Biobanking: Methods and Protocols

by William H. Yong (Editor)

Overview

This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research. Other chapters in this book span practical topics including: disaster prevention and recovery; information technology; flora and fauna preservation including zoological fluid specimen photography; surgical and autopsy biobanking; biobanking of bodily fluids; biosafety; cutting frozen sections; immunohishemistry; nucleic acid extraction; and biospecimen shipping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Unique and comprehensive, Biobanking: Methods andProols is a valuable resource for novice and practicing biobankers, and for end-user researchers. This book aims to bring new insight into the field and expand on current biomedical biobanking studies.

Product Details

ISBN-13:	9781493989331
Publisher:	Springer New York
Publication date:	12/12/2018
Series:	Methods in Molecular Biology , #1897
Edition description:	1st ed. 2019
Pages:	448
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Sustainability in Biobanking- An Introduction to Starting a Biobank.- An Introduction to Hardware, Software, and Other Information Technology Needs of Biomedical Biobanks.- Disaster Prevention and Recovery.- Minority Participation in Biobanks: An Essential Key to Progress.- Orientation and Training of New Biobank Personnel.- Procurement and Storage of Surgical Biospecimens.- Autopsy Biobanking: Biospecimen Procurement, Integrity, Storage, and Utilization.- Procurement, Storage, and Use of Blood in Biobanks.- Procurement, Transportation, and Storage of Saliva, Buccal Swab, and Oral Wash Specimens.- Biobanking of Cerebrospinal Fluid.- Biobanking of Urine Samples.- Procurement and Storage of Pleural and Peritoneal Fluids for Biobanking.- The Fluid Preservation of Zoological Specimens.- Photographing Fluid Preserved Specimens.- Casts of Fluid Preserved Specimens.- Collection and Preservation of Terrestrial Arthropods.- Procurement and Preservation of Plants.- Biosafety and Biohazards: Understanding Biosafety Levels and Meeting Safety Requirements of a Biobank.- Decontamination of Biobank Facilities.- Laboratory Safety: Chemical and Physical Hazards.- Making Formalin-Fixed Paraffin Embedded Blocks.- Microtomy: Cutting Formalin-Fixed Paraffin Embedded Sections.- Performing and Cutting Frozen Sections.- An Introduction to the Performance of Immunohishemistry.- An Introduction to Performing Immunofluorescence Staining.- Making a Tissue Microarray.- Nucleic Acid Isolation and Quality Control.- Fundamentals of RNA Analysis on Biobanked Specimens.- Nucleic Acid Extraction from Human Biological Samples.- An Overview of DNA Analytical Methods.- Shotgun Proteomic Profiling of Blood Borne Nanoscale Extracellular Vesicles (NsEVs).- Sample Preparation for Transmission Electron Microscopy.- Processing of Primary Patient Tumors and Subsequent Generation of Primary Cell Lines.- Domestic and International Shipping of Biospecimens.