Confocal Microscopy

by Jian Liu

Overview

The confocal microscope is appropriate for imaging cells or the measurement of industrial artefacts. However, junior researchers and instrument users sometimes misuse imaging concepts and metrological characteristics, such as position resolution in industrial metrology and scale resolution in bio-imaging. And, metrological characteristics or influence factors in 3D measurement such as height assessment error caused by 3D coupling effect are so far not yet identified. In this book, the authors outline their practices by the working experiences on standardization and system design. This book assumes little previous knowledge of optics, but rich experience in engineering of industrial measurements, in particular with profile metrology or areal surface topography will be very helpful to understand the theoretical concerns and value of the technological advances. It should be useful for graduate students or researchers as extended reading material, as well as microscope users alongside their handbook.

Product Details

ISBN-13:	9781643278889
Publisher:	Morgan and Claypool Publishers
Publication date:	12/01/2016
Series:	Iop Concise Physics
Pages:	90
Product dimensions:	7.00(w) x 0.00(h) x 0.00(d)

About the Author

Jian Liu is Professor and vice dean of the School of Electrical Engineering and Automation, Harbin Institute of Technology, China, Honorary Professor at the University of Nottingham, UK. His academic interests lie in the theories and implementations of optical microscopes, in particular the development of confocal microscopes, applied optics and optical metrology. He is also council member of China Optical Society for Engineering and China Instrument and Control Society, and also a member of ISO/TC213 and China SAC/TC240, both for geometrical products specifications, and a board member of Journal of Microscopy, Surface Topography: Metrology & Properties and Optics Communications.

Table of Contents

Table of Contents: Preface / Acknowledgments / Author biography / Confocal microscopy and its application in China / Point spread function model / Incoherent three-dimensional optical transfer function / / Decoupling criteria for three-dimensional optical microscopic measurement / Pupil filter design / Confocal axial peak extraction algorithm / Differential confocal microscopy / Medium aided scattering measurement / Scanning technology / Confocal profilometer