Cytochrome Systems: Molecular Biology and Bioenergetics

by S. Papa, B. Chance, L. Ernster

Overview

This volume is based on the proceedings of an International Symposium on "Cyhrome Systems: Molecular Biology and Bioenergetics" that was held at Selva di Fasano near Bari, Italy, between April 7 and 11,1987. It contains papers covering the subjects discussed at the Symposium, contributed both by participants of the meeting and by some invited speakers who were not able to attend. The aim of the Symposium was to bring together experts in various rese­ arch strategies currently being applied to the study of cyhrome systems, including molecular genetics, protein chemistry, enzymology of electron transfer and protonmotive activity in energy-transducing biological mem­ branes. Because of the high degree of complexity of cyhrome systems and the increasing sophistication in recent years of the different experimen­ tal approaches, there has been a growing specialization - sometimes even a tendency to "over-specialization" - among scientists working in this field. This in itself seemed to justify a meeting where representatives of various disciplines could exchange their results and discuss their conclusions. In addition, and perhaps even more importantly, it was felt that meetings of this kind provide an opportunity for a "cross-fertilization" of approaches and ideas among representatives of various fields of science. The present meeting proved to be an ample illustration of the success of such an inter­ action.

Product Details

ISBN-13:	9781461290780
Publisher:	Springer US
Publication date:	12/12/2012
Edition description:	Softcover reprint of the original 1st ed. 1987
Pages:	822
Product dimensions:	7.01(w) x 10.00(h) x 0.06(d)

Table of Contents

Cyhrome Systems: from Discovery to Present Developments.- I. Genetics.- Genetics of E.Coli Cyhromes which are Components of the Aerobic Respiratory Chain.- Molecular Genetic Approaches to Studying the Structure and Function of the Cyhrome c2 and the Cyhrome bc1 Complex from Rhodobacter Capsulatus.- Structural Homologies in the Cyhrome B/C1 Complex from Rhodobacter.- Genes and Sequences for Subunits of Respiratory Complexes in Paracoccus denitrificans.- Organization and Expression of Nuclear Genes for Yeast Cyhrome c Oxidase.- The cDNA a Structural Gene for Rat Liver Cyhrome c Oxidase Subunit Vic.- Genetics of Yeast Coenzyme QH2-Cyhrome c Reductase.- Sequence Analysis of Mammalian Cyhrome b Mutants.- Plant Mihondrial Genes, Cyhrome c Oxidase and Cytoplasmic Male Sterility.- A Mutation Affecting Lariat Formation, but not Splicing of a Yeast Mihondrial Group II Intron.- The Evolution of Mihondrially Coded Cyhrome Genes: A Quantitative Estimate.- Involvement of Nuclear Genes in Splicing of the Mihondrial COB Transcript in S. Cerevisiae.- Introns as Key Elements in the Evolution of Mihondrial Genomes in Lower Eukaryotes.- GENETICS: Short Reports.- Core Protein Deficiency in Complex III of the Respiratory Chain, in a Missence Exonic Mihondrial Yeast Mutant of Cyhrome b Gene.- Localization and Partial Sequencing Analysis of Mihondrial Gene for Apocyhrome b in Sunflower.- Molecular Basis for Resistance to Inhibitors of the Mihondrial Ubiquinol-Cyhromec Reductase in Saccharmoyces Cerevisiae.- Structure of the Proteins and Genes for the Nuclear-Encoded Subunits of N. Crassa Cyhrome Oxidase.- Partial Sequence of Gene for Cyhrome Oxydase Subunit II of Sunflower Mihondrial DNA.- Unusual Features of Kinetoplast DNA from Trypanosomes: Novel Mechanism of Gene Expression (RNA-Editing), the Absence of tRNA Genes and the Variable Presence of a Cyhrome Oxidase Subunit Gene.- II. Biosynthesis.- Heterogeneous Efficiencies of mRNA Translation in Human Mihondria.- Regulation of the Expression of COI and COIII mRNAs in Rat Liver Mihondria.- Two Consensus Sequences for RNA Processing in Neurospora Crassa Mihondria.- Primary Structure of the Yeast Nuclear CBS 1 Gene Product, Necessary to Activate Translation of Mihondrial COB mRNA.- A Calmodulin-Like Protein in the Cyhrome bc1 Complex Required for Synthesis of Both Cyhrome bc1 and Cyhrome c Oxidase Complexes in Yeast Mihondria.- Import of Cyhromes b2 and c1 into Mihondria is Dependent on Both Membrane Potential and Nucleoside Triphosphates.- NADH: A Common Requirement for the Import and Maturation of Cyhromes c and c1.- Import of Proteins into Mihondria: Structural and Functional Role of the Prepeptide.- Interaction of the Mihondrial Precursor Protein Apocyhrome c with Model Membranes and its Implications for Protein Translocation.- Influence of Thyroid Hormones on the Expression of Nuclear Genes Encoding Subunits of the Cyhrome bc1 Complex.- II. BIOSYNTHESIS: Short Report.- Import of Cyhrome c1 and Cyhrome b2 into Mihondria.- III. Protein Structure.- Cyhrome c Oxidase: Past, Present and Future.- Universal Features in Cyhrome Oxidase.- Polypeptide Subunits Encoded by Nuclear Genes are Essential Components of Eukaryotic Cyhrome c Oxidase.- Respiratory Complex IV and Cyhrome a,a3.- An Amino Acid Sequence Region of Subunit II of Cyhrome Oxidase which may be Responsible for Evolutionary Changes in Reactivity with Different Cyhromes c.- Evidence for a Functional Role of Nuclear Encoded Subunits in Mihondrial Cyhrome c Oxidase.- Structural Analysis of the bc1 Complex from Beef Heart Mihondria by the Sided Hydropathy Plot and by Comparison with Other bc1 Complexes.- Crystallisation of Water-Soluble Preparations of the Iron- Sulfur-Protein and Cyhrome c1 of Ubiquinol: Cyhrome Reductase from NeurosporaMihondria.- Iron Cluster Sites of Cardiac Ip-Subunit of Succinate Dehydrogenase.- Polypeptides in Mihondrial Electron-Transfer Complexes.- The Fatty Acid-Anchored Four Heme Cyhrome of the Photo- synthetic Reaction Center from the Purple Bacterium Rhodopseudomonas Viridis.- III. PROTEIN STRUCTURE: Short Reports.- Membrane Protein in Paracoccus Denitrificans Resembles Subunit III of Beef Heart Cyhrome Oxidase.- The Circular Dichroism Properties of the Rieske Protein and the b and c1 Cyhromes of the Mihondrial bc1 Complex.- Monomerization of Cyhrome Oxidase Might Be Essential for Subunit III Removal.- Fourier-Transform Infra-Red Studies of Cyhrome c Oxidase.- Isoenzymes of Human Cyhrome c Oxidase.- Folding of Integral Membrane Proteins: Renaturation Experiments with Bacteriorhodospin Support a Two-Stage Mechanism.- IV. Reaction Domains and Oxido-Reduction Mechanisms.- The Nature of Ferryl, Fe(IV)=0, Haem in Peroxidases.- Polarization in Heme Proteins and Cyhrome c Oxidase.- Effects of Freezing on the Coordination State and Ligand Orientation in Hemoproteins.- Catalytic Mechanism of O2 Reduction by Cyhrome Oxidase.- The Electron-Transfer Reactions in and Substrate Binding to Cyhrome c Oxidase.- Control of Cyhrome Oxidase in Proteoliposomes: Flux and Stoichiometry.- Cyhrome c Oxidase Activity is Regulated by Nucleotides and Anions.- Cyhrome Oxidase and Neuromuscular Diseases.- Electron Transfer Between Cardiac Cyhrome c1 and c.- Heme Cluster Structures and Electron Transfer in Multiheme Cyhromes C3.- IV. REACTION DOMAINS AND OXIDO-REDUCTION MECHANISMS: Short Reports.- Electron Exchange Between Cyhromes a.- Pulsed, Resting and Peroxy Forms of Cyhrome Oxidase.- Effect of pH and Temperature on Electron Transfer in Mixed-Valence Cyhrome c Oxidase.- The pH-Dependence of the Reductional Levels of Cyhrome c and Cyhrome a During Turnover of Cyhrome c Oxidase.- V. Fe-S Centers.- Studies of Bacterial NADH-Ubiquinone (or Menaquinone) Oxidoreductase Systems.- Localized Valence States in Iron-Sulfur Clusters and Their Possible Relationship to the Ability of Iron-Sulfur Clusters to Catalyze Reactions Other Than Electron Transfer.- Probing the Fe/S Domain With EPR: Pandora’s BOX Ajar.- Aspects of Spin Coupling Between Even and Odd Electron Systems: Applications to Succinate: Q Reductase.- The Iron-Sulfur Clusters in Succinate Dehydrogenase.- Mechanisms of Electron Transfer in Succinate Dehydrogenase and Fumarate Reductase: Possible Functions for Iron-Sulphur Centre 2 and Cyhrome b.- VI. Quinone Binding Sites: Structure and Function.- Structural and Mechanistic Aspects of the Quinone Binding Sites of the bc Complexes.- QP-S-The Electron Acceptor of Succinate Dehydrogenase.- Characteristics of QP-C and Reconstitution of the QH2-Cyt c reductase.- The Kinetics of Oxidation of Cyhrome b are in Agreement with the Q-Cycle Hypothesis.- The Q-Reaction Domains of Mihondrial QH2: Cyhrome c Oxidoreductase.- EPR Studies of the Quinone Reaction Sites in Bacteria.- EPR Signal II in Photosystem II: Redox and Paramagnetic Interactions with the O2 Evolving Enzyme.- Studies on the Ubiquinone Pool in Mihondrial Electron Transfer.- VI. Quinone Binding Sites: Structure and Function: Short Reports.- Effects of Proteolytic Digestion and Chemical Modification of b-c Complex on the Fe-S Center and the Stable Ubisemi-quinone (SQc).- Kinetics of Ubiquinol Cyhrome c Reductase: Lack of a Diffusion-Limited Step with Short Chain Ubiquinols as Substrates.- VII. Specific Redox Systems.- Biochemistry and Molecular Biology of the Escherichia Coli Aerobic Respiratory Chain.- The Interplay Between Photosynthesis and Respiration in Facultative Anoxygenic Phototrophic Bacteria.- The Ubiquinol-Cyhrome c Oxidoreductase of Phototrophic Bacteria.- The Redox Reaction Between Cyhrome bH and the Semiquinone-Quinol Couple of Qc is Electrogenic in Ubiquinol-Cyt c2 Oxidoreductase.- Construction and Characterization of Monolayer Films of the Reaction Center Cyhrome-c Protein from Rhodopseudomonas Viridis.- Structure-Function Relationships in the Rhodopseudomonas Viridis Reaction Center Complex: Electrogenic Steps Contributing to—? Formation.- Catalytic Sites for Reduction and Oxidation of Quinones.- Studies of the Electrogenicity of the Reduction of Cyhrome b561 through the Antimycin-Sensitive Site of the Ubiquinol-Cyhrome c2 Oxidoreductase Complex of Rhodobacter Sphaeroides.- Mutants Affecting the Quinol Oxidation Site of Ubiquinol-Cyt c2 Oxidoreductase.- Cyhrome b558 of Bacillus Subtilis.- Mihondrial Cyhrome b560.- Isolation of the Photosystem Two Reaction Centre and the location and Function of Cyhrome.- VII. SPECIFIC REDOX SYSTEMS: Short Reports.- Effect of Antimycin on the Kinetics of Reduction of b and c1 Cyhromes in Single Turnover of the Mihondrial b-c1 Complex.- The b-Q Cycle, a new model for the Pathways of Electrons and Protons through the bc1 Complex I Action of Antimycin A at Center O.- Rapid Oxidation Kinetics of Quinol Cyhrome c Oxidoreductase: Quinone or Semiquinone Cycle?.- Electron Conduction Between b Cyhromes of the Mihondrial Respiratory Chain in the Presence of Antimycin plus Myxothiazol.- The Release of NAD Radical is the Limiting Step in the Redox Reaction Between Fe(III) Cyhrome c and NADH.- Properties of Cyhrome b558 in Complex II of Ascaris Muscle Mihondria and its Function in Anaerobic Respiration.- VIII. Cooperativity and Allosteric Transitions.- Pulsed and Resting Cyhrome Oxidase: an Update of Optical and Molecular Properties.- Is H2O2 Involved in Electron Transport Between Cyhromes a and a3 in Cyhrome Oxidase?.- Conformational Transitions of Cyhrome c Oxidase Induced by Partial Reduction.- Redox-Linked Conformational Changes in Cyhrome c Oxidase.- IX. Proton-Motive Activity.- Protonmotive Activity of the Cyhrome Chain of Mihondria: Models and Features.- The Mechanism of Electron Gating in Cyhrome c Oxidase.- Proton Pump Activity in Bacterial Cyhrome Oxidases.- Topography and Protonmotive Mechanism of Mihondrial Coupling Site 2.- The Q-Gated Proton Pump Model of Ubiquinol Cyhrome c Reductase: Electron and Proton Transfer Pathways and Role of Polypeptides.- Photosynthetic Control and ATP/Electron Ratio in Bacterial Photophosphorylation.- IX. PROTON-MOTIVE ACTIVITY: Short Report.- Protonmotive Activity of Mihondrial Cyhrome c Oxidase.- Proposal for a Novel Nomenclature for the Subunits of Cyhrome c Oxidase G. Buse, G.C.M. Steffens, R. Biewald, B. Bruch and S. Hensel.- Author Index.