Cell Cycle Oscillators: Methods and Protocols

by Amanda S. Coutts (Editor), Louise Weston (Editor)

Overview

This book brings together a unique collection of protocols that cover novel and specialized techniques as well as updated and improved adaptations of more standard procedures involving the cell cycle and its regulation by oscillatory networks, exploring recent progress in the field from both holistic and reductionist perspectives. The edition provides a space for researchers to highlight and explore the latest developments in molecular biology and biochemical techniques for studying oscillatory networks and to share these across the research community to facilitate further progress. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Cell Cycle Oscillators: Methods and Protocols, Second Edition serves as an invaluable guide for those new to the field as well as the more experienced scientist.

Chapters 19 and 21 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Product Details

ISBN-13:	9781071615409
Publisher:	Springer US
Publication date:	06/05/2022
Series:	Methods in Molecular Biology , #2329
Edition description:	Second Edition 2021
Pages:	340
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Cell Cycle Control: A System of Interlinking Oscillators.- Model Organisms for Studying the Cell Cycle.- Role of Computational Modelling in Understanding Cell Cycle Oscillators.- E2F Transcription Factors Control the Roller Coaster Ride of Cell Cycle Gene Expression.- Cell Synchronization of Mouse Embryonic Fibroblasts.- Cell Cycle Synchronisation in Xenopus Egg Extracts.- Elutriation for Cell Cycle Synchronization in Fission Yeast.- Spatiotemporal Investigation of Phosphorylation Events during Cell Cycle Progression.- Cell Cycle Dynamics of Proteins and Post-translational Modifications Using Quantitative Immunofluorescence.- Building a Synthetic Transcriptional Oscillator.- Time Series Simulations and Bifurcation Analyses of a Model of Mitotic Oscillations in Xenopus Cell-free Extracts.- A Computational Method for Identifying Yeast Cell Cycle Transcription Factors.- Measuring Activity and Specificity of Protein Phosphatases.- Combining the Optimized Yeast Cytosine Deaminase Protein Fragment Complementation Assay and an in vitro Cdk1 Targeting Assay to Study the Regulation of the g-Tubulin Complex.- Cell Cycle Synchronisation Using a Microfluidic Synchroniser for Fission Yeast Cells.- Detection of Protein-Protein Interactions in Tobacco BY-2 cells Using BimolecularFluorescence Complementation.- Tracking the Cyclin B1-GFP Sensor to Profile the Pattern of Mitosis Versus Mitotic Bypass.- Measuring APC/C Dependent Ubiquitylation in vitro.- Using the Fly-FUCCI System for the Live Analysis of Cell Cycle Dynamics in Cultured Drosophila cells.- Imaging Cell Cycle Phases and Transitions of LivingCells From Yeast to Woman.- Measurement of Cdk1/cyclin B Kinase Activity by Specific Antibodies and Western Blotting.- The use of SNAP Labeling to Study Cell Cycle Oscillatory Proteins.