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Nonviral Vectors for Gene Therapy: Methods and Protocols / Edition 1

Nonviral Vectors for Gene Therapy: Methods and Protocols / Edition 1

by Mark A. Findeis
Nonviral Vectors for Gene Therapy: Methods and Protocols / Edition 1
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ISBN-10:
0896037126
ISBN-13:
9780896037120
Pub. Date:
08/09/2001
Publisher:
Springer-Verlag New York, LLC
ISBN-10:
0896037126
ISBN-13:
9780896037120
Pub. Date:
08/09/2001
Publisher:
Springer-Verlag New York, LLC
Nonviral Vectors for Gene Therapy: Methods and Protocols / Edition 1

Nonviral Vectors for Gene Therapy: Methods and Protocols / Edition 1

by Mark A. Findeis

Overview

The purpose of this volume of Methods in Molecular Medicine is to set forth examples of the great variety of techniques and applications that are now emerging in the field of nonviral gene therapy. The book emphasizes not only specific approaches to gene delivery but, in particular, the best current me- ods to prepare, handle, and characterize gene delivery agents. These topics are of very broad importance since gene therapy evolves from its mostly ac- emy-based experimental and clinical research to the ever increasing number of industry-driven programs directed toward commercial development. S- cessful introduction of nonviral gene therapy agents into the clinic should be expected to require rigorous manufacturing and analytical methods that readily meet the regulatory guidelines under which new drug candidates are reviewed for marketing approval. Exactly what those guidelines will prove to be c- tainly depends on the established guidelines for review of both biological and chemical therapeutics. Additionally, many new techniques are being devised and applied to gene therapy research; these techniques will be instrumental in developing and characterizing successful gene delivery agents. Nonviral Vectors for Gene Therapy: Methods and Prools has two main sections. To start with, there is a series of chapters on specific prools for the synthesis, characterization, and application of gene delivery agents. S- eral chapters address the topic of materials to bind with DNA to form the compact condensed phases that facilitate cellular delivery.

Product Details

ISBN-13: 9780896037120
Publisher: Springer-Verlag New York, LLC
Publication date: 08/09/2001
Series: Methods in Molecular Medicine , #65
Edition description: 2001
Pages: 399
Product dimensions: 6.10(w) x 9.25(h) x 0.04(d)

About the Author

Findeis, Mark A.

Table of Contents

Synthesis of Polyampholyte Comb-Type Copolymers Consisting of a Poly(L-lysine) Backbone and Hyaluronic Acid Side Chains for DNA Carrier.- Cationic—-Helical Peptides for Gene Delivery into Cells.- Supramolecular Self-Assembly of Poly(ethylene glycol)-Block-Poly (L-lysine) Dendrimer with Plasmid DNA.- Water-Soluble Cationic Methacrylate Polymers for Nonviral Gene Delivery.- Stabilization of Polycation-DNA Complexes by Surface Modification with Hydrophilic Polymers.- Use of Disulfide Cationic Lipids in Plasmid DNA Delivery.- Interactions of Lipid-Oligonucleotide Conjugates with Low-Density Lipoprotein.- Coupling of Nuclear Localization Signals to Plasmid DNA.- Progress Toward a Synthetic Virus.- Characterization of Polyampholyte Comb-Type Copolymer DNA Carriers.- Methods for Studying the Formation of Polycation-DNA Complexes and Properties Useful for Gene Delivery.- Characterization of DNA Condensates by Atomic Force Microscopy.- Rapid and Systematic Transfer and Recovery of Large BACs/PACs into Mammalian Cells by HAEC Retrofitting.- Systemic Delivery of Therapeutic Proteins by Intramuscular Injection of Plasmid DNA.- Local Delivery of Therapeutic Proteins by Intratumoral Injection of Plasmid DNA-Lipid Complexes.- Nonviral DNA Delivery from Polymeric Systems.- Promotion of Duplex and Triplex DNA Formation by Polycation Comb-Type Copolymers.- Lyophilization of Nonviral Gene Delivery Systems.- Ultraviolet Absorption and Circular Dichroism Spectroscopy of Nonviral Gene Delivery Complexes.- Characterization of Synthetic Gene Delivery Vectors by Infrared Spectroscopy.- Characterization of Cationic Vector-Based Gene Delivery Vehicles Using Isothermal Titration and Differential Scanning Calorimetry.- Light-Scattering Techniques for Characterization of Synthetic Gene TherapyVectors.- Renal Gene Therapy.
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