Apoptosis Techniques and Protocols

by Andréa C. LeBlanc (Editor)

Overview

The study of the apoptotic process has grown exponentially since the publication of the first edition of Apoptosis Techniques and Prools for the Neuromethods series in 1997. In this greatly updated second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cyhrome-c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of proapoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays. Written by investigators who have used the techniques extensively, each prool includes tips on avoiding pitfalls, notes on the method's advantages and disadvantages, and a critical survey of the literature.

Cutting-edge and highly practical, Apoptosis Techniques and Prools, Second Edition, offers both novice and seasoned investigators a rich panoply of the productive tools they need to unravel the molecular mechanisms of neuronal cell death.

Product Details

ISBN-13:	9781617373060
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/09/2010
Series:	Neuromethods , #37
Edition description:	Second Edition 2002
Pages:	250
Product dimensions:	5.98(w) x 9.02(h) x 0.24(d)

Table of Contents

Molecular Characterization of the Proapoptotic Protein Bax.- Assessing Cyhrome-c Release from Mihondria.- Monitoring Caspases in Neuronal Cell Death.- HSV Amplicon Vectors in Neuronal Apoptosis Studies.- Microinjections to Study the Specific Role of Proapoptotic Proteins in Neurons.- Designing Microarray Experiments for Neurobiology.- Analysis of Gene Expression by Nylon Membrane cDNA Arrays.- Glyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic Drugs.- Analytical Approaches for Investigating Apoptosis and Other Biochemical Events in Compartmented Cultures of Sympathetic Neurons.- Detection and Analysis of Synaptosis.- In Situ Detection of Apoptotic Neurons.- Apoptotic and Oxidative Indicators in Alzheimer’s Disease.

Editorial Reviews

Review of the first edition:

"The book is useful for an actively working neuroscientist who is going to study apotosis and has enough experience to choose the most suitable models and prools among all the diverse offerings of the book." Three Stars. -Doody's Health Sciences Book Review Journal

"...the book has updated many of the state-of-the-art techniques for analysis of neuronal apoptosis. More importantly, the book also offers the first-hand and step-by-step experimental details for molecular, cellular, biochemical and immunohishemical assays of neuronal apoptotic death." - Biotechnic & Hishemistry

From the Publisher

In this updated second edition, experts in chemistry, cell biology, neurology, and biopharmaceuticals describe techniques for studying neuronal cell death in step-by-step detail. These reproducible methods solve research problems surrounding the detection of key proteins involved in neuronal apoptosis, the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of pro-apoptotic proteins . There are also methods presented for the study of apoptosis mechanisms in neuronal compartments, for studying synapsosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays. Each protocol includes tips on avoiding pitfalls and notes on advantages and disadvantages. LeBlanc is affiliated with McGill University. Annotation c. Book News, Inc., Portland, OR (booknews.com)