Enzyme Kinetics and Mechanism / Edition 1

by Paul F. Cook, W. W. Cleland

Overview

Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure.

Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data.

Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference.

Product Details

ISBN-13:	9780815341406
Publisher:	Taylor & Francis
Publication date:	03/06/2007
Edition description:	New Edition
Pages:	428
Product dimensions:	6.88(w) x 9.69(h) x (d)

About the Author

Paul F. Cook is the Grace B. Kerr Centennial Professor of Chemistry and Biochemistry at the University of Oklahoma. His awards and achievements twice included the Alexander von Humboldt Research Fellowship and he was the recipient of the National Institutes of Health Research Career Development Award from 1983 to 1988. Professor Cook is Associate Editor of Protein and Peptide Letters and Executive Editor of Proteins and Proteomics.

W.W. Cleland is the M.J. Johnson Professor and Steenbock Professor of Chemical Science at the Enzyme Institute at University of Wisconsin-Madison. In the early 1960s, Cleland's landmark papers created a theoretical framework for the study of steady-state kinetics of multisubstrate enzymes and laid the foundation for all subsequent steady-state studies. He serves on the editorial boards of Journal of Biological Chemistry and of Biochemistry. Recognized by many as the "father of modern enzyme kinetics," Professor Cleland was elected to the National Academy of Sciences in 1985.

Table of Contents

1. Nomenclature
2. Introduction to Kinetics
3. Enzyme Assays
4. Derivation of Initial Velocity Rate Equations and Data Processing
5. Initial Velocity Studies in the Absence of Added Inhibitors
6. Initial Velocity Studies: Presence of Added Inhibitors
7. Pre-Steady State and Relaxation Kinetics
8. Isotopic Probes of Kinetic Mechanism
9. Isotope Effects as a Probe of Mechanism
10. pH Dependence of Kinetic Parameters and Isotope Effects
Appendix I. King and Altman Patterns and Distribution Equations
Appendix II. Rate Equations, Definitions of Kinetic and Inhibition Constants, Haldanes, Distribution Equations and Rate Constants for a Number of Multireactant Mechanisms

Editorial Reviews

Reviewer: Eugene A Davidson, PhD (Georgetown University School of Medicine)
Description: This definitive book on enzyme kinetics explores essentially every possible mechanism with full mathematical treatment.
Purpose: The goal is to provide a thorough and detailed analysis of enzyme kinetic behavior and the use of kinetic approaches to elucidate mechanisms. The objective is important and the solution provided by the authors complete.
Audience: The target audience includes any scientist needing to understand the kinetic behavior of an enzyme or wishing to use kinetics to study possible mechanisms of inhibitor action. The authors are leading authorities.
Features: At first glance, the heavily mathematical approaches may cause apprehension in some readers. Nevertheless, those wishing to understand their favorite enzyme will profit from this book. The treatment proceeds in logical fashion beginning with simple mechanisms (Michaelis-Menten) and moving to more complex. Isotope and pH effects are analyzed and there is a detailed discussion of inhibitors. General texts simply do not provide a comparable treatment of this area and the investigator may miss important aspects of a given enzyme's behavior. Since many drugs function as inhibitors of specific enzymatic reactions, a complete understanding of kinetics is a valuable asset in studying such interactions. There are ample references to both the primary literature and selected books.
Assessment: The leading practitioner of our time (Cleland) has produced a complete treatment of kinetic issues relating to enzymes. This is a valuable reference that also can serve an instructional role.

Doody's Review Service

"Enzyme Kinetics and Mechanism is an advanced text with meticulous details...[that] gives 'hands on' explanations to the target readership..."

Journal of the Higher Education Academy Physical Sciences Centre, June 2008

From the Publisher

Enzyme Kinetics and Mechanism is an advanced text with meticulous details...[that] gives 'hands on' explanations to the target readership . . .

Journal of the Higher Education Academy Physical Sciences Centre