Exosomes and Microvesicles: Methods and Protocols

by Andrew F Hill (Editor)

Overview

This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, Exosomes and Microvesicles: Methods and Prools is a valuable resource containing methodologies for anyone interested in researching EVs.

Product Details

ISBN-13:	9781493982844
Publisher:	Springer New York
Publication date:	07/07/2018
Series:	Methods in Molecular Biology , #1545
Edition description:	Softcover reprint of the original 1st ed. 2017
Pages:	228
Product dimensions:	7.01(w) x 10.00(h) x (d)

About the Author

Andrew Hill, Associate Professor/Principle Research Fellow, Department of Biochemistry and Molecular Biology, The University of Melbourne Bio21 Institute,
Email: [e-mail]

Table of Contents

Methods to Analyze EVs.- Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles.- Immuno-Characterization of Exosomes using Nanoparticle Tracking Analysis.- Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy.- Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR.- Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM™ System.- A Prool for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration.- Multiplexed Phenotyping of Extracellular Vesicles using Protein Microarray (EV Array).- Purification and Analysis of Exosomes Released by Mature Cortical Neurons following Synaptic Activation.- A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space.- Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission.- Isolation of Platelet-Derived Extracellular Vesicles.- Bioinformatics Tools for Extracellular Vesicles Research.- Preparation and Isolation of siRNA-Loaded Extracellular Vesicles.- Interaction of Extracellular Vesicles with Endothelial Cells under Physiological Flow Conditions.