Apoptosis and Cancer: Methods and Protocols / Edition 1 by Gil Mor | 9781588294579 | Hardcover | Barnes & Noble
Apoptosis and Cancer: Methods and Protocols / Edition 1

Apoptosis and Cancer: Methods and Protocols / Edition 1

by Gil Mor
     
 

ISBN-10: 1588294579

ISBN-13: 9781588294579

Pub. Date: 11/09/2007

Publisher: Springer-Verlag New York, LLC

The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizing the mechanisms of apoptosis, as well as the industry laboratory, aimed at identifying new target molecules or

Overview

The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizing the mechanisms of apoptosis, as well as the industry laboratory, aimed at identifying new target molecules or screening for new compounds with potential clinical use.

Product Details

ISBN-13:
9781588294579
Publisher:
Springer-Verlag New York, LLC
Publication date:
11/09/2007
Series:
Methods in Molecular Biology Series, #414
Edition description:
2008
Pages:
266
Product dimensions:
5.98(w) x 9.02(h) x 0.04(d)

Table of Contents

APOPTOSIS AND CANCER Methods and Protocols Methods in Molecular Medicine Editors: Gil Mor MD, PhD Department of Obstetrics, Gynecology and Reproductive Science Yale University School of Medicine and Ayesha Alvero MD Department of Obstetrics, Gynecology and Reproductive Science Yale University School of Medicine Table of Contents: Chapter 1 (review) Modulation of Apoptosis to Reverse Chemoresistance Gil Mor, Michele K. Montagna and Ayesha B. Alvero Chapter 2. Caspase-3 Activation is a Critical Determinant of Genotoxic Stress-Induced Apoptosis Suparna Mazumder1, Dragos Plesca1,3, and Alexandru Almasan1,2, Chapter 3. Flow cytometry enumeration of apoptotic cancer cells by apoptotic rate *David Diaz, BSc *Alfredo Prieto BSc, PhD, *, Eduardo Reyes, BSc, PhD, *Hugo Barcenilla, BSc, *Jorge Monserrat, BSc, PhD, *, Melchor Alvarez-Mon, MD, PhD. Chapter 4 Detection of cancer-related proteins in fresh-frozen ovarian cancer samples using Laser capture microdissection Dan-Arin Silasi, Ayesha B. Alvero, Jechiel Mor, Rui Chen, Han-Hsuan Fu, Michele K. Montagna and Gil Mor Chapter 5 Flow cytometric detection of activated caspase-3 Richard Fox1 and Martine Aubert2 Chapter 6 The ChemoFx® Assay: An Ex Vivo Chemo-Sensitivity and Resistance Assay for Predicting Patient Response to Cancer Chemotherapy Stacey L. Brower, Ph.D., Jeffrey E. Fensterer, Jason E. Bush, Ph.D., MBA Chapter 7 Correlation of caspase activity and in vitro chemo-response in epithelial ovarian cancer cell lines Ayesha B. Alvero, Michele K. Montagna, and Gil Mor Chapter 8 Assessing Expression of Apoptotic Markers Using Large Cohort Tissue Microarrays Elah Pick, Mary M. McCarthy and Harriet M. Kluger Chapter 9 Mitochondria potential, Bax 'activation' and programmed cell death. C. Michael Knudson and Nicholas M. Brown Chapter 10 In Vitro and In Vivo Apoptosis Detection Using Membrane Permeant, Fluorescent Labeled Inhibitors of Caspases Brian W. Lee*, Michael R. Olin, Gary L. Johnson, and Robert J. Griffin Chapter 11 Caspase Activity Assays Andrew L. Niles, Richard A. Moravec, Terry L. Riss Chapter 12 Multiplex Caspase Activity and Cytotoxicity Assays Andrew L. Niles, Richard A. Moravec, Terry L. Riss Chapter 13 Homogeneous, Bioluminescent Proteasome Assays Martha A. O'Brien, Richard A. Moravec, Terry Riss, and Robert F. Bulleit Chapter 14 A simple method for profiling miRNA expression Jia-Wang Wang, PhD*, Jin Q. Cheng, Ph.D., M.D*. Chapter 15 Apoptotic Caspase Activation and Activity Jean-Berard Denault and Guy S. Salvesen Chapter 16 Biochemical Analysis of the native TRAIL Death-inducing Signalling Complex (DISC) Henning Walczak and Tobias L. Haas Chapter 17 Laser Microdissection Sample Preparation for RNA Analyses Christopher J. Vega

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