Basic Methods for the Biochemical Lab

by Martin Holtzhauer

Overview

Basic Methods for the Biochemical Lab elucidates proven lab procedures and practical hints for research in analytical and preparative biochemistry, as well as summarizing key data in numerous tables. To further emphasize the practical aspects and to minimize the text, theoretical introductions into the methods are mostly omitted.

The chapters cover: quantitative methods (proteins, nucleic acids, phospholipids and carbohydrates), electrophoresis (several polyacrylamide and agarose systems, 2D-PAGE, detection methods and affinity electrophoresis), chromatographic prools (thin-layer chromatography, GPC, IEC, affinity chromatography, HPLC), immunochemical prools (hapten-carrier and enzyme conjugation, immunization and antibody purification, immune affinity chromatography, ELISA), centrifugation (differential and density gradient centrifugation for cells and cellular fractions), radioactivity (labeling and counting), buffers (buffer properties and compositions).

In additional chapters, tables compile a broad array of useful information, e.g. SI units conversion factor, detergent, protein and nucleotide data, and the basic principles of statistics and enzyme and receptor kinetics are reviewed.

This first English-language edition of a successful German-language manual will be a valuable resource for students and experts in biochemistry, biotechnology and biomedical laboratories.

Product Details

ISBN-13:	9783540327851
Publisher:	Springer Berlin Heidelberg
Publication date:	08/29/2006
Series:	Springer Lab Manuals
Edition description:	2006
Pages:	252
Product dimensions:	6.10(w) x 9.25(h) x (d)

Table of Contents

Quantitative Methods.- Electrophoresis.- Chromatography.- Immunochemical Prools.- Centrifugation.- Radioactive Labeling.- Buffers.- Tables.- Statistics and Data Analysis.