ISBN-10:
1617370789
ISBN-13:
9781617370786
Pub. Date:
11/10/2010
Publisher:
Springer-Verlag New York, LLC
Immunocytochemical Methods and Protocols / Edition 2

Immunocytochemical Methods and Protocols / Edition 2

by Lorette C. Javois
Current price is , Original price is $129.0. You

Temporarily Out of Stock Online

Please check back later for updated availability.

Product Details

ISBN-13: 9781617370786
Publisher: Springer-Verlag New York, LLC
Publication date: 11/10/2010
Series: Methods in Molecular Biology , #115
Edition description: Softcover reprint of 2nd ed. 1999
Pages: 465
Product dimensions: 5.98(w) x 9.02(h) x 0.35(d)

Table of Contents

Part I. Antibody Preparation. Overview of Antibody Use in Immunocyhemistry, Su-Yau Mao, Lorette C. Javois, and Ute M. Kent. Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration, Ute M. Kent. Purification of Antibodies Using Ion-Exchange Chromatography, Ute M. Kent. Purification of Antibodies Using Affinity Chromatography, Ute M. Kent. Purification of Antibodies Using Protein A-Sepharose and FPLC, Ute M. Kent. Conjugation of Fluorochromes to Antibodies, Su-Yau Mao. Biotinylation of Antibodies, Su-Yau Mao. Part II. Tissue Preparation for light Microscopic Analyses. Overview of Cell Fixatives and Cell Membrane Permeants, Melissa A. Melan. Preparation of Frozen Sections for Analysis, Gary L. Bratthauer.
Processing of Cytological Specimens, Gary L. Bratthauer. Processing of Tissue Culture Cells, Gary L. Bratthauer. Processing of Tissue Specimens, Gary L. Bratthauer. Antigen Retrieval for Immunohishemical Reactions in Routinely Processed Paraffin Sections, Laszlo Krenacs, Tibor Krenacs, and Mark Raffeld. Part III. Light Microscopic Detection Systems. Overview of Fluorochromes, J. Michael Mullins. Direct Immunofluorescent Labeling of Cells, Lorette C. Javois. Fluorescence Labeling of Surface Antigens on Attached or Suspended Tissue-Culture Cells, Mark C. Willingham. Fluorescence Labeling of Intracellular Antigens in Attached or Suspended Tissue-Culture Cells, Mark C. Willingham. Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts, Lorette C. Javois. TUNEL Assay for Apoptotic Cells, Virginia M. Heatwole. Overview of Fluorescence Analysis with the Confocal Microscope, Liana Harvath. Overview of Laser Microbeam Applications as Related to Antibody Targeting, P. Scott Pine. Overview of Fluorescence Photomicography, Lorette C. Javois and J. Michael Mullins. Overview of Antigen Detection Through Enzymatic Activity, Gary L. Bratthauer. The Peroxidase Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods, Gary L. Bratthauer. The Avidin Biotin Complex (ABC) Method and Other Avidin Biotin Binding Methods,
Gary L. Bratthauer. Avidin Biotin Labeling of Cellular Antigens in Cryostat Sectioned Tissue, Mark Raffeld and Elaine S. Jaffe.
Multiple Antigen Immunostaining Procedures, Laszlo Krenacs, Tibor Krenacs, and Mark Raffeld. ELISA on Attachment Dependent or Suspension Grown Cells, Julie Ann Brent.. Use of Immunogold with Silver Enhancement ,Constance Oliver. Part IV. Flourescence Activated Cell Sorter (FACS) Analyses. Overview of Flow Cytometry and Fluorescent Probes for Cytometry,
Robert E. Cunningham. Tissue Disaggregation, Robert E. Cunningham. Indirect Immunofluorescent Labeling of Viable Cells,
Robert E. Cunningham. Indirect Immunofluorescent Labeling of Fixed Cells, Robert E. Cunningham. Fluorescent Labeling of DNA,
Robert E. Cunningham. Deparaffinization and Processing of Pathologic Material, Robert E. Cunningham. Assay for Phagocytosis,
Liana Harvath and Douglas A. Terle. Assay for Filamentous Actin, Liana Harvath. Assay for Chemoattractant Binding, Liana Harvath, Robert R. Aksamit, and Robert E. Cunningham. Assay for Oxidative Metabolism, Liana Harvath and Douglas A. Terle.
Part V. Colloidal Gold Detection Systems For Electron Microscopic Analysis. Fixation and Embedding, Constance Oliver. Preparation of Colloidal Gold, Constance Oliver. Conjugation of Colloidal Gold to Proteins, Constance Oliver. Colloidal Gold/Streptavidin Methods, Constance Oliver. Pre-Embedding Labeling Methods, Constance Oliver. Postembedding Labeling Methods, Constance Oliver. Part VI.Applications For Nucleic Acid Localization By In Situ Hybridization. Overview of In Situ Hybridization, Roland M. Nardone. In Situ Hybridization to Human Chromosomes of an Alkaline Phosphatase Labeled Centromeric Probe, Roland M. Nardone. Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes,
Roland M. Nardone. In Situ RT-PCR and Hybridization Techniques, Vishakha Thaker. Part VII. Special Applications For the Clinical Laboratory. Overview of the Clinical Immunohishemistry Laboratory: Regulations and Troubleshooting Guidelines,
Patricia A. Fetsch and Andrea Abati. Overview of Immunohishemical Approaches to the Differential Diagnosis of Tumors,
Dennis M. Frisman. Overview of Automated Immunostainers, Gilbert E. Herman, Edna A. Elfont, and Alton D. Floyd.

Customer Reviews

Most Helpful Customer Reviews

See All Customer Reviews