Insertional Mutagenesis Strategies in Cancer Genetics / Edition 1

by Adam J. Dupuy, David A. Largaespada

Overview

The goal of this work is summarize the contribution that insertional mutagenesis has made to our understanding of cancer. A variety of insertional mutagens are presented that have been used to study a variety of tumor types in several model organisms. In addition, the impact of insertional mutagenesis in several gene therapy trials is discussed along with strategies to avoid such complications in future clinical trials.

Product Details

ISBN-13:	9781441976550
Publisher:	Springer New York
Publication date:	11/19/2010
Edition description:	2011
Pages:	200
Product dimensions:	6.30(w) x 9.30(h) x 1.00(d)

About the Author

Adam Dupuy, Ph.D., is an Assistant Professor at the Carver College of Medicine at the University of Iowa, and a rising star in the field of insertional mutagenesis; two of the last of his publications have been in Nature, and he was the lead author on one of these. Dr. Dupuy earned his B.A. at Central College in Pella, Iowar, went on to earn his Ph.D. at the University of Minnesota in the Program in Molecular, Cellular, Developmental Biology & Genetics, and did his Postdoctoral fellowship in the Mouse Cancer Genetics Program at the NCI-Frederick.

Table of Contents

1 Insertional Mutagenesis: A Powerful Tool in Cancer Research Anton Berns 1

2 Retroviral Insertional Mutagenesis in Mouse Models of Leukemia and Lymphoma David A. Largaespada 19

3 Gene Discovery by MMTV Mediated Insertional Mutagenesis Annabel Vendel-Zwaagstra John Hilkens 39

4 Chicken Models of Retroviral Insertional Mutagenesis Vladimír Pecenka Petr Pajer Vít Karafiát Michal Dvorák 77

5 Sleeping Beauty Models of Cancer Jesse D. Riordan Laura M. Rogers Katherine E. Berquam-Vrieze Adam J. Dupuy 113

6 Insertional Mutagenesis in Hematopoietic Cells: Lessons Learned from Adverse Events in Clinical Gene Therapy Trials Lars U. Müller Michael D. Milsom David A. Williams 131

7 Bioinformatics of High-Throughput Insertional Mutagenesis Keiko Akagi Ming Yi Jean Roayaei Robert M. Stephens 167

Index 189