Forensic DNA Typing Protocols / Edition 1

by Angel Carracedo

Overview

A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.

Product Details

ISBN-13:	9781617374623
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/10/2010
Series:	Methods in Molecular Biology , #297
Edition description:	Softcover reprint of hardcover 1st ed. 2005
Pages:	280
Product dimensions:	5.98(w) x 9.02(h) x 0.03(d)

Table of Contents

Forensic DNA-Typing Technologies: A Review
Angel Carracedo and Paula Sánchez-Diz

DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR
Silvano Köchl, Harald Niederstätter, and Walther Parson

A Real-Time PCR Protocol to Determine the Number of Amelogenine (X-Y) Gene Copies From Forensic DNA Samples
Antonio Alonso and Pablo Martín

Species Determination: The Role and Use of the Cytochrome b Gene
Adrian Linacre and James Chun-I Lee

Constructing STR Mulitplex Assays
John M. Butler

Y Chromosome STR Typing
Leonor Gusmão and Cíntia Alves

Using Online Databases for Developing SNP Markers of Forensic Interest
Christopher Phillips

SNP Typing in Forensic Genetics: A Review
Beatriz Sobrino and Angel Carracedo

Genotyping SNPs With the LightCycler
María Victoria Lareu and Clara Ruiz-Ponte

Universal Tag Arrays in Forensic SNP Analysis
Marie Allen and Anna-Maria Divne

SNP Typing on the NanoChip Electronic Microarray
Claus Børsting, Juan J. Sanchez, and Niels Morling

Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS
Peter M. Vallone, Kristina Fahr, and Markus Kostrzewa

Mitochondrial D-Loop and Coding Sequence Analysis Using Pyrosequencing
Marie Allen and Hanna Andréasson

SNaPshot Typing of Mitochondrial DNA Coding Region Variants
Antonio Salas, Beatriz Quintáns, and Vanesa Álvarez-Iglesias

Typing of Y Chromosome SNPs With Multiplex PCR Methods
Juan J. Sanchez, Claus Børsting, and Niels Morling

Y Chromosome SNP Analysis Using Single-Base Extension: A Hierarchical Multiplex Design
María Brión

SNaPshot for Pharmacogenetics by Minisequencing
Klaus Bender

Methods for the Study of Ancient DNA
Christine Keyser-Tracqui and BertrandLudes

Protocols for Ancient DNATyping
Cristian Capelli and Frank Tschentscher

Index