Virus-Host Interactions: Methods and Protocols

Virus-Host Interactions: Methods and Protocols

Paperback(Softcover reprint of the original 1st ed. 2013)

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Product Details

ISBN-13: 9781493963089
Publisher: Springer-Verlag New York, LLC
Publication date: 08/23/2016
Series: Methods in Molecular Biology , #1064
Edition description: Softcover reprint of the original 1st ed. 2013
Pages: 372
Product dimensions: 7.01(w) x 10.00(h) x 0.03(d)

Table of Contents

1. A High-Throughput Yeast Two Hybrid Protocol to Determine Virus-Host Protein Interactions

Hannah Striebinger, Manfred Kögl, and Susanne M. Bailer

2. Analysis of Protein-Protein Interactions Using LUMIER Assay

Sonja Blasche and Manfred Koegl

3. Detection of Protein Interactions During Virus Infection by Bimolecular Fluorescence Complementation

Stefan Becker and Jens von Einem

4. Discovery of Host-Viral Protein Complexes During Infection

Daniell L. Rowles, Scott S. Terhune, and Ileana M. Cristea

5. Screening for Host Proteins with Pro- and Antiviral Activity Using High-Throughput RNAi

Samantha J. Griffiths

6. High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay by Metabolic Labelling of Newly Transcribed RNA using 4-thiouridine

Lars Dölken

7. Isolation and Characterization of Pathogen-Bearing Endosomes Enables Identification of New Cellular Cofactors and Analysis of Endosomal Escape

Konstanze D. Scheffer, Ruth Popa-Wagner and Luise Florin

8. Computational Analysis of Virus-Host Interactomes

Caroline C. Friedel

9. Interspecies Heterokaryon Assay to Characterize the Nucleocytoplasmic Shuttling of Herpesviral Proteins

Shuai Wang, Kezheng Wang, andChunfu Zheng

10. Detection of Integrated Herpesvirus Genomes by Fluorescent in situ Hybridization (FISH)

Benedikt Kaufer

11. Fast Generation of Stable Cell Lines Expressing Fluorescent Marker Molecules to Study Pathogen Induced Processes

Jens Bernhard Bosse, Jessica Ragues, and Harald Wodrich

12. Determination of HSV-1 Infectivity Using a Luciferase Reporter Cell Line

Diana Lieber and Susanne M. Bailer

13. Generation of a Cell Line for Constitutive Expression of Host or Viral miRNAs

Diana Lieber

14. Applications for a Dual Fluorescent Human Cytomegalovirus in the Analysis of Viral Entry

Kerstin Laib Sampaio, Gerhard Jahn, and Christian Sinzger

15. High Temporal Resolution Imaging Reveals Endosomal Membrane Penetration and Escape of Adenoviruses in Real-Time

Ruben Martinez, Andrew M. Burrage, Christopher M. Wiethoff, and Harald Wodrich

16. Three-Dimensional Visualization of Virus Infected Cells by Serial Sectioning - An Electron Microscopic Study Using Resin Embedded Cells

Martin Schauflinger,Clarissa Villinger, andPaul Walther

17. 3D Tissue Model for Herpes Simplex Virus-1 Infections

Ina Hogk, Steffen Rupp, and Anke Burger-Kentischer

18. In vivo Visualization of Encephalitic Lesions in Herpes Simplex Virus Type 1 (HSV-1) Infected Mice by Magnetic Resonance Imaging (MRI)

Wali Hafezi and Verena Hoerr

19. Detection of Antigen-Specific T Cells Based on Intracellular Cytokine Staining Using Flow-Cytometry

Tina Schmidt and Martina Sester

20. Functional NK Cell Cytotoxicity Assays Against Virus Infected Cells

Rebecca J. Aicheler and Richard J. Stanton

21. Assessment of Natural Killer Cell Responses to Human Cytomegalovirus Infected Macrophages

Zeguang Wu, Giada Frascaroli, and Thomas Mertens

22. Analysis of Cell Migration During HCMV Infection

Stefania Varani and Giada Frascaroli

23. Gene Targeting in Mice: A Review

Hicham Bouabe and Klaus Okkenhaug

24. A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant ES Cells

Hicham Bouabe and Klaus Okkenhaug

25. Measurement of Mouse Cytomegalovirus Induced Interferon-β with Immortalized Luciferase Reporter Cells

Evgenia Scheibe, Stefan Lienenklaus, Tobias May, Vladimir Goncalves Magalhaes, Siegfried Weiss, and Melanie M. Brinkmann

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