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Cartilage and Osteoarthritis / Edition 1

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ISBN-10:: 1617374504
ISBN-13:: 9781617374500
Pub. Date:: 11/10/2010
Publisher:: Springer-Verlag New York, LLC

ISBN-10:: 1617374504
ISBN-13:: 9781617374500
Pub. Date:: 11/10/2010
Publisher:: Springer-Verlag New York, LLC

Cartilage and Osteoarthritis / Edition 1

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Overview

Osteoarthritis (OA), the most common form of arthritis, is generally characterized by a slowly progressive degeneration of articular cartilage, particularly in the weight-bearing joints. It has a stronger prevalence in women, and its incidence increases with age. OA is a major and growing health concern in developed countries, owing to steadily increasing life expectancy and the demand for better quality of life. Because of its chronic nature and nonfatal outcome, OA affects the growing population of the elderly over an increasing time span. Moreover, despite its relatively benign character, OA is one of the most disabling diseases; it is responsible for increasing financial and social burdens in terms of medical treatments, forced inactivity, loss of mobility, and dependence. Despite a growing awareness of OA as a medical problem that has yet to reach its maximum impact on society, there is a surprising absence of effective medical treatments beyond pain control and surgery. So far, only symptom-modifying drugs are available, while there remains a major demand for disease-modifying treatments of proven clinical efficacy. This demand will hopefully be met in the future by some of the drugs that have been pressed into development and are now at different stages of clinical investigation. Nevertheless, the current lack of effective treatments reflects a still insufficient knowledge of cartilage with respect to its metabolism, interactions with other joint tissues, and causes and mechanisms (possibly of very different nature) leading to failure of its turnover.

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Product Details

ISBN-13:	9781617374500
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/10/2010
Series:	Methods in Molecular Medicine , #100
Edition description:	Softcover reprint of hardcover 1st ed. 2004
Pages:	358
Product dimensions:	5.98(w) x 9.02(h) x 0.03(d)

Culture and Phenotyping of Chondrocytes in Primary Culture Sylvie Thirion and Francis Berenbaum Culture of Chondrocytes in Alginate Beads Frédéric De Ceuninck, Christophe Lesur, Philippe Pastoureau, Audrey Caliez, and Massimo Sabatini Immortalization of Human Articular Chondrocytes for Generation of Stable, Differentiated Cell Lines Mary B. Goldring Culture of Immortalized Chondrocytes and Their Use As Models of Chondrocyte Function Mary B. Goldring Generation of Pluripotent Stem Cells and Their Differentiation to the Chondrocytic Phenotype Luis A. Solchaga, Jean F. Welter, Donald P. Lennon, and Arnold I. Caplan Semiquantitative Analysis of Gene Expression in Cultured Chondrocytes by RT-PCR Gaëlle Rolland-Valognes Quantification of mRNA Expression Levels in Articular Chondrocytes With PCR Technologies Audrey McAlinden, Jochen Haag, Brigitte Bau, Pia M. Gebhard, and Thomas Aigner RNA Extraction From Cartilage Frédéric Mallein-Gerin and Jérôme Gouttenoire Gene Expression Analysis in Cartilage by In Situ Hybridization Frédéric Mallein-Gerin and Jérôme Gouttenoire Analysis of Differential Gene Expression in Healthy and Osteoarthritic Cartilage and Isolated Chondrocytes by Microarray Analysis Thomas Aigner, Joachim Saas, Alexander Zien, Ralf Zimmer, Pia M. Gebhard, and Thomas Knorr High-Efficiency Nonviral Transfection of Primary Chondrocytes Jean F. Welter, Luis A. Solchaga, and Matthew C. Stewart In Vitro Gene Transfer to Chondrocytes and Synovial Fibroblasts by Adenoviral Vectors Jean-Noel Gouze, Martin J. Stoddart, Elvire Gouze, Glyn D. Palmer, Steven C. Ghivizzani, Alan J. Grodzinsky, and Christopher H. Evans Changes of Chondrocyte Metabolism In Vitro: An Approach by Proteomic Analysis Anne-Marie Freyria and Michel Becchi Analysis of ChondrocyteFunctional Markers and Pericellular Matrix Components by Flow Cytometry Gust Verbruggen, Jun Wang, Lai Wang, Dirk Elewaut, and Eric M. Veys A Simple and Reliable Assay of Proteoglycan Synthesis by Cultured Chondrocytes Frédéric De Ceuninck and Audrey Caliez Assays of Proteoglycan and Collagen Degradation in Cultures of Rabbit Cartilage Explants Christophe Lesur and Massimo Sabatini Production of Antibodies Against Degradative Neoepitopes in Aggrecan John S. Mort and Peter J. Roughley Immunoassays for Collagens in Chondrocyte and Cartilage Explant Cultures R. Clark Billinghurst, Fackson Mwale, Anthony Hollander, Mirela Ionescu, and A. Robin Poole Detection of Apoptosis in Cartilage In Situ and in Isolated Chondrocytes Darryl D. D'Lima, Klaus Kuhn, and Martin K. Lotz Expression, Activity, and Regulation of MAP Kinases in Cultured Chondrocytes Jang-Soo Chun Mechanical Loading of Chondrocytes Embedded in 3D Constructs: In Vitro Methods for Assessment of Morphological and Metabolic Response to Compressive Strain David A. Lee and Martin M. Knight In Vitro Physical Stimulation of Tissue-Engineered and Native Cartilage Kelvin W. Li, Travis J. Klein, Kanika Chawla, Gayle E. Nugent, Won C. Bae, and Robert L. Sah Index

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Editorial Reviews

3 Stars from Doody

Reviewer: James C Byrd, MD, PhD (Washington University Medical Center)
Description: This book is a compilation of laboratory methods used in the study of cartilage and its role in the pathogenesis of osteoarthritis. Up-to-date methods from cell culture to molecular biology approaches are emphasized, with the different methods subdivided into individual chapters.
Purpose: This is volume one (of two) in a series devoted to methods of studying the role of cartilage in osteoarthritis. It is primarily directed at an audience familiar with a research laboratory and focuses on molecular and cellular biology approaches, while the second volume promises to focus on tissue level experiments of diseased articular cartilage. Essentially, the authors have developed a manual providing step-by-step techniques for the study of chondrocytes including how-to instructions from chondrocyte isolation to genetic manipulation. While this information can be found in the scientific literature, this provides a concise review of emerging techniques that are essential in the study of osteoarthritis, meeting the objective goals of the authors.
Audience: It is directed at anyone who works in a research laboratory conducting experiments directed at rheumatologic disease. The contributors are scientists from universities and research laboratories from around the world and represent a large body of collective knowledge in the techniques used to dissect out the molecular mechanisms of chondrocyte biology.
Features: The authors use a repeating chapter structure that includes a brief introduction to each technique followed by step-by-step instructions in outline form to provide the reader with the information necessary to repeat these methods in a laboratory setting. The techniques described include methods necessary for the culture of chondrocyte cell lines, molecular techniques for the study of gene expression and touches on the biochemical analysis of important target molecules. The book does a particularly good job of describing the techniques of chondrocyte isolation and culture, but only addresses selected topics about their study. Several clear illustrations are including in almost every chapter to help the reader visualize these complex experiment paradigms.
Assessment: This is a high quality laboratory manual that provides detailed instructions for conducting experiments directed at studying the role of cartilage in osteoarthritis. Overall, this would be a worthwhile book to keep in the laboratory for anyone studying the molecular mechanisms of connective tissue diseases.

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