Biochemistry / Edition 4

by Donald Voet, Judith G. Voet

Overview

Since its first edition in 1990, over 250,000 students have used Biochemistry by Donald Voet of the University of Pennsylvania and Judith Voet of Swarthmore College. It has been translated into six languages (Japanese, German, French, Spanish, Italian, and Czech) and is regarded around the world as the standard in its field.

Product Details

ISBN-13:	9780470570951
Publisher:	Wiley
Publication date:	12/01/2010
Edition description:	4th ed.
Pages:	1520
Sales rank:	701,117
Product dimensions:	8.90(w) x 11.10(h) x 2.20(d)

About the Author

Donald Voet received a Ph.D. in chemistry from Harvard University and did postdoctoral research in the Biology Department at MIT. Don took up a faculty position in the department of chemistry at the University of Pennsylvania, upon completing his postdoctoral work. He has been a visiting scholar at Oxford University, the University of California San Diego, and the Weismann Institute of Science in Israel.

Judith Voet received her Ph.D. from Brandeis University and did postdoctoral research at the University of Pennsylvania, Haverford College and the Fox  Chase Cancer Center. She taught at the University of Delaware before moving to Swarthmore College. Together with Don Voet, she is Co-Editor-in-Chief of the journal Biochemistry and Molecular Biology Education.

Table of Contents

Part I	Introduction and Background	1
Chapter 1	Life	3
1.	Prokaryotes	3
2.	Eukaryotes	7
3.	Biochemistry: A Prologue	14
4.	Genetics: A Review	19
5.	The Origin of Life	28
6.	The Biochemical Literature	34
Chapter 2	Aqueous Solutions	39
1.	Properties of Water	39
2.	Acids, Bases, and Buffers	44
Chapter 3	Thermodynamic Principles: A Review	51
1.	First Law of Thermodynamics: Energy Is Conserved	52
2.	Second Law of Thermodynamics: The Universe Tends toward Maximum Disorder	53
3.	Free Energy: The Indicator of Spontaneity	56
4.	Chemical Equilibria	57
Appendix	Concentration Dependence of Free Energy	60
Part II	Biomolecules	63
Chapter 4	Amino Acids	65
1.	The Amino Acids of Proteins	65
2.	Optical Activity	71
3.	"Nonstandard" Amino Acids	76
Chapter 5	Nucleic Acids, Gene Expression, and Recombinant DNA Technology	80
1.	Nucleotides and Nucleic Acids	80
2.	DNA Is the Carrier of Genetic Information	82
3.	Double Helical DNA	85
4.	Gene Expression and Replication: An Overview	92
5.	Molecular Cloning	101
Chapter 6	Techniques of Protein and Nucleic Acid Purification	127
1.	Protein Isolation	127
2.	Solubilities of Proteins	131
3.	Chromatographic Separations	133
4.	Electrophoresis	144
5.	Ultracentrifugation	151
6.	Nucleic Acid Fractionation	155
Chapter 7	Covalent Structures of Proteins and Nucleic Acids	161
1.	Primary Structure Determination of Proteins	162
2.	Nucleic Acid Sequencing	175
3.	Chemical Evolution	182
4.	Bioinformatics: An Introduction	191
5.	Chemical Synthesis of Polypeptides	203
6.	Chemical Synthesis of Oligonucleotides	207
Chapter 8	Three-Dimensional Structures of Proteins	219
1.	Secondary Structure	219
2.	Fibrous Proteins	231
3.	Globular Proteins	240
4.	Protein Stability	258
5.	Quaternary Structure	265
Appendix	Viewing Stereo Pictures	269
Chapter 9	Protein Folding, Dynamics, and Structural Evolution	276
1.	Protein Folding: Theory and Experiment	276
2.	Folding Accessory Proteins	288
3.	Protein Structure Prediction and Design	299
4.	Protein Dynamics	302
5.	Conformational Diseases: Amyloids and Prions	306
6.	Structural Evolution	312
Chapter 10	Hemoglobin: Protein Function in Microcosm	320
1.	Hemoglobin Function	320
2.	Structure and Mechanism	327
3.	Abnormal Hemoglobins	339
4.	Allosteric Regulation	345
Appendix	Derivation of Symmetry Model Equations	352
Chapter 11	Sugars and Polysaccharides	356
1.	Monosaccharides	356
2.	Polysaccharides	362
3.	Glycoproteins	369
Chapter 12	Lipids and Membranes	382
1.	Lipid Classification	382
2.	Properties of Lipid Aggregates	389
3.	Biological Membranes	394
4.	Membrane Assembly and protein Targeting	414
5.	Lipoproteins	439
Part III	Mechanisms of Enzyme Action	457
Chapter 13	Introduction to Enzymes	459
1.	Historical Perspective	459
2.	Substrate Specificity	460
3.	Coenzymes	463
4.	Regulation of Enzymatic Activity	465
5.	A Primer of Enzyme Nomenclature	470
Chapter 14	Rates of Enzymatic Reactions	472
1.	Chemical Kinetics	473
2.	Enzyme Kinetics	477
3.	Inhibition	482
4.	Effects of pH	486
5.	Bisubstrate Reactions	487
Appendix	Derivations of Michaelis-Menten Equation Variants	491
Chapter 15	Enzymatic Catalysis	496
1.	Catalytic Mechanisms	496
2.	Lysozyme	507
3.	Serine Proteases	515
4.	Drug Design	528
Part IV	Metabolism	547
Chapter 16	Introduction to Metabolism	549
1.	Metabolic Pathways	549
2.	Organic Reaction Mechanisms	552
3.	Experimental Approaches to the Study of Metabolism	559
4.	Thermodynamics of Phosphate Compounds	566
5.	Oxidation-Reduction Reactions	571
6.	Thermodynamics of Life	574
Chapter 17	Glycolysis	581
1.	The Glycolytic Pathway	582
2.	The Reactions of Glycolysis	585
3.	Fermentation: The Anaerobic Fate of Pyruvate	602
4.	Metabolic Regulation and Control	607
5.	Metabolism of Hexoses Other than Glucose	618
Chapter 18	Glycogen Metabolism	626
1.	Glycogen Breakdown	626
2.	Glycogen Synthesis	632
3.	Control of Glycogen Metabolism	635
4.	Glycogen Storage Diseases	651
Chapter 19	Signal Transduction	657
1.	Hormones	657
2.	Heterotrimeric G Proteins	673
3.	Tyrosine Kinase-Based Signaling	683
4.	The Phosphoinositide Cascade	707
Chapter 20	Transport through Membranes	726
1.	Thermodynamics of Transport	726
2.	Kinetics and Mechanisms of Transport	727
3.	ATP-Driven Active Transport	738
4.	Ion Gradient-Driven Active Transport	746
5.	Neurotransmission	750
Chapter 21	Citric Acid Cycle	765
1.	Cycle Overview	765
2.	Metabolic Sources of Acetyl-Coenzyme A	768
3.	Enzymes of the Citric Acid Cycle	781
4.	Regulation of the Citric Acid Cycle	790
5.	The Amphibolic Nature of the Citric Acid Cycle	792
Chapter 22	Electron Transport and Oxidative Phosphorylation	797
1.	The Mitochondrion	798
2.	Electron Transport	802
3.	Oxidative Phosphorylation	820
4.	Control of ATP Production	836
Chapter 23	Other Pathways of Carbohydrate Metabolism	843
1.	Gluconeogenesis	843
2.	The Glyoxylate Cycle	850
3.	Biosynthesis of Oligosaccharides and Glycoproteins	852
4.	The Pentose Phosphate Pathway	862
Chapter 24	Photosynthesis	871
1.	Chloroplasts	871
2.	Light Reactions	873
3.	Dark Reactions	896
Chapter 25	Lipid Metabolism	909
1.	Lipid Digestion, Absorption, and Transport	909
2.	Fatty Acid Oxidation	914
3.	Ketone Bodies	928
4.	Fatty Acid Biosynthesis	930
5.	Regulation of Fatty Acid Metabolism	940
6.	Cholesterol Metabolism	942
7.	Eicosanoid Metabolism: Prostaglandins, Prostacyclins, Thromboxanes, Leukotrienes, and Lipoxins	959
8.	Phospholipid and Glycolipid Metabolism	969
Chapter 26	Amino Acid Metabolism	985
1.	Amino Acid Deamination	985
2.	The Urea Cycle	991
3.	Metabolic Breakdown of Individual Amino Acids	995
4.	Amino Acids as Biosynthetic Precursors	1013
5.	Amino Acid Biosynthesis	1030
6.	Nitrogen Fixation	1044
Chapter 27	Energy Metabolism: Integration and Organ Specialization	1054
1.	Major Pathways and Strategies of Energy Metabolism: A Summary	1054
2.	Organ Specialization	1057
3.	Metabolic Homeostasis: Regulation of Appetite, Energy Expenditure, and Body Weight	1061
4.	Metabolic Adaptation	1065
Chapter 28	Nucleotide Metabolism	1069
1.	Synthesis of Purine Ribonucleotides	1069
2.	Synthesis of Pyrimidine Ribonucleotides	1076
3.	Formation of Deoxyribonucleotides	1080
4.	Nucleotide Degradation	1092
5.	Biosynthesis of Nucleotide Coenzymes	1098
Part V	Expression and Transmission of Genetic Information	1105
Chapter 29	Nucleic Acid Structures	1107
1.	Double Helical Structures	1107
2.	Forces Stabilizing Nucleic Acid Structures	1115
3.	Supercoiled DNA	1122
Chapter 30	DNA Replication, Repair, and Recombination	1136
1.	DNA Replication: An Overview	1136
2.	Enzymes of Replication	1139
3.	Prokaryotic Replication	1152
4.	Eukaryotic Replication	1162
5.	Repair of DNA	1173
6.	Recombination and Mobile Genetic Elements	1184
7.	DNA Methylation and Trinucleotide Repeat Expansions	1204
Chapter 31	Transcription	1216
1.	The Role of RNA in Protein Synthesis	1216
2.	RNA Polymerase	1221
3.	Control of Transcription in Prokaryotes	1237
4.	Posttranscriptional Processing	1254
Chapter 32	Translation	1285
1.	The Genetic Code	1285
2.	Transfer RNA and Its Aminoacylation	1292
3.	Ribosomes and Polypeptide Synthesis	1309
4.	Control of Eukaryotic Translation	1342
5.	Posttranslational Modification	1347
6.	Protein Degradation	1352
Chapter 33	Viruses: Paradigms for Cellular Function	1372
1.	Tobacco Mosaic Virus	1374
2.	Icosahedral Viruses	1379
3.	Bacteriophage [lambda]	1390
4.	Influenza Virus	1408
Chapter 34	Eukaryotic Gene Expression	1422
1.	Chromosome Structure	1422
2.	Genomic Organization	1432
3.	Control of Expression	1446
4.	Cell Differentiation and Growth	1482
Chapter 35	Molecular Physiology
1.	Blood Clotting
2.	Immunity
3.	Motility: Muscles, Cilia, and Flagella
	Index	1527

Editorial Reviews

An opulent and expansive comprehensive textbook for students who have had the equivalent of one year of college chemistry and at least one semester of organic chemistry, as well as a one-year college course in general biology in which elementary biochemical concepts were discussed. This edition is updated throughout to keep pace with recent research advances, particularly in nucleic acid and protein structure. It also features an increased emphasis on human disease, more extensive use of molecular biological techniques, and striking new molecular graphics illustrations. Annual supplements are available on a subscription basis. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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