Electroporation Protocols: Preclinical and Clinical Gene Medicine

Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture, and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues, and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.

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Electroporation Protocols: Preclinical and Clinical Gene Medicine

Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture, and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues, and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.

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Electroporation Protocols: Preclinical and Clinical Gene Medicine

Electroporation Protocols: Preclinical and Clinical Gene Medicine

Electroporation Protocols: Preclinical and Clinical Gene Medicine

Electroporation Protocols: Preclinical and Clinical Gene Medicine

Hardcover(2nd ed. 2014)

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Overview

Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture, and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues, and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.


Product Details

ISBN-13: 9781461496311
Publisher: Springer New York
Publication date: 02/08/2014
Series: Methods in Molecular Biology , #1121
Edition description: 2nd ed. 2014
Pages: 349
Product dimensions: 7.01(w) x 10.00(h) x 0.04(d)

Table of Contents

Preface     v
Contributors     xiii
Basic Concepts of DNA Transfer via Electroporation
Application of Electroporation Gene Therapy: Past, Current, and Future   Lluis M. Mir     3
Mechanism by Which Electroporation Mediates DNA Migration and Entry into Cells and Targeted Tissues   Marie-Pierre Rols     19
Applicator and Electrode Design for In Vivo DNA Delivery by Electroporation   Dietmar Rabussay     35
Electrode Assemblies Used for Electroporation of Cultured Cells   Leda Raptis   Kevin L. Firth     61
Formulations for DNA Delivery via Electroporation In Vivo   Khursheed Anwer     77
Overview of Drug Delivery and Alternative Methods to Electroporation   Sek-Wen Hui     91
Nanoelectroporation: A First Look   Raji Sundararajan     109
In Vitro Targeted Gene Delivery via Electroporation
Nucleofection of Human Embryonic Stem Cells   Henrike Siemen   Lars Nolden   Stefanie Terstegge   Philipp Koch   Oliver Brustle     131
Delivery of Whole Tumor Lysate into Dendritic Cells for Cancer Vaccination   Linda N. Liu   Rama Shivakumar   Cornell Allen   Joseph C. Fratantoni     139
Delivery of Tumor-Antigen-Encoding mRNA into Dendritic Cellsfor Vaccination   Annelies Michiels   Sandra Tuyaerts   Aude Bonehill   Carlo Heirman   Jurgen Corthals   Kris Thielemans     155
Delivery of DNA into Natural Killer Cells for Immunotherapy   Kathrin Schoenberg   Hans-Ingo Trompeter   Markus Uhrberg     165
Electroporation of Adherent Cells In Situ for the Study of Signal Transduction and Gap Junctional Communication   Leda Raptis   Adina Vultur   Heather L. Brownell   Evangelia Tomai   Aikaterini Anagnostopoulou   Rozanne Arulanandam   Jun Cao   Kevin L. Firth     173
Delivery of DNA into Adipocytes within Adipose Tissue   James G. Granneman     191
In Vivo Targeted Gene Delivery via Electroporation
Delivery of DNA into Muscle for Treating Systemic Diseases: Advantages and Challenges   Capucine Trollet   Daniel Scherman   Pascal Bigey     199
Delivery of DNA into Skeletal Muscle in Large Animals   Patricia A. Brown   Amir S. Khan   Ruxandra Draghia-Akli     215
Delivery of DNA into Skin via Electroporation   Babu M. Medi   Jagdish Singh     225
Electroporation-Mediated Gene Delivery to the Lungs   Rui Zhou   James E. Norton    David A. Dean     233
Delivery of DNA into Bladder via Electroporation   Masaki Yoshida   Hitoshi Iwashita   Masayuki Otani   Koichi Masunaga   Akito Inadome     249
Analysis of Gene Function in the Retina   Takahiko Matsuda   Constance L. Cepko     259
Optical In Vivo Imaging of Electrically Mediated Delivery of siRNA into Muscle for Gene Function Analysis   Muriel Golzio   Justin Teissie     279
Electroporation of Adult Zebrafish   N. Madhusudhana Rao   K. Murali Rambabu   S. Harinarayana Rao     289
Treatment of Cancer via Electroporation Gene Therapy
Flow Electroporation with Pulsed Electric Fields for Purging Tumor Cells   Abie Craiu   David Scadden     301
Delivery of DNA into Tumors   Shulin Li     311
Intratumoral Bleomycin and IL-12 Electrochemogenetherapy for Treating Head and Neck Tumors in Dogs   Jeffry Cutrera   Marina Torrero   Keijiro Shiomitsu   Neal Mauldin   Shulin Li     319
Systemic IL-12 Gene Therapy for Treating Malignancy via Intramuscular Electroporation   Shiguo Zhu   Shulin Li     327
Treatment of SCCVII Tumors with Systemic Chemotherapy and Interleukin-12 Gene Therapy Combination    Marina Torrero   Shulin Li     339
Electroporation for Drug and Gene Delivery in the Clinic: Doctors Go Electric   Julie Gehl     351
IL-2 Plasmid Electroporation: From Preclinical Studies to Phase I Clinical Trial   Holly M. Horton   Peggy A. Lalor   Alain P. Rolland     361
Treatment of Other Diseases via Electroporation Gene Therapy
Factor IX Gene Therapy for Hemophilia   Jason G. Fewell     375
KGF-1 for Wound Healing in Animal Models   Guy P. Marti   Parsa Mohebi   Lixin Liu   Jiaai Wang   Tomoharu Miyashita   John W. Harmon     383
Hepatocyte Growth Factor Gene Therapy for Hypertension   Kazuo Komamura   Jun-ichi Miyazaki   Enyu Imai   Kunio Matsumoto   Toshikazu   Nakamura   Masatsugu Hori     393
Electroporation of Corrective Nucleic Acids (CNA) In Vivo to Promote Gene Correction in Dystrophic Muscle   Robert M.I. Kapsa   Sharon H.A. Wong   Anita F. Quigley     405
Gene Delivery to Dystrophic Muscle   Kim E. Wells   Jill McMahon   Helen Foster   Aurora Ferrer   Dominic J. Wells     421
Effect of Electroporation on Cardiac Electrophysiology   Vadim V. Fedorov   Vladimir P. Nikolski   Igor R. Efimov     433
Muscle and Fat Mass Modulation in Different Clinical Models   Ruxandra Draghia-Akli   Amir S. Khan     449
Applications of Electroporation for DNA Vaccination
DNA Vaccination for Prostate Cancer   Anna-Karin Roos   Alan King   Pavel Pisa     463
HER2/neu DNA Vaccination for Breast Tumors   Arianna Smorlesi   Francesca Papalini   Sara Pierpaoli   Mauro Provinciali     473
Electroporation-Mediated HBV DNA Vaccination in Primate Models   Yong-Gang Zhao   Yuhong Xu     487
Taking Electroporation-Based Delivery of DNA Vaccination into Humans: A Generic Clinical Protocol   Torunn Elisabeth Tjelle   Dietmar Rabussay   Christian Ottensmeier   Iacob Mathiesen   Rune Kjeken     497
Production of Monoclonal Antibody by DNA Immunization with Electroporation   Kaw Yan Chua   John D.A. Ramos   Nge Cheong     509
Index     521
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