Liposome Methods and Protocols / Edition 1

by Subhash C. Basu, Manju Basu

Overview

In vitro utilization of liposomes is now recognized as a powerful tool in many bioscience investigations and their associated clinical studies, e.g., liposomes in drug targeting; liposomes in gene transport across plasma and nuclear membranes; liposomes in enzyme therapy in patients with genetic disorders. However, before these areas can be effectively explored, many basic areas in liposome research require elucidation, including: (a) attachment of liposomes to cell surfaces; (b) permeation of liposomes through the plasma membranes; and (c) stability of liposomes in cell or nuclear matrices. None of these areas have been exhaustively explored and liposome researchers have ample opportunities to contribute to our knowledge. The aim of Liposome Methods and Prools is to bring together a wide range of detailed laboratory prools covering different aspects of liposome biology in order to assist researchers in those rapidly advancing medical fields mentioned earlier. With this goal in mind, in each prool chapter we have detailed the materials to be used, followed by a step-by-step prool. The Notes section of each prool is also certain to prove particularly useful, since the authors include troubleshooting tips straight from their benchtops, valuable information that is seldom given in restricted methods sections of standard research journals. For this reason we feel that the book will prove especially useful for all researchers in the liposome field.

Product Details

ISBN-13:	9781617372179
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/10/2010
Series:	Methods in Molecular Biology , #199
Edition description:	Softcover reprint of hardcover 1st ed. 2002
Pages:	252
Product dimensions:	5.98(w) x 9.02(h) x 0.36(d)

Table of Contents

Physical Properties.- Preparation, Isolation, and Characterization of Liposomes Containing Natural and Synthetic Lipids.- Preparation and Use of Liposomes for the Study of Sphingolipid Segregation in Membrane Model Systems.- Liposome Fusion/Modulation.- Peptide-Induced Fusion of Liposomes.- Liposomes.- Lipids in Viral Fusion.- Application of Liposomes.- Liposome-Mediated, Fluorescence-Based Studies of Sphingolipid Metabolism in Intact Cells.- Micelles and Liposomes in Metabolic Enzyme and Glycolipid Glycosyltransferase Assays.- Liposomes and Phospholipid Binding Proteins in Glycoprotein Biosynthesis.- Therapeutic Uses of Antioxidant Liposomes.- Targeted Gene Delivery by Virosomes*.- Liposomes Containing Ligands.- Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes.- Use of Liposomes Containing Carbohydrates for Production of Monoclonal Antibodies.- Inhibition of Tumor Metastasis by Liposomes Containing Glyco-Replica Peptides.- Use of Phospholipid Bilayers and Monolayers in Binding Studies of Vitamin K-Dependent Blood Coagulation Proteins.

Editorial Reviews

"...those who deal with liposome in their work, this book with detailed laboratory prools and many notes will be very useful." -Pharmaceutical Research

"Volume 199 of the series Methods in Molecular Biology™, offers an excellent overview of prools used for the study of liposomes in biology...the book portrays comprehensive reports of the study of the cellular fate of sphingolipids using fluoresrole lipids play in virus fusion, studies of functions of various membrane-associated proteins, specifically of phospholipid binding proteins in glycoprotein biosynthesis and contributions of therapeutical liposome applications." -Molecular Biotechnology

From the Publisher

Laboratory techniques for elucidating liposome biology are described in step-by-step detail to aid researchers working in drug targeting, gene transport across plasma and nuclear membranes, and enzyme therapy for patients with genetic disorders. Each protocol is presented by an investigator familiar with its problems and capabilities, and includes a list of materials, troubleshooting tips, and pitfalls to avoid. The protocols are of interest to researchers in glycoproteins, glycolipids, antibody delivery, and protease delivery. The editors are affiliated with the Department of Chemistry and Biochemistry at the University of Notre Dame. Annotation c. Book News, Inc., Portland, OR (booknews.com)