High-Throughput Next Generation Sequencing: Methods and Applications

by Young Min Kwon (Editor), Steven C. Ricke (Editor)

Overview

Due to their novel concepts and extraordinary high-throughput sequencing capacity, the “next generation sequencing” methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities. These methods are now being recognized as essential tools for a more comprehensive and deeper understanding of the mechanisms underlying many biological processes. In High-Throughput Next Generation Sequencing: Methods and Applications, experts in the field explore the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities; however, the methods described in this book will also offer general applications relevant to the study of any living organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, High-Throughput Next Generation Sequencing: Methods and Applications is an excellent collection of chapters to aid all scientists who wish to apply these innovative research tools to enhance their own pursuits in microbiology and also biology in general.

Product Details

ISBN-13:	9781493961641
Publisher:	Springer-Verlag New York, LLC
Publication date:	08/23/2016
Series:	Methods in Molecular Biology , #733
Edition description:	Softcover reprint of the original 1st ed. 2011
Pages:	308
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Helicos Single Molecule Sequencing of Bacterial Genomes.- Whole Genome Sequencing of Unculturable Bacterium Using Whole Genome Amplification.- RNA Sequencing and Quantitation Using the Helicos Genetic Analysis System.- Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing.- Discovery of Bacterial sRNAs by High-Throughput Sequencing.- Identification of Virus Encoding MicroRNAs Using 454 FLX Sequencing Platform.- Ribosomal RNA Depletion for Massively Parallel Bacterial RNA-Sequencing Applications.- Integrating High-Throughput Pyrosequencing and Quantitative Real-Time PCR to Analyze Complex Microbial Communities.- Tag-Encoded FLX Amplicon Pyrosequencing for the Elucidation of Microbial and Functional Gene Diversity in Any Environment.- Pyrosequencing of Chaperonin-60 (cpn60) Amplicons as a Means of Determining Microbial Community Composition.- Prescreening of Microbial Populations for the Assessment of Sequencing Potential.- Metagenomics.- Metagenomic Analysis of Intestinal Microbiomes in Chickens.- Gene Expression Profiling: Metatranscriptomics.- High-Throughput Insertion Tracking by Deep Sequencing for the Analysis of Bacterial Pathogens.- Determining DNA Methylation Profiles Using Sequencing.- Preparation of Next-Generation Sequencing Libraries Using Nextera™ Technology: Simultaneous DNA Fragmentation and Adaptor Tagging by In Vitro Transposition.- Amplification-Free Library Preparation for Paired-End Illumina Sequencing.- Target-Enrichment through Amplification of Hairpin-Ligated Universal Targets for Next-Generation Sequencing Analysis.- 96-Plex Molecular Barcoding for the Illumina Genome Analyzer.

Editorial Reviews

From the reviews:

“This well-illustrated book details the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities, in addition to general applications relevant to the study of any living organism. … The book is intended for scientists interested in this area, but even graduate students and other researchers field may find this book useful.” (Omer Iqbal, Doody’s Book Reviews, March, 2012)

“There are definitely some highlights that will make this book of use to both NGS Newbies and experienced practitioners. … many readers will find this a very useful book.” (Michael A. Quail, Human Genetics, Vol. 130, 2011)

From the Publisher