Laser Capture Microdissection: Methods and Protocols / Edition 3

by Graeme I. Murray

Overview

Pathologists and other medical researchers offer practical advice on how to carry out tissue-based laser microdissection in laboratories using the microdissection systems available, and how to apply a wide range of molecular technologies. They cover DNA analysis, RNA and gene expression studies using microdissected cells, applications in proteomics, and the molecular analysis of microorganisms. Among specific topics are detecting genetic aberrations in early and advanced human urinary bladder cancer, analyzing macrophage gene expression from atherosclerotic lesions, colorectal cancer proteomics, and polymerase chain reaction for analyzing human papilla virus infection. Annotation ©2004 Book News, Inc., Portland, OR

Product Details

ISBN-13:	9781493975570
Publisher:	Springer New York
Publication date:	01/17/2018
Series:	Methods in Molecular Biology , #1723
Edition description:	3rd ed. 2018
Pages:	412
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Laser Capture Microdissection: Methods and Applications.- Laser Microdissection for Gene Expression Profiling.- Gene Expression Using the PALM System.- Immuno-Guided Microdissection Techniques.- Optimized RNA Extraction from Non-Deparaffinized, Laser-Microdissected Material.- Laser Capture Microdissection for Analysis of Gene Expression in Formalin-Fixed Paraffin-Embedded Tissue.- MicroRNA Profiling Using RNA from Microdissected Immunostained Tissue.- Profiling Solid Tumor Heterogeneity by LCM and Biological MS of Fresh Frozen Tissue Sections.- Amplification Testing in Breast Cancer by Multiplex Ligation-Dependent Probe Amplification of Microdissected Tissue.- Detection and Quantification of MicroRNAs in Laser Microdissected Formalin-Fixed Paraffin-Embedded Breast Cancer Tissues.- Laser Capture Microdissection Applications in Breast Cancer Proteomics.- Proteomic Analysis of Laser Microdissected Ovarian Cancer Tissue with SELDI-TOF MS.- LCM Assisted Biomarker Discovery from Archival Neoplastic Gastrointestinal Tissues.- Purification of Diseased Cells from Barrett’s Esophagus and Related Lesions by Laser Capture Microdissection.- Laser Microdissection of Intestinal Epithelial Cells and Downstream Analysis.- Application of Laser Microdissection and Quantitative PCR to Assess the Response of Oesophageal Cancer to Neoadjuvant Chemo-Radiotherapy.- Oligonucleotide Microarray Expression Profiling of Contrasting Invasive Phenotypes in Colorectal Cancer.- Evaluation of Gastrointestinal mtDNA Depletion in Mihondrial Neurogastrointestinal Encephalomyopathy (MNGIE).- Laser Microdissection for Gene Expression Study of Hepaellular Carcinomas Arising in Cirrhotic and Non-Cirrhotic Livers.- Laser Capture Microdissection of Pancreatic Ductal Adeno-Carcinoma Cells to AnalyzeEzH2 by Western Blot Analysis.- Laser-Capture Microdissection of Renal Tubule Cells and Linear Amplification of RNA for Microarray Profiling and Real-Time PCR.- Subcellular Renal Proximal Tubular Mihondrial Toxicity with Tenofovir Treatment.- Application of Laser Capture Microdissection to Study Renal Carcinogenesis.- Laser Capture Microdissection and Transcriptional Profiling in Archival FFPE Tissue in Prostate Cancer.- Quantitative Analysis of the Enzymes Associated with 5-Fluorouracil Metabolism in Prostate Cancer Biopsies.- Microdissection of Gonadal Tissues for Gene Expression Analyses.- Duplex Real Time PCR Assay for Quantifying Mihondrial DNA Deletions in Laser Microdissected Single Spiral Ganglion Cells.- Neuronal Type-Specific Gene Expression Profiling and Laser-Capture Microdissection.- Region-Specific In Situ Hybridization-Guided Laser Capture Microdissection on Postmortem Human Brain Tissue Coupled with Gene Expression Quantification.- UV-Laser Microdissection and mRNA Expression Analysis of Individual Neurons from Post Mortem Parkinson’s Disease Brains.- Transcriptome Profiling of Murine Spinal Neurulation Using Laser Capture Microdissection and High-Density Oligonucleotide Microarrays.- Probing the CNS Microvascular Endothelium by Immune-Guided Laser Capture Microdissection Coupled to Quantitative RT-PCR.- Laser Capture Microdissection for Factor VIII-Expressing Endothelial Cells in Cancer Tissues.- Laser Capture Microdissection and Analysis of Liver Endothelial Cells from Patients with Budd Chiari Syndrome.- Laser Capture Microdissection of Hyperlipidemic/ApoE^-/- Mouse Aorta Atherosclerosis.- Gene Expression Profiling in Laser Microdissected Bone Marrow Megakaryocytes.- Specific RNA Collection from the Rat Endolymphatic Sac by LaserCapture Microdissection (LCM): LCM of Very Small Organ Surrounded by Bony Tissue.- The Use of Laser Capture Microdissection on Adult Human Articular Cartilage for Gene Expression Analysis.- Laser Capture Microdissection of Developing Barley Seeds and cDNA Array Analysis of Selected Tissues.- Quantitative RT-PCR Gene Expression Analysis of a Laser Microdissected Placenta: An Approach to Study Preeclampsia.

Editorial Reviews

4 Stars! from Doody

Reviewer: Mirna Lechpammer, MD, PhD (Rhode Island Hospital)
Description: This book offers a comprehensive overview of current applications of laser microdissected tissue ranging from DNA and RNA analysis to applications in proteomics and molecular analysis of microorganisms.
Purpose: The book is conceptualized to provide readers with practical guidance on how to successfully use the available laser capture microdissection (LCM) systems in their own laboratories and how best to apply a wide range of molecular technologies. The editors have thus addressed a growing need for a comprehensive set of protocols, providing a valuable tool for wide range of researchers.
Audience: Due to its unique concept, covering methods applied from basic histology and cancer research to microbiology, this book is an equally useful guide for undergraduate students, postdoctoral fellows, and research scientists in academia and industry likewise.
Features: The editors have gathered an impressive international panel of contributors to cover a variety of applications of laser microdissected tissue including polymerase chain reaction (PCR) in its many different forms and gene expression analysis involving microarrays and proteomics. The individual chapters encompass detailed descriptions of several currently available LCM systems. However, the authors do not provide critical comparison of these systems, but rather provide practical guidance on each of them to meet individual needs of prospective readers. The chapters thus contain complete protocols on tissue preparation and DNA and/or RNA analysis, and are adequately illustrated with both the LCM histologic images and the results of biochemical analyses.
Assessment: Overall, this book will successfully meet the expectations of readers searching for a step-by-step laboratory cookbook and for the guidance in a growing use of the LCM technology in contemporary research.

Doody's Review Service