Ubiquitin and Ubiquitin-like Protein Modifiers

by Mark Hochstrasser (Editor)

Overview

Ubiquitination and Protein Stability - Part A Volume 618, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapter written by an international board of authors. Topics of note in this new release include the Preparation of ubiquitinated nucleosomes with native and non-hydrolyzable linkages, Methods to measure ubiquitin chain length and linkage, Genetic approaches to study the yeast ubiquitin system, Enzymatic preparation of monoubiquitinated proteins, Methods to distinguish the function of ubiquitin in autophagy and the proteasome pathway, the Purification and characterization of enzyme activity of USPs, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in this series on enzymology - Updated release includes the latest information on methods to measure ubiquitin chain length and linkage, genetic approaches to study the yeast ubiquitin system, amongst many other timely topics

Product Details

ISBN-13:	9780128163603
Publisher:	Elsevier Science & Technology Books
Publication date:	03/06/2019
Series:	Methods in Enzymology , #618
Sold by:	Barnes & Noble
Format:	eBook
Pages:	430
File size:	30 MB
Note:	This product may take a few minutes to download.

About the Author

Dr. Mark W Hochstrasser is a Eugene Higgins Professor of Molecular Biophysics and Biochemistry and Professor of Molecular, Cellular, and Developmental BiologyYale University, USA.

Table of Contents

1. Semisynthesis of ubiquitinated histone H2B with a native or nonhydrolyzable linkageMichael Morgan, Muhammad Jbara, Ashraf Brik and Cynthia Wolberger2. Dual-color pulse-chase ubiquitination assays to simultaneously monitor substrate priming and extensionDaniel C. Scott and Brenda A. Schulman3. A genetic approach to study polyubiquitination in Saccharomyces cerevisiaeFernando Meza-Gutierrez, Deniz Simsek and David P. Toczyski4. Enzymatic preparation of monoubiquitinated FANCD2 and FANCI proteinsViduth K. Chaugule, Connor Arkinson, Rachel Toth and Helen Walden5. Methods to measure ubiquitin chain length and linkageFumiaki Ohtake, Hikaru Tsuchiya, Keiji Tanaka and Yasushi Saeki6. Detection of ubiquitination activity and identification of ubiquitinated substrates using TR-TUBEYukiko Yoshida, Yasushi Saeki, Hikaru Tsuchiya and Keiji Tanaka7. Methods to study phosphoribosylated ubiquitin ligation and removalJiazhang Qiu and Zhao-Qing Luo8. Biochemical characterization of SUMO-conjugating enzymes by in vitro sumoylation assaysNathalie Eisenhardt, Dragana Ilic, Easa Nagamalleswari and Andrea Pichler9. Methods to study SUMO dynamics in yeastStefan Pabst, Lennard-Maximilian Döring, Natasha Petreska and R. Jürgen Dohmen10. Approaches for investigating the extracellular signaling function of ISG15Caleb D. Swaim, Larissa A. Canadeo and Jon M. Huibregtse11. Analysis of modification and proteolytic targeting by the ubiquitin-like modifier FAT10Annette Aichem, Annika N. Boehm, Nicola Catone, Gunter Schmidtke and Marcus Groettrup12. Methods to analyze STUbL activityEmma Branigan, Anna Plechanovová and Ronald T. Hay13. Quantitative analysis of USP activity in vitroShreya Dharadhar, Robbert Q. Kim, Michael Uckelmann and Titia K. Sixma14. Evaluating enzyme activities and structures of DUBsJonathan N. Pruneda and David Komander15. Purification and functional characterization of the DUB domain of SdeAKedar Puvar, Shalini Iyer, Michael J. Sheedlo and Chittaranjan Das16. Profiling DUBs and Ubl-specific proteases with activity-based probesPaul P. Geurink, Gerbrand J. van der Heden van Noort, Monique P.C. Mulder, Robert C.M. Knaap, Marjolein Kikkert and Huib Ovaa17. Assays of SUMO protease/isopeptidase activity and function in mammalian cells and tissuesKathrin Kunz, Stefan Müller and Luca Mendler

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