Comparative Biology of Aging / Edition 1

by Norman S. Wolf

Overview

determined by an inability to move in response to touch. C. elegans develop through four larval stages following hatching and prior to adulthood. Adult C. elegans are reproductive for about the first week of adulthood followed by approximately two weeks of post-reproductive adulthood prior to death. Life span is most commonly measured in the laboratory by maintaining the worms on the surface of a nutrie- agar medium (Nematode Growth Medium, NGM) with E. coli OP50 as the bacterial food source (REF). Alternative culture conditions have been described in liquid media; however, these are not widely used for longevity studies. Longevity of the commonly used wild type C. elegans hermaphrodite (N2) varies— from 16 to 23 days under standard laboratory conditions (20 C, NGM agar, E. coli OP50 food source). Life span can be increased by maintaining animals at lower ambient temperatures and shortened by raising the ambient temperature. Use of a killed bacterial food source, rather than live E. coli, increases lifespan by 2–4 days, and growth of adult animals in the absence of bacteria (axenic growth or bac- rial deprivation) increases median life span to 32–38 days [3, 23, 24]. Under both standard laboratory conditions and bacterial deprivation conditions, wild-derived C. elegans hermaphrodites exhibit longevity comparable to N2 animals [25].

Product Details

ISBN-13:	9789048134649
Publisher:	Springer Netherlands
Publication date:	02/01/2010
Edition description:	2010
Pages:	391
Product dimensions:	6.10(w) x 9.20(h) x 1.10(d)

Table of Contents

Introduction: Lifespans and Pathologies Present at Death in Laboratory Animals Norman S. Wolf Steven Austad 1

Animal Size, Metabolic Rate, and Survival, Among and Within Species Steven N. Austad 27

Hormonal Influences on Aging and Lifespan Adam Spong Andrzej Bartke 43

Exploring Mechanisms of Aging Retardation by Caloric Restriction: Studies in Model Organisms and Mammals Rozalyn M. Anderson Ricki J. Colman Richard Weindruch 69

Cell Replication Rates In Vivo and In Vitro and Wound Healing as Affected by Animal Age, Diet, and Species Norman S. Wolf 97

Sirtuin Function in Longevity Daniel L. Smith Jr. Jeffrey S. Smith 123

The Role of TOR Signaling in Aging Matt Kaeberlein Lara S. Shamieh 147

Mitochondria, Oxidative Damage and Longevity: What Can Comparative Biology Teach Us? Yun Shi, Rochelle Buffenstein Holly Van Remmen 163

Comparative Genomics of Aging Jan Vijg, Ana Maria Garcia, Brent Calder, and Martijn Dollé 191

Changes in Lysosomes and Their Autophagic Function in Aging: The Comparative Biology of Lysosomal Function Samantha J. Orenstein Ana Maria Cuervo 201

Telomeres and Telomerase N.M.V. Gomes J.W. Shay W.E. Wright 227

Cardiac Aging Dao-Fu Dai Robert J. Wessells Rolf Bodmer Peter S. Rabinovitch 259

Comparative Skeletal Muscle Aging David J. Marcinek Jonathan Wanagat Jason J. Villarin 287

Aging of the Nervous System Catherine A. Wolkow Sige Zou Mark P. Mattson 319

Aging of the Immune System Across Different Species Janko Nikolich-Žugich Luka Čičin-Šain 353

Index 377