Adenovirus Methods and Protocols: Volume 2: Ad Proteins and RNA, Lifecycle and Host Interactions, and Phyologenetics

Adenovirus Methods and Protocols: Volume 2: Ad Proteins and RNA, Lifecycle and Host Interactions, and Phyologenetics

Hardcover(2nd ed. 2007)

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Adenovirus Methods and Prools, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. The prools presented are written by trendsetting researchers.

Product Details

ISBN-13: 9781588299017
Publisher: Springer-Verlag New York, LLC
Publication date: 02/26/2007
Series: Methods in Molecular Medicine , #131
Edition description: 2nd ed. 2007
Pages: 362
Product dimensions: 6.10(w) x 9.25(h) x 0.03(d)

Table of Contents

Ann E. Tollefson, Mohan Kuppuswamy, and William S. M. Wold TABLE OF CONTENTS Vol. II Topics: Ad proteins and RNA, lifecycle and host interactions, and phylogenetics

1. Analysis of the Efficiency of Adenovirus Transcription Cristina Iftode and S. J. Flint
2. The Use of In Vitro Transcription to Probe Regulatory Functions of Viral Protein Domains Paul M. Loewenstein, Chao-Zhong Song and Maurice Green
3. Preparation of Soluble Extracts from Adenovirus Infected Cells for Studies of RNA Splicing Oliver Mühlemann and Göran Akusjärvi
4. In Vitro Methods to Study RNA Interference During an Adenovirus Infection Gunnar Andersson, Ning Xu and Göran Akusjärvi
5. Simultaneous Detection of Adenovirus RNA and Cellular Proteins by Fluorescent Labeling In Situ Eileen Bridge
6. Study of Nucleolar Localization of Adenovirus Core Proteins David A. Matthews
7. Analysis of Adenovirus Infections in Synchronized Cells David A. Ornelles, Robin N. Shepard and Felicia D. Goodrum
8. Co-Immunoprecipitation of Protein Complexes Peter Yaciuk
9. Chromatin Immunoprecipitation (ChIP) to Study the Binding of Proteins to the Adenovirus Genome in Vivo Jihong Yang and Patrick Hearing
10. Assaying Protein-DNA Interactions In Vivo and In Vitro Using Chromatin Immunoprecipitation and Electrophoretic Mobility Shift Assays Pilar Perez-Romero and Michael J. Imperiale
11. Identifying Functional Adenovirus-Host Interactions Using Tandem Mass Spectrometry Anuj Gaggar, Dmitry Shayakhmetov, and Andre Lieber
12. The Use of Cell Microinjection for the in vivo Analysis of Viral Transcriptional Regulatory Protein Domains Maurice Green, Andrew Thorburn, Robert Kern and Paul M. Loewenstein
13. Determination of the Transforming Activities of Adenovirus Oncogenes Michael Nevels and Thomas Dobner
14. Human Adenovirus Type 12: Crossing Species Barriers to Immortalize the Viral Genome Walter Doerfler
15. Measurement of NK Cell Lytic Activity of Adenovirus-Infected or Adenovirus-Transformed Cells John M. Routes
16. A Flow Cytometric Assay for Analysis of NK Cell-Mediated Cytolysis of Adenovirus-Transformed Cells Graham Bottley, Graham P. Cook, and G. Eric Blair
17. Large Scale Purification and Crystallization of Adenovirus Hexon John J. Rux and Roger M. Burnett
18. Synthesis and Assay of Recombinant Adenovirus Protease Joseph M. Weber
19. Assay for the Adenovirus Proteinase- Purification of the Enzyme and Synthesis of a Fluorogenic Substrate Walter F. Mangel and William J. McGrath
20. Cofactors of the Adenovirus Proteinase: Measuring Equilibrium Dissociation Constants and Stoichiometries of Binding Walter F. Mangel and William J. McGrath
21. Characterization of the Adenovirus Fiber Protein Jeffrey A. Engler and Jeong Shin Hong
22. Phylogenetic Analysis of Adenovirus Sequences Balázs Harrach and Mária Benkö
23. Assessment of Genetic Variability Among Subspecies B1 Human Adenoviruses for Molecular Epidemiology Studies Adriana E. Kajon and Dean D. Erdman

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