Laboratory Experiments in Microbiology

by Ted Johnson, Christine Case

Overview

For general microbiology laboratory courses

Laboratory Experiments in Microbiology features 57 thoroughly class-tested and easily customizable exercises that teach basic microbiology techniques and applications. The manual provides comprehensive coverage of every area of microbiology across diverse disciplines, including the biological sciences, allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The lab manual is the perfect companion to Tortora/Funke/Case’s Microbiology: An Introduction, 13th Edition or any introductory microbiology text.

The 12th Edition of Laboratory Experiments in Microbiology is easier than ever to navigate and more visually effective with new icons indicating when an exercise addresses the human or environmental microbiome, is investigative, or addresses an ASM guideline. New ASM Thinking Skills outline the steps that help develop laboratory thinking skills. Pre-lab quizzes in Mastering^TM Microbiology ensure students arrive prepared for each lab, and activities such as Lab and Lecture: Putting It All Together help students see how lab and lecture are integrated.

Product Details

ISBN-13:	9780134605203
Publisher:	Pearson Education
Publication date:	01/01/2018
Series:	What's New in Microbiology Series
Edition description:	Lab Manual
Pages:	512
Product dimensions:	8.90(w) x 10.60(h) x 0.70(d)

About the Author

About our authors

Ted R. Johnson is a professor emeritus of biology at St. Olaf College, a liberal arts college in Northfield, Minnesota, where he has taught courses in microbiology and immunology. He has taught at St. Olaf for 39 years and previously taught at Mankato State University for five years. He received his master’s degree and his Ph.D in microbiology from the University of Illinois in Chicago, Illinois. While at St. Olaf College, he has developed and directed several semester abroad and interim programs. He has been involved in advising health professions students and received the Fr.Joseph Walter Award for Health Profession advising. His research focuses on the immune response to virally induced cancer in various animal models.

Christine L. Case is a professor of microbiology at Skyline College in San Bruno, California, where she has taught for the past 47 years. She received her Ed. D. in curriculum and instruction from Nova Southeastern University and her M.A. in microbiology from San Francisco State University. She was Director for the Society for Industrial Microbiology (SIM). She received the ASM and California Hayward outstanding educator awards and SACNAS Mentor award. In addition to teaching, Chris contributes regularly to professional literature, develops innovative educational methodologies, and maintains a personal and professional commitment to conservation and the importance of science in society. Chris is also an avid photographer, and many of her photographs appear in this lab manual.

Table of Contents

PART ONE MICROSCOPY

1. Use and Care of the Microscope
2. Examination of Living Microorganisms

PART TWO HANDLING BACTERIA

1. Microbes in the Environment
2. Transfer of Bacteria: Aseptic Technique

PART THREE STAINING METHODS

1. Preparation of Smears and Simple Staining
2. Negative Staining
3. Gram Staining
4. Acid-Fast Staining
5. Structural Stains (Endospore, Capsule, and Flagella)
6. Morphological Unknown

PART FOUR CULTIVATION OF BACTERIA

1. Isolation of Bacteria by Dilution Techniques
2. Special Media for Isolating Bacteria

PART FIVE MICROBIAL METABOLISM

1. Carbohydrate Catabolism
2. Fermentation
3. Protein Catabolism, Part 1
4. Protein Catabolism, Part 2
5. Respiration
6. Unknown Identification and Bergey’s Manual

PART SIX MICROBIAL GROWTH

1. Oxygen and the Growth of Bacteria
2. Determination of a Bacterial Growth Curve: The Role of Temperature
3. Biofilms

PART SEVEN CONTROL OF MICROBIAL GROWTH

1. Physical Methods of Control: Heat
2. Physical Methods of Control: Ultraviolet Radiation
3. Chemical Methods of Control: Disinfectants and Antiseptics
4. Chemical Methods of Control: Antimicrobial Drugs
5. Effectiveness of Hand Scrubbing

PART EIGHT MICROBIAL GENETICS

1. Regulation of Gene Expression
2. Isolation of Bacterial Mutants
3. Transformation of Bacteria
4. DNA Fingerprinting
5. Genetic Engineering
6. Ames Test for Detecting Possible Chemical Carcinogens

PART NINE THE MICROBIAL WORLD

1. Fungi: Yeasts and Molds
2. Phototrophs: Algae and Cyanobacteria
3. Protozoa
4. Parasitic Helminths

PART TEN VIRUSES

1. Isolation and Titration of Bacteriophages
2. Plant Viruses

PART ELEVEN INTERACTION OF MICROBE AND HOST

1. Epidemiology
2. Koch's Postulate

PART TWELVE IMMUNOLOGY

1. Innate Immunity
2. Agglutination Reactions: Slide Agglutination
3. Agglutination Reactions: Microtiter Agglutination
4. ELISA Technique

PART THIRTEEN MICRORGANISMS AND DISEASE

1. Bacteria of the Skin
2. Bacteria of the Respiratory Tract
3. Bacteria of the Mouth
4. Bacteria of the Gastrointestinal Tract
5. Bacteria of the Urogenital Trac
6. Identification of an Unknown from a Clinical Sample
7. Rapid Identification Methods

PART FOURTEEN MICROBIOLOGY AND THE ENVIRONMENT

1. Microbes in Water: Multiple-Tube Technique
2. Microbes in Water: Membrane Filter Technique
3. Microbes in Food: Contamination
4. Microbes Used in the Production of Foods
5. Microbes in Soil: The Nitrogen and Sulfur Cycles
6. Microbes in Soil: Bioremediation

APPENDICES

• A. Pipetting
• B. Dilution Techniques and Calculations
• C. Use of the Spectrophotometer
• D. Graphing
• E. Use of the Dissecting Microscope
• F. Use of the Membrane Filter
• G. Electrophoresis
• H. Keys to Bacteria

Editorial Reviews

A laboratory companion to , Sixth Edition by Tortora, Funke, and Case. The manual contains 56 class- tested exercises, including all those recommended by the Committee on Undergraduate and Graduate Education of the American Society for Microbiology. Sections cover microscopy, staining methods, cultivation of bacteria, microbial metabolism, microbial growth, control of microbial growth, microbial genetics, the microbial world, viruses, interaction of microbe and host, immunology, microorganisms and disease, and microbiology and the environment. A color photo quiz requires students to identify an unknown. Spiral wire binding. Annotation c. by Book News, Inc., Portland, Or.

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