Tens of thousands of hydrocarbons, their derivatives and lipids play vital roles in all organisms, from structural cellular components to signalling molecules. The global hydrocarbon cycle, largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be vital in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges.
Springer Protocols provide a variety of readily reproducible laboratory and field methods essential to research in hydrocarbon and lipid microbiology, covering topics as diverse as ecology and synthetic biology. Each chapter first explains the principles underlying the methods presented, followed by step-by-step protocols. Helpful notes provide details on the essential tips and tricks that will promote good results, and so advance the field.
Table of Contents Introduction to Cultivation.- Cultivating fastidious microbes.- The essentials of cultivating aerobic hydrocarbon-degrading microbes.- Cultivating aerobic hydrocarbon-degrading microbes from microalgae.- Growing and maintaining hydrocarbon-degrading consortia.- Two-phase cultivation techniques for hydrocarbon-degrading organisms.- Cultivation of hydrocarbon-degrading fungi.- Growth of hydrocarbon-degrading bacteria in continuous culture.- Growth procedures at low substrate concentrations.- Cultivation of biofilm-forming hydrocarbon degraders.- Cultivation of asphaltene-degrading microbes.- Cultivation of microorganisms for fuel quality improvement.- Simulation of oxygen and redox oscillations in crude oil- degrading bioreactors.- Cultivation of anaerobic microorganisms with hydrocarbons as growth substrates.- Enrichment and isolation of metal respirers and hydrocarbon oxidizers.- Enrichment and isolation of oxyion-respiring hydrocarbon oxidizers.- Cultivation of methanogens.- Cultivation of methylotrophs.- Cultivation of methanotrophs.- Cultivation of extremophilic hydrocarbon degraders.- Cultivation of piezophilic hydrocarbon degraders.- Cultivation of oil-producing microalgae.- Cultivation of oleaginous yeasts.- Cultivation of oil-tolerant protists.