| Basil Rathbone | Sherlock Holmes |
| Edmund Breon | Julian Emery |
| Richard Greene | Sir Henry Baskerville |
| Nigel Bruce | Dr. John H. Watson,Dr. Watson |
| Olaf Hytten | Simpson, gunsmith |
| Alan Mowbray | Maj. Duncan-Bleek / Col. Sebastian Moran |
| Aubrey Mather | Alistair |
| Dennis Hoey | Inspector Lestrade,Insp. Lestrade |
| Evelyn Ankers | Kitty,Naomi Drake |
| Gale Sondergaard | Andrea Spedding |
| Gerald Hamer | Potts/Tanner/Ramson |
| Hillary Brooke | Lydia Marlow,Sally Musgrave |
| Ida Lupino | Ann Brandon |
| Kaaren Verne | Charlotte Eberli |
| Marjorie Lord | Nancy Partridge |
| Morton Lowry | Sanford, Ship's Steward |
| Alan Marshal | Jerrold Hunter |
| Henry Daniell | Prof. Moriarty,William Easter |
| Lionel Atwill | James Mortimer, M.D.,Prof. Moriarty |
| Milburn Stone | Capt. Vickery |
| Patricia Cameron | Evelyn Clifford |
| Paul Cavanagh | Lord William Penrose,Simon Merrivale,Sir George Fenwick |
| Reginald Denny | Sir Evan Barham |
| Arthur Hohl | Emile Journet |
| Arthur Margetson | Dr. Sexton |
| E.E. Clive | Inspector Bristol |
| George Zucco | Richard Stanley |
| John Carradine | Barryman |
| Marjorie Riordan | Sheila Woodbury, Singer |
| Miles Mander | Giles Conover |
| Patricia Morison | Hilda Courtney |
| Renee Godfrey | Vivian Vedder |
| Thomas Gomez | R.F. Meade |
| Vernon P. Downing | Norman Locke |
| William Post | Dr. Franz Tobel |
| Harry Cording | Hamid |
| Mary Forbes | Lady Margaret Carstairs |
| Matthew Boulton | Inspector Gregson |
| Terry Kilburn | Billy |
| Wendy Barrie | Beryl Stapleton |
| Billy Bevan | Train Attendant |
| Eve Amber | Maude Fenwick |
| Holmes Herbert | Sir Reginald Dailey |
| Tom Dillon | Detective Thompson |
| Frederic Worlock | Onslow,Prof. William Kilbane |
| Mary Gordon | Mrs. Hudson |
| Carl Harbord | Inspector Hopkins |
| Leyland Hodgson | Conductor |
| Rudolph Anders | Braun |
| Tom Bryson | Williams |
Technical Credits
| Alfred L. Werker | Director |
| John Rawlins | Director |
| Roy William Neill | Director |
| Sidney Lanfield | Director |
