Herpes Simplex Virus: Methods and Protocols

by Russell J. Diefenbach, Cornel Fraefel

Overview

Herpes Simplex Virus: Methods and Protocols provides a wide collection of protocols employed in various levels of herpes virus research, including basic protocols on growing viruses in cell culture and cloning, manipulating and preparing viral DNA. Other chapters describe approaches to design and apply HSV-1 vectors for vaccination, cancer and gene therapy or to study specific aspects of HSV-1 biology such as latency, intracellular transport and protein-protein interaction. Procedures for structural analyses, microscopy, proteomics and testing of antivirals are included as well. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Herpes Simplex Virus: Methods and Protocols will aid new researchers in the field of herpes virology as well as those experienced investigators wishing to embark on new techniques.

Product Details

ISBN-13:	9781493954070
Publisher:	Springer New York
Publication date:	08/23/2016
Series:	Methods in Molecular Biology , #1144
Edition description:	Softcover reprint of the original 1st ed. 2014
Pages:	331
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

HSV-1 Biology and Life Cycle.- Herpes Simplex Virus Growth, Preparation, and Assay.- Isolation of Herpes Simplex Virus Nucleocapsid DNA.- Construction and Characterization of Bacterial Artificial Chromosomes (BACs) Containing Herpes Simplex Virus Full-Length Genomes.- Engineering HSV-1 Vectors for Gene Therapy.- Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors.- HSV-1 Amplicon Vectors as Genetic Vaccines.- Modification of HSV-1 to an Oncolytic Virus.- Herpes Simplex Virus Mutant Generation and Dual Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo.- Phenotypic and Genotypic Testing of HSV-1 Resistance to Antivirals.- Using Homogeneous Primary Neuron Cultures to Study Fundamental Aspects of HSV-1 Latency and Reactivation.- Characterization of Extracellular HSV-1 Virions by Proteomics.- A Precipitation-Based Assay to Analyze Interactions of Viral Particles with Cytosolic Host Factors.- Affinity Purification Combined with Mass Spectrometry to Identify Herpes Simplex Virus Protein-Protein Interactions.- Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy.- Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication.- Expression, Purification and Crystallization of HSV-1 Glycoproteins for Structure Determination.- Cryo-EM Techniques to Resolve the Structure of HSV-1 Capsid-Associated Components.- In Vivo HSV-1 DNA Transport Studies Using Murine Retinal Ganglion Cells.- HSV-1 Protein Expression Using Recombinant Baculoviruses.- The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines.

Editorial Reviews

Reviewer: Omer Iqbal, MD, FACC, FESC (Loyola University Medical Center)
Description: This book, in its second edition, expands on the previous edition by providing updated methods used to study the herpes simplex virus (HSV) and including next generation sequencing, CRISPR/Cas9 engineering, and the use of BIOID to identify protein interactions. The first edition was published in 2014.
Purpose: The purpose is to help researchers in the herpes virus field in their quest for understanding the complex interaction between herpes virus and host. These are extremely worthy objectives. The book has met these objectives by providing cutting-edge and comprehensive information on HSV methods and protocols.
Audience: The book is intended to help researchers in the field of herpes simplex virus research in understanding complex interactions between herpes virus and host. In my opinion, in addition to the researchers, the book will be quite useful for students and virologists who wish to further their knowledge in this field. The book does meet the needs of the intended audience by providing comprehensive, cutting-edge information on HSV methods and protocols. The authors are credible authorities by virtue of their qualifications, experience, and number of publications in this field.
Features: The book is divided into 27 chapters, each written by experts in their field. The earlier chapters deal with the HSV-1 biology and life cycle and the current information in antiviral and vaccine development. Other chapters cover approaches to the design and application of HSV-1 vectors for cancer and gene therapy; CRISPR/Cas9-based genome editing of HSV; and HSV mutant generation and dual detection methods. Other very interesting chapters deal with transmission immunoelectron microscopy of HSV and multiflourescence live analysis of HSV type-1 replication. The concluding chapter provides an interesting account on the Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA vaccines. The book includes several high-quality illustrations that make the subject matter easy to understand. A detailed index may have facilitated easy location of different topics.
Assessment: This book provides comprehensive and cutting-edge information on the methods and protocols related to HSV research. The book is quite unique with different chapters written by experts in the field. Although other books in the field provide extensive coverage on HSV, this second edition gives interesting, updated information that fully justifies replacing the previous edition.

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