Human Cytomegaloviruses: Methods and Protocols

by Andrew D. Yurochko (Editor)

Overview

This new edition explores and provides an update on the biology and pathogenesis of human cytomegalovirus infection. Modern techniques that are currently being utilized to investigate the molecular aspects of viral infection, as well as how these new research studies are leading to new approaches to mitigate disease, are also provided. Given the key role the virus plays in significant acute and chronic human disease in all stages of life, from newborns to seniors, the need for clear methodologies to further explore the biology of HCMV infection and mitigation strategies is readily apparent. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Human Cytomegaloviruses: Methods and Protocols, Second Edition serves as an ideal scientific reference for basic and clinical scientists and medical personnel on the modern understanding of the pathobiology of the virus, and the approaches, techniques, and models to study human cytomegalovirus infection and disease.

Product Details

ISBN-13:	9781071611135
Publisher:	Springer US
Publication date:	02/09/2022
Series:	Methods in Molecular Biology , #2244
Edition description:	2nd ed. 2021
Pages:	468
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

History of the Molecular Biology of Cytomegalovirus.- Overview of Human Cytomegalovirus Pathogenesis.- Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies.- Use of Diploid Human Fibroblasts as a Model System to Culture/Grow, Study and Understand Human Cytomegalovirus Infection.- Use of Recombinant Approaches to Construct Human Cytomegalovirus Mutants.- The Use of Primary Human Cells (Fibroblasts, Monocytes and Others) to Assess Human Cytomegalovirus Function.- Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation.- Analysis of Cytomegalovirus Binding/Entry-Mediated Events.- Use of 5-Ethynyl-2’-Deoxyuridine Labelling and Flow Cytometry to Study Cell Cycle-Dependent Regulation of Human Cytomegalovirus Gene Expression.- Methods for Studying the Function of Cytomegalovirus GPCRs.- Methods for the Detection of Cytomegalovirus in Glioblastoma Cells and Tissues.- Methods to Study the Nucleocytoplasmic Transport of Macromolecules with Respect to Their Impact on the Regulation of Human Cytomegalovirus Gene Expression.- Fluorescence-Based Laser Capture Microscopy Technology Facilitates Identification of Critical In Vivo Cytomegalovirus Transcriptional Programs.- Techniques for Characterizing Cytomegalovirus-Encoded miRNAs.- What We Have Learned From Animal Models of HCMV.- Rodent Models of Congenital Cytomegalovirus Infection.- Recent Approaches and Strategies in the Generation of Anti-HumanCytomegalovirus Vaccines.- Approaches for the Generation of New Anti-Cytomegalovirus Agents:  Identification of Protein-Protein Interaction Inhibitors and Compounds Against the HCMV IE2 Protein.