Antibody Engineering Protocols

by Sudhir Paul (Editor)

Overview

This comprehensive collection of recently developed methods for producing new antibody reagents by immunization and recombinant DNA techniques contains ready-to-use prools that illuminate current areas of research on antibody structure, functions, and applications. The methods can be applied in basic immunological studies involving antibody specificity, catalysis, and evolution, and in the isolation of rare antibodies by phage display technology and the engineering of new antibodies by mutagenesis. They offer insight into new ways of developing clinically useful antibody reagents.

Antibody Engineering Prools constitutes a single-source volume for laboratory investigators who want to minimize extensive literature and methodology searches and to work productively in their fields with reproducible step-by-step prools.

Product Details

ISBN-13:	9780896032750
Publisher:	Springer-Verlag New York, LLC
Publication date:	07/13/1995
Series:	Methods in Molecular Biology , #51
Edition description:	1995
Pages:	450
Product dimensions:	6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

SEQHUNT.- Molecular Modeling of Antibody-Combining Sites.- Structure and Properties of Human Immunoglobulin Light-Chain Dimers.- Crystallographic and Chromatographic Methods for Study of Antibody Light Chains and Other Proteins.- Detection of Human Variable Gene Family Expression at the Single-Cell Level.- Purification of Reduced and Alkylated Antibody Subunits.- Murine Monoclonal Antibody Development.- Comparative Properties of Polyclonal and Monoclonal Antibodies.- Evaluation of Antibody Clonality.- Affinity Immunoblotting.- Epitope and Idiotope Mapping Using Monoclonal Antibodies.- Anti-Idiotypic Antibodies That Mimic Opioids.- Catalytic Antibodies.- Preparation and Assay of Acetylcholinesterase Antibody.- DNA Hydrolysis by Antibodies.- Screening Strategies for Catalytic Antitransition-State Analog Antibodies.- Expression of Chimeric Immunoglobulin Genes in Mammalian Cells.- Single-Chain Anti-DNA Fv.- Molecular Cloning of Antiground-State Proteolytic Antibody Fragments.- Cloning and Bacterial Expression of an Esterolytic sFv.- Site-Directed Mutagenesis of Antibody-Variable Regions.- Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation.- Chaperonins in Phage Display of Antibody Fragments.- Phage-Display Libraries of Murine and Human Antibody Fab Fragments.- Selection of Human Immunoglobulin Light Chains from a Phage-Display Library.- Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography.- Rapid Purification of Recombinant Antibody Fragments for Catalysis Screening.- Assay of Radiolabeled VIP Binding and Hydrolysis by Antibodies.- Methods of Measuring Thyroglobulin and Peptide-Methylcoumarinamide Hydrolysis by Autoantibodies.- Radiolabeling of Antibodies for Therapy and Diagnosis.

Editorial Reviews

This is a new book containing procedures for the production of various forms of antibodies plus their analysis, use, and characterization. The preface does not contain any particular goals for this book. The title implies that the book will provide protocols for the molecular engineering of antibodies. The unique subjects covered in this text are a welcome addition to the research field. The book does contain some protocols for working with antibodies, but the reader must be very knowledgeable of the molecular biology involved with antibody manipulation before reading this book. The objectives of the book are somewhat met. The authors do not specifically describe for whom this book is meant, but one would assume it is for those involved with antibody research. I believe the book is geared toward someone who has a great deal of familiarity and experience in the field of immunology and molecular biology. The book contains many well-written procedures and details, but could only be of use to a seasoned researcher in the antibody engineering field. The editor has brought together many different authors from around the world. Some chapters are very clear and easily understood, whereas others use too much jargon, leaving the text unclear and at times confusing. The book contains too few pertinent and high-quality illustrations. More and clearer illustrations would have made the book easier to follow. The references are current and are pertinent. The table of contents is laid out very well, and the book has a fair index that could have been expanded. A glossary of terms would have been very helpful. A listing of reagent manufacturers used in the protocols would also be of use. Readers will findthe step-by-step protocols, the notes, and hints at the end of the chapters of value. The overall appearance of the book is appealing, and the paperback format avails itself for laboratory use. This book is fairly written by a number of experienced authorities from around the world. Researchers who are deeply involved in the immunology and molecular biology field will find this book a welcome addition to the laboratory. University medical centers as well as other academic libraries should carry this book.

Mark A. Jandreski

A comprehensive collection of recently developed methods for producing new antibody reagents by immunization and recombinant DNA techniques, containing ready-to-use protocols that illuminate current areas of research on antibody structure, functions, and applications. The methods can be applied in basic immunological studies involving antibody specificity, catalysis, and evolution, and in the isolation of rare antibodies by phage display technology and the engineering of new antibodies by mutagenesis. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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