Antibiotics: Methods and Protocols

by Peter Sass

Overview

This volume provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, and methods for the analysis and detection of microbial adaptation strategies. Antibiotics: Methods and Prools guides readers through chapters on production and design, mode of action, and response and susceptibility. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Antibiotics: Methods and Prools aims to inspire scientific work in the exciting field of antibiotic research.

Product Details

ISBN-13:	9781493982554
Publisher:	Springer New York
Publication date:	06/27/2018
Series:	Methods in Molecular Biology , #1520
Edition description:	Softcover reprint of the original 1st ed. 2017
Pages:	358
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Antibiotics - Precious Goods in Changing Times.- Mining Bacterial Genomes for Secondary Metabolite Gene Clusters.- Production of Antimicrobial Compounds by Fermentation.- Structure Elucidation of Antibiotics by NMR Spectroscopy.- Computer-Aided Drug Design Methods.- Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents.- Application of a Bacillus subtilis Whole-cell Biosensor (_liaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds.- Determination of Bacterial Membrane Impairment by Antimicrobial Agents.- Mass-sensitive Biosensor Systems to Determine the Membrane Interaction of Analytes.- Measurement of Cell Membrane Fluidity by Laurdan GP:Fluorescence Spectroscopy and Microscopy.- In vitro Assays to Identify Antibiotics Targeting DNA Metabolism.- Fluorescence-based real-time Activity Assays to Identify RNase P inhibitors.- Reporter Gene-based Screening for TPP Riboswitch Activators.- Cell-based Fluorescent Screen to Identify Inhibitors of Bacterial Translation Initiation.-Bacterial Histidine Kinases: Overexpression, Purification, and Inhibitor Screen.- Expression Profiling of Antibiotic Resistant Bacteria Obtained by Laboratory Evolution.- Sample Preparation for Mass-Spectrometry Based Absolute Protein Quantification in Antibiotic Stress Research.- Label-Free Quantitation of Ribosomal Proteins from Bacillus Subtillis for Antibiotic Research.- Functional Metagenomics to Study Antibiotic Resistance.- Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains using High Throughput Sequencing.