Table of Contents
Preface…
Table of Contents…
Contributing Authors…
1. Introduction to the Immune System
Scott McComb, Aude Thiriot, Bassel Akache, Lakshmi Krishnan, and Felicity Stark
2. Immunoproteomics Methods and Techniques
Kelly M. Fulton, Isabel Baltat, and Susan M. Twine
3. Classical Immunoproteomics: Serological Proteome Analysis (SERPA) for Antigen Identification
Kelly M. Fulton, Anna Ananchenko, Lawrence Wolfraim, Shannon Martin, and Susan M. Twine
4. Profiling of Cytokine and Chemokine Responses using Multiplex Bead Array Technology
Greg Harris and Wangxue Chen
5. Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis
Timothy D. Veenstra
6. Enriching for Low Abundance Serum Proteins using ProteoMiner™ and Protein Level HPLC
Sophie Moggridge, Kelly M. Fulton, and Susan M. Twine
7. Identification of the Antigen Content of Electroimmunoprecipitates
N. Helena Beyer and Niels H. H. Heegaard
8. Detection of Human Norovirus-Specific Antibodies using the Luciferase Immunoprecipitation System (LIPS) Assay
Christine M. Tin and Stanislav V. Sosnovtsev
9. Intact Mass Spectrometry Analysis of Immuno-Isolated Human Therapeutic Antibodies from Serum
Christie E. Delaney, John F. Kelly, Wen Ding, and Arsalan S. Haqqani
10. Antigen Identification for Cell-Binding Antibodies using Ligand Directed Crosslinking and Biotin Transfer
Tammy-Lynn Tremblay and Jennifer J. Hill
11. Whole Genome-Phage Display Libraries: A Powerful Tool for Antigen Discovery
Elisa Beghetto and Nicola Gargano
12. Multiplexed Detection of Autoantibodies to Glycopeptides using Microarray
Johannes W. Pedersen, Alexander Nøstdal, and Hans H. Wandall
13. Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification
Fei Wen, Mason R. Smith, and Huimin Zhao
14. Isolation of Major Hisompatibility Complex (MHC)-Associated Peptides by Immunoaffinity Purification
Sarah A. Penny and Stacy A. Malaker
15. Enhanced Mass Spectrometry Detection of MHC Peptides
Rui Chen and Jianjun Li
16. Enrichment of Phosphorylated MHC Peptides with Immobilized Metal Affinity Chromatography and Titanium Dioxide Particles
Rui Chen and Jianjun Li
17. Mass Spectrometric Identification and Molecular Modeling of Glycopeptides Presented by MHC Class I and II Processing Pathways
Stacy A. Malaker and Michael J. Ferracane
18. Genome Based Bioinformatic Prediction of Major Hisompatibility (MHC)
Simon J. Foote
19. Enhancing Mass Spectrometry-Based MHC-I Peptide Identification through a Targeted Database Search Approach
Prathyusha Konda, J. Patrick Murphy, Morten Nielsen, and Shashi Gujar
20. Prioritization of Therapeutic Targets of Inflammation Using Proteomics, Bioinformatics, and In Silico Cell-Cell Interactomics
Arsalan S. Haqqani and Danica B. Stanimirovic
21. Genomics Driven Immunoproteomics: An Integrative Platform to Uncover Important Biomarkers for Human Diseases
Raghavendra Giri, Veneta Qendro, Pooja Rani, Carren Jepchumba, Grace Bugos, Volker Stadler, and David K. Han
22. Identification of Antigens in Immune Complexes
Nozomi Aibara and Kaname Ohyama
23. Analysis and Characterization of Immune Cells and their Activation Status by Whole-Cell MALDI TOF Mass Spectrometry
Richard Ouedraogo, Julien Textoris, Laurent Gorvel, Aurélie Daumas, Christian Capo, and Jean-Louis Mege
24. A Molecular Immunoproteomics Approach to Assess the Viral Antigenicity of Influenza
Kevin M. Downard
25. Immunoproteomic Biomarkers: From Publication to Personalized Medicine
Devanand Pinto
