Handbook of Proteomic Methods

by P. Michael Conn (Editor)

Overview

A compendium of thirty-four powerful techniques for identifying and analyzing the diversity of proteins expressed in cells. Thee readily reproducible proteomic methods range from general to specific techniques, and include methods for data analysis, posttranslational modification, and its variants and isoforms. Additional methods demonstrate the application of proteomics to the discovery of serological tumor markers, to identifying the determinants of sensitivity to antitumor drugs, and to specialized fields, such as endocrinology, plant biology, nephrology, and urology.

Product Details

ISBN-13:	9781588293404
Publisher:	Springer-Verlag New York, LLC
Publication date:	07/10/2003
Edition description:	2003
Pages:	510
Product dimensions:	7.01(w) x 10.00(h) x 0.05(d)

Table of Contents

General Techniques.- Proteomics and the Molecular Biologist.- Protein Identification from 2-D Gels Using In Vitro Transcription Translation Products.- Selective Chemical Cleavage Methods in Proteomics, Including C-Terminal Successive Degradation.- Means of Hydrolyzing Proteins Isolated upon ProteinChip® Array Surfaces.- A Combined Radiolabeling and Silver Staining Technique for Improved Visualization and Localization of Proteins on Two-Dimensional Gels.- Qualitative and Quantitative Proteomic Analyses via Multidimensional Protein Identification Technology.- Di- and Tri-Chromatic Fluorescence Detection on Western Blots.- Multiplexed Proteomics.- A Strategy for Characterizing Antibody/Antigen Interactions Using ProteinChip® Arrays.- Stable Isotope Labeling with Amino Acids as an Aid to Protein Identification in Peptide Mass Fingerprinting.- The Use of 18O Labeling as a Tool for Proteomic Applications.- Automated Nanoflow Liquid Chromatography/Tandem Mass Spectrometric Identification of Liver Mihondrial Proteins.- In Silico Proteomics.- Post-Translational Modifications, Variants, and Isoforms.- Predicting Glycan Composition from Experimental Mass Using GlycoMod.- Querying GlycoSuiteDB.- New Tools for Quantitative Phosphoproteome Analysis.- Computer-Aided Strategies for Characterizing Protein Isoforms.- Protein Variant Separations Using Cation Exchange Chromatography on Grafted, Polymeric Stationary Phases.- Specific Systems.- Noninvasive Imaging of Protein-Protein Interactions in Living Animals.- Strategies in Clinical Proteomics.- Proteomic Profiling of the Cancer Microenvironment.- Identification of Determinants of Sensitivity to Antitumor Drugs.- Application of Proteomics to the Discovery of Serological Tumor Markers.- Infectomic Analysis of Microbial InfectionsUsing Proteomics.- Toward a Complete Proteome of Bacillus subtilis.- Renal and Urinary Proteomics.- Proteomics in Endocrinology.- Proteomics in Plant Biology.- Data Analysis.- Bioinformatics in Proteomics.- Quantitative Characterization of Proteomics Maps by Matrix Invariants.- Complexity of Protein-Protein Interaction Networks, Complexes, and Pathways.- Patchwork Peptide Sequencing.- Estimation of Bias in Proteome Research.- Scoring Functions for Mass Spectrometric Protein Identification.

Editorial Reviews

"...covers recent advances and also acts as a manual of well-practiced techniques. This volume should appeal particularly to the uninitiated but contains several articles of interest to more experienced practitioners...As well as being an excellent guide, this volume in its entirety makes a strong case for the advantages in the application of these post-genomic technologies" - Rapid Communications in Mass Spectrometry

"Handbook of Proteomic Methods includes a wide range of techniques for identifying and analyzing proteins expressed in cells. As many other Humana Press method books, this is another must-have for large academic libraries supporting research in complex biological systems and drug development." -E-STREAMS

"This is a very well produced volume with excellent diagrams and reproduction of difficult images such as 2-D gels...deserves a place on the proteomic researchers' bookshelf." - Chromatographia

From the Publisher