Affinity Biosensors: Techniques and Protocols / Edition 1

by Kim Rogers, Ashok Mulchandani

Overview

The frequency of reports concemmg the interface of biological reco- tion elements to signal transduction technologies has risen dramatically over the last decade. Because any one of a wide variety of biological recognition elements (e. g. , antibodies, receptors, DNA, microorganisms, or enzymes) can theoretically be interfaced with any one of a wide variety of signal transducers (e. g. , optical, electrochemical, thermal, or acoustic), the potential range of devices and techniques can be bewildering. The purpose of this volume and the previous volume in this series is to provide a basic reference and startmg point for investigators in academics, mdustry, and government to begin or expand their biosensors research. This volume, Methods in Biotechnology vol. 7: Affinity Biosensors: Techniques and Prools, describes a variety of classical and emerging transduction technologies that have been interfaced to bioaffinity elements (e. g. , antibodies and receptors). Some of the reasons for the expansion in the use of affinity-based biosensors include both advances in signal transduction technologies (e. g. , fiber optics, microelectromcs, and microfabrication) and the availability of bioafflmty elements. More specifically, with respect to biological recognttion elements, commercially and noncommercially produced antibodies directed toward a variety of analytes have become widely available. In addition, te- niques for the purification and stabilization of receptors have also significantly improved. As a result of these recent advances in the field, biosensors research and development projects are being pursued by mvestigators from a wide range of disciplines.

Product Details

ISBN-13:	9781617370663
Publisher:	Springer-Verlag New York, LLC
Publication date:	10/08/2011
Series:	Methods in Biotechnology , #7
Edition description:	1998
Pages:	249
Product dimensions:	5.98(w) x 9.02(h) x 0.02(d)

About the Author

Rogers, Kim R. (U.S. Environmental Protection Agency); Mulchandani, Ashok (UC Riverside)

Table of Contents

Enzyme Biosensors.- Principles of Affinity-Based Biosensors.- Immunobiosensors Based on Thermistors.- Affinity Biosensing Based on Surface Plasmon Resonance Detection.- Immunosensors Based on Piezoelectric Crystal Device.- Immunobiosensors Based on Evanescent Wave Excitation.- A galactose-specific affinity hollow fiber sensor based on fluorescence resonance energy transfer.- Fiberoptic immunosensors with continuous analyte response.- Immunobiosensors based on grating couplers.- Receptor biosensors based on optical detection.- Biosensor-Related Techniques.- lmmunobiosensors based on ion-selective electrodes.- Biosensors based on DNA intercalation using light polarization.- ISFET affinity sensor.- Liposome-Based immunomigration assays.- Isolated receptor biosensors based on bilayer lipid membranes.- Eukaryotic cell biosensor The Cytosensor microphysiometer.

Editorial Reviews

"I enthusiastically recommend this book for anyone involved in the biosensor fields. . .allows one to quickly become knowledgeable in the field. . .very well written and organized, and it has a good index. The chapters are written by the noted experts who have developed each sensor type. . . an essential complement to its companion volume, which covers enzyme and microbial sensors."-John I. Peterson, NIH reviewing for Analytical Chemistry

From the Publisher

3 Stars from Doody

This is an overview of the principles relevant to the design and operational features of affinity-based biosensors and related techniques. This volume provides a basic reference and starting point for investigators in academics and industry to begin or expand their biosensors research. It is aimed at a large and varied audience, including graduate students and postdoctoral and senior researchers interested in the field of biosensors. A variety of classical and emerging transduction technologies that have been interfaced with bioaffinity elements are described. This volume, although not an exhaustive treatise, provides a detailed, step-by-step description for a variety of affinity-based biosensor technologies that will allow the novice or the experienced investigator to expand into new areas of research most appropriate for their analytical needs. In addition, detailed protocols, special notes, and safety items have been included to provide details that may not normally appear in journal articles. This book is very useful for detailed methodologies and theories related to biosensors for use in analytical techniques. To the researcher or the technician, this volume, along with Volume 6, is a necessity for a full understanding of biosensor techniques.

Howard H. Sky-Peck

Collects 15 articles on techniques and protocols for constructing, evaluating, and using affinity biosensors. Representative topics include immunosensors based on piezoelectric crystal device, receptor biosensors based on optical detection, biosensors based on DNA intercalation using light polarization, and isolated receptor biosensors based on bilayer lipid membranes. Designed for researchers wanting to use an affinity biosensor for a specific analytical measurement and for novices beginning research in their field. Annotation c. by Book News, Inc., Portland, Or.

Booknews

Reviewer: Howard H. Sky-Peck, MD, PhD, FRSM (Rush Medical College of Rush University)
Description: This is an overview of the principles relevant to the design and operational features of affinity-based biosensors and related techniques.
Purpose: This volume provides a basic reference and starting point for investigators in academics and industry to begin or expand their biosensors research.
Audience: It is aimed at a large and varied audience, including graduate students and postdoctoral and senior researchers interested in the field of biosensors.
Features: A variety of classical and emerging transduction technologies that have been interfaced with bioaffinity elements are described. This volume, although not an exhaustive treatise, provides a detailed, step-by-step description for a variety of affinity-based biosensor technologies that will allow the novice or the experienced investigator to expand into new areas of research most appropriate for their analytical needs. In addition, detailed protocols, special notes, and safety items have been included to provide details that may not normally appear in journal articles.
Assessment: This book is very useful for detailed methodologies and theories related to biosensors for use in analytical techniques. To the researcher or the technician, this volume, along with Volume 6, is a necessity for a full understanding of biosensor techniques.

Doody's Review Service