The Renin-Angiotensin-Aldosterone System: Methods and Protocols

The Renin-Angiotensin-Aldosterone System: Methods and Protocols

by Sean E. Thatcher (Editor)

Hardcover(1st ed. 2017)

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Product Details

ISBN-13: 9781493970285
Publisher: Springer New York
Publication date: 05/13/2017
Series: Methods in Molecular Biology , #1614
Edition description: 1st ed. 2017
Pages: 201
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

Table of Contents…
Contributing Authors…

1. A Brief Introduction into the Renin-Angiotensin-Aldosterone System: New and Old Techniques
Sean E. Thatcher

2. A Color Segmentation Based Method to Quantify Atherosclerotic Lesion Compositions with Immunostaining
Congqing Wu, Alan Daugherty, and Hong Lu

3. Assessment of Protein Carbonylation and Protein Tyrosine Phosphatase (PTP) Oxidation in Vascular Smooth Muscle Cells (VSMCs) using Immunoblotting Approaches
Sofia Tsiropoulou and Rhian M. Touyz

4. Methods for Studying the Role of RAAS in the Modulation of Vascular Repair-Relevant Functions of Stem/Progenitor Cells
Yagna P.R. Jarajapu

5. Use of a Fluorescent Substrate to Measure ACE2 Activity in the Mouse Abdominal Aorta
Yu Wang, Lisa A. Cassis, and Sean E. Thatcher

6. Measuring Blood Pressure using a Non-Invasive Tail Cuff Method in Mice
Yu Wang, Sean E. Thatcher, and Lisa A. Cassis

7. Blood Pressure Monitoring using Radio Telemetry Method in Mice
Yu Wang, Sean E. Thatcher, and Lisa A. Cassis

8. Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry
Nathan P. Rudemiller and Steven D. Crowley

9. Assessment of the Renin-Angiotensin System in Cellular Organelle: New Arenas for Study in the Mitochondria
Bryan A. Wilson and Mark C. Chappell

10. Comprehensive Assessments of Energy Balance in Mice
Justin L. Grobe

11. In Vitro Assays to Determine Smooth Muscle Cell Hypertrophy, Protein Content, and Fibrosis
Katherine J. Elliott and Satoru Eguchi

12. A New Mouse Model for Introduction of Aortic Aneurysm by Implantation of Deoxycorticosterone Acetate Pellets or Aldosterone Infusion in the Presence of High Salt
Shu Liu, Ming C. Gong, and Zhenheng Guo

13. Fluorescence-Based Binding Assay for Screening Ligands of Angiotensin Receptors
Maiia E. Bragina, Nikolaos Stergiopulos, and Rodrigo A. Fraga-Silva

14. A Primer to Angiotensin Peptide Isolation, Stability, and Analysis by Nano-Liquid Chromatography with Mass Detection
Mariola Olkowicz, Stefan Chlopicki, and Ryszard T. Smolenski

15. Analysis of Angiotensin Metabolism in the Kidney using Mass Spectrometry
Nadja Grobe and Khalid M. Elased

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