Enzyme Immunoassays: From Concept to Product Development

by S.S. Deshpande

Overview

This unique reference provides a pragmatic approach to the development of successful commercial immunodiagnostic products based on enzyme im munoassay technology. Presenting both basic and applied principles, En zyme Immunoassays gathers information on all aspects of this process, from the initial conceptualization to the introduction of the product to the market.

Product Details

ISBN-13:	9780412056017
Publisher:	Springer US
Publication date:	06/30/1996
Edition description:	1996
Pages:	464
Product dimensions:	6.14(w) x 9.21(h) x 0.36(d)
Age Range:	18 Years

Table of Contents

I: Basic Considerations (Concepts).- 1 Introduction.- 2 Antibodies: Biochemistry, Structure, and Function.- 3 Antigen-Antibody Reactions.- 4 Conjugation Techniques.- 5 Antibody Production.- 6 Enzymes and Signal Amplification Systems.- 7 Separation and Solid-Phase Systems.- II: Product Development.- 8 Immunoassay Classification and Commercial Technologies.- 9 Assay Development, Evaluation, and Validation.- 10 Reagent Formulations and Shelf Life Evaluation.- 11 Data Analysis.- 12 Documentation, Registration, and Diagnostics Start-Ups.

Editorial Reviews

A unique reference text emphasizing practical information for the development of commercial immunodiagnostic products based on enzyme immunoassay technology. Deshpande (Senior Scientist, Idetek Inc.) presents the basic concepts of classification, structure and function of antibodies and enzymes in immunoassays, applying these to the industrial product development process. This process is clearly outlined including various protocols for successful product design, product development, reagent formatting and assay development , data processing, standardization, scale up, commercial manufacture, regulatory requirements, laboratory practices and ISO 9000 certification. Contain some 75 illustrations and 40 tables. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

‘... all those who read this book will be far richer in their knowledge of enzyme immunoassays and their application.'
Chemistry and Industry
‘...if you're looking for a book that describes immunoassay methods in general then this one has something to recommend it...if you are looking to go beyond the laboratory and develop commercial assays then this book is worth buying for its second half alone.'
Analyst

From the Publisher