LooseLeaf for Benson's Microbiological Applications Laboratory Manual--Complete Version / Edition 14

by Heidi Smith, Alfred E. Brown

Overview

Benson's Microbiological Applications has been the "gold standard" of microbiology laboratory manuals for over 35 years. This manual has a number of attractive features that resulted in its adoption in universities, colleges, and community colleges. These features include user-friendly diagrams that students can easily follow, clear instructions, and an excellent array of reliable exercises suitable for beginning or advanced microbiology courses. In revising the lab manual for the fourteenth edition, we have tried to maintain the proven strengths of the manual and further enhance it. We have updated the introductory material of the fungi, protozoa, and algae to reflect changes in scientific information. Finally, the names of microorganisms used by the American Type Culture Collection. This is important for those users who rely on the ATCC for a source of cultures.

Product Details

ISBN-13:	9781259919794
Publisher:	McGraw Hill LLC
Publication date:	09/19/2016
Edition description:	New Edition
Pages:	560
Product dimensions:	8.50(w) x 10.80(h) x 0.80(d)
Age Range:	18 Years

About the Author

Heidi Smith leads the microbiology discipline at Front Range Community College in Fort Collins, CO, teaching a variety of biology courses each semester including microbiology, anatomy/ physiology, and biotechnology Collaboration with other faculty across the nation, the development and implementation of new digital learning tools, and her focus on student learning outcomes have revolutionized her face-to-face and online teaching approaches and student performance in her classes. Outside of the classroom, Heidi oversees a federal grant program designed to train and support underrepresented students for undergraduate research and transfer into four-year STEM degree programs. She is also an active member of the American Society for Microbiology and participated as a task force member for the development of their Curriculum Guidelines for Undergraduate Microbiology Education. Off campus, Heidi enjoys the beautiful Colorado outdoors with her husband and three young children.

Professor and Chair, Consultant Environmental Microbiology

Table of Contents

Part 1 Microscopy1 Brightfield Microscopy2 Darkfield Microscopy3 Phase-Contrast Microscopy4 Fluorescence Microscopy5 Microscopic MeasurementsPart 2 Survey of Microorganisms6 Microbiology of Pond Water - Protists, Algae, and Cyanobacteria7 Ubiquity of Bacteria8 The Fungi: Molds and YeastsPart 3 Manipulation of Microorganisms9 Aseptic Technique10 Pure Culture TechniquesPart 4 Staining and Observations of Microorganisms11 Smear Preparation12 Simple Staining13 Negative Staining14 Capsular Staining15 Gram Staining16 Spore Staining: Two Methods17 Acid-Fast Staining: Kinyoun Method18 Motility DeterminationPart 5 Culture Methods19 Culture Media Preparation20 Preparation of Stock Cultures21 Enumeration of Bacteria: The Standard Plate Count22 Slide Culture: FungiPart 6 Bacterial Viruses23 Determination of a Bacteriophage Titer24 A One-Step Bacteriophage Growth Curve25 Isolation of Phage from Flies26 Phage TypingPart 7 Environmental Influences and Control of Microbial Growth27 Effects of Oxygen on Growth28 Temperature: Effects on Growth29 pH and Microbial Growth30 Water Activity and Osmotic Pressure31 Ultraviolet Light: Lethal Effects32 The Effects of Lysozyme on Bacterial Cells33 Evaluation of Alcohol: Its Effectiveness as an Antiseptic34 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method35 Evaluation of Antiseptics: The Filter Paper Disk Method36 Effectiveness of Hand ScrubbingPart 8 Identification of Unknown Bacteria37 Morphological Study of An Unknown Bacterium38 Cultural Characteristics39 Physiological Characteristics: Oxidation and Fermentation Stet Reactions40 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions41 Physiological Characteristics: Multiple Test Media42 Use of Bergey’s ManualPart 9 Miniaturized Multitest Systems43 Enterobacteriaceae Identification: The API 20E System44 Enterobacteriaceae Identification: Entero-Pluri Test
45 Staphylococcus Identification: The API Staph SystemPart 10 Diversity and Environmental Microbiology46 Isolation of an Antibiotic Producer: The Streptomyces47 Nitrogen Cycle: Ammonification48 Symbiotic Nitrogen Fixation: Rhizobium49 Free-Living Nitrogen Fixation: Azotobacter50 Denitrification: Paracoccus denitrificans51 The Winogradsky Column52 Purple Nonsulfur Photosynthetic Bacteria53 Sulfate Reducing Bacteria: Desulfovibrio54 Bacterial Commensalism55 Bacterial Synergism56 Microbial AntagonismPart 11 Applied Microbiology57 Bacterial Food Counts58 Bacteriological Examination of Water: Most Probable Number Determination59 Bacteriological Examination of Water: The Membrane Filter Method60 Reductase Test61 Temperature: Lethal Effects62 Microbial Spoilage of Canned Food63 Microbiology of Alcohol FermentationPart 12 Bacterial Genetics and Biotechnology64 Understanding Antibiotic Resistance: Replica Plating
65 Polymerase Chain Reaction for Amplifying DNA
66 Bacterial Transformation
Part 13 Medical Microbiology67 The Staphylococci: Isolation and Identification68 The Streptococci and Enterococci: Isolation and Identification69 Gram-Negative Intestinal Pathogens70 A Synthetic EpidemicPart 14 Immunology and Serology71 Slide Agglutination Test: Serological Typing72 Slide Agglutination Test for S. aureus73 Slide Agglutination Test for Streptococcus74 Enzyme-Linked Immunosorbent Assay (ELISA)
75 Blood GroupingAppendices