Herpes Simplex Virus Protocols / Edition 1

by S. Moira Brown, Alasdair R. MacLean

Overview

Herpes Simplex Virus Prools comprises a wide range of experimental prools that should be especially useful to new workers in herpes virology. Hopefully, it will also provide information for those with experience in the field, as well as those embarking on techniques that are new to them. Obviously the range of topics covered cannot be comprehensive, but we have tried to provide prools dealing with those procedures that are most widely used; and we have selected expert authors accordingly. We have also tried to cover the range from the more biological in vivo maneuvers to purely molecular procedures, taking into account the topical interest in the potential use of HSV as a therapeutic tool. In this way there should be sufficient inf- mation for most procedures the average herpes virologist is likely to require— at least at this moment in time! Since the herpesviruses are a large family, we have largely based the prools on the virus we know best—herpes simplex virus. With this as the prototype, it should be relatively easy to extrapolate and make the necessary modifications required for application to some of the other herpesviruses, especially members of the alpha group, such as PRV and EHV. It would have been an impossible task to include chapters covering the unique aspects of each known herpesvirus.

Product Details

ISBN-13:	9781617370137
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/09/2010
Series:	Methods in Molecular Medicine , #10
Edition description:	Softcover reprint of hardcover 1st ed. 1998
Pages:	419
Product dimensions:	5.98(w) x 9.02(h) x 0.35(d)

About the Author

Brown, S. Moira (Univ of Glasgow); MacLean, Alasdair R. (Univ of Glasgow)

The contributors represent the specialties of virology, microbiology, immunology, pathology, molecular genetics, neurology, human genetics, and ophthalmology. Most come from universities and research institutes in the U.S., the U.K., the Netherlands, Israel, and Norway. Institutions prominently represented include Univ of Glasgow, Univ of Pennsylvania, Louisiana State Univ, and Netherlands Cancer Institute.

Table of Contents

HSV Growth, Preparation, and Assay.- HSV Entry and Spread.- Preparation of HSV-DNA and Production of Infectious Virus.- HSV Mutagenesis.- Saturating Mutagenesis and Characterization of a Herpesvirus Genome Using In Vivo Reconstitution of Virus from Cloned Subgenomic Regions.- Construction and Use of Cell Lines Expressing HSV Genes.- Analysis of HSV Polypeptides.- Protein Purification.- Expression and Purification of Secreted Forms of HSV Glycoproteins from Baculovirus-Infected Insect Cells.- Crystallization of Macromolecules for Three-Dimensional Structure Determination.- Direct Immunogold Labeling of Herpesvirus Suspensions.- Expression of HSV Proteins in Bacteria.- In Vitro Systems to Analyze HSV Transcript Processing.- Analysis of HSV-1 Transcripts by RNA-PCR.- Transient Assays for HSV Origin and Replication Protein Function.- HSV Amplicons in Gene Therapy.- Analyses of HSV Proteins for Posttranslational Modifications and Enzyme Functions.- HSV-Cellular Protein Interactions.- Models of Recurrent Infection with HSV in the Skin and Eye of the Mouse.- Pathogenesis and Molecular Biology of HSV Latency and Ocular Reactivation in the Rabbit.- HSV Latency In Vitro.- Assessing Cell-Mediated Immune Responses to HSV in Murine Systems.- Assays for HSV Gene Expression During Establishment and Maintenance of Latent Infection.- Analysis of HSV-DNA and RNA Using the Polymerase Chain Reaction.- HSV Vectors for Gene Therapy.- Investigation of the Anti-HSV Activity of Candidate Antiviral Agents.

Editorial Reviews

3 Stars from Doody

This is a collection of complete procedures for basic methods used in research on herpes simplex virus. The book is designed to provide protocols to cover a wide range of topics. The procedures range from the very basic, such as propagation of the virus, to more complex protocols for genetic methods, studies on regulation of virus replication in cell culture systems, and animal models. It is intended as a reference book. Students just beginning their studies on herpes viruses will find this resource useful, as will more experienced scientists who are beginning studies with the herpes simplex virus. Herpes virologists who are initiating studies in a new aspect of the field will also find these detailed descriptions of techniques helpful. The editors have compiled individual chapters written by authorities with extensive experience using the techniques they describe. This results in detailed, complete descriptions of materials and methods. Extremely useful information on rationales, expected results, problems that may arise, and evaluation of results are also presented. Illustrations are limited, but those presented are helpful. References to historically important studies in which the methods were developed are included as well as more recent references illustrating how these protocols have been used to answer questions about the herpes simplex virus. The wisdom derived from the extensive experience of the collected contributors will be especially useful for neophyte researchers.

Jerry Lynn Taylor

Reviewer: Jerry Lynn Taylor, PhD (Medical College of Wisconsin)
Description: This is a collection of complete procedures for basic methods used in research on herpes simplex virus.
Purpose: The book is designed to provide protocols to cover a wide range of topics. The procedures range from the very basic, such as propagation of the virus, to more complex protocols for genetic methods, studies on regulation of virus replication in cell culture systems, and animal models. It is intended as a reference book.
Audience: Students just beginning their studies on herpes viruses will find this resource useful, as will more experienced scientists who are beginning studies with the herpes simplex virus. Herpes virologists who are initiating studies in a new aspect of the field will also find these detailed descriptions of techniques helpful.
Features: The editors have compiled individual chapters written by authorities with extensive experience using the techniques they describe. This results in detailed, complete descriptions of materials and methods. Extremely useful information on rationales, expected results, problems that may arise, and evaluation of results are also presented. Illustrations are limited, but those presented are helpful. References to historically important studies in which the methods were developed are included as well as more recent references illustrating how these protocols have been used to answer questions about the herpes simplex virus.
Assessment: The wisdom derived from the extensive experience of the collected contributors will be especially useful for neophyte researchers.

Doody's Review Service