Structure, Function and Biogenesis of Cytochrome b6f Complexes; F.-A. Wollman. 25. Assembly and Function of the Chloroplast ATP Synthase; H. Strotmann, et al. 26. Molecular Aspects of Components of the Ferredoxin/Thioredoxin Systems; J.-P. Jacquot, et al. 27. Genetic Engineering of Rubisco; R.J. Spreitzer. 28. CO2 Acquisition. Acclimation to Changing Carbon Availability; M.H. Spalding. 29. Regulation of Starch Biosynthesis; S.G. Ball. 30. State Transition and Photoinhibition; N. Keren, I. Ohad. 31. Synthesis of Metalloproteins Involved in Photosynthesis: Plastocyanin and Cytochromes; S. Merchant. 32. Responses to Deficiencies in Macronutrients; J.P. Davies, A.R. Grossman. 33. Nitrogen Assimilation and its Regulation; E. Fernández, et al. 34. Mitochondrial Genetics; C. Remacle, R.F. Matagne. 35. Chlororespiration, Sixteen Years Later; P. Bennoun. 36. Perspectives; L.J. Mets, J-D. Rochaix. Index.