Low-Temperature Microscopy and Analysis / Edition 1

Low-Temperature Microscopy and Analysis / Edition 1

by Patrick Echlin
ISBN-10:
0306439840
ISBN-13:
9780306439841
Pub. Date:
03/31/1992
Publisher:
Springer US
ISBN-10:
0306439840
ISBN-13:
9780306439841
Pub. Date:
03/31/1992
Publisher:
Springer US
Low-Temperature Microscopy and Analysis / Edition 1

Low-Temperature Microscopy and Analysis / Edition 1

by Patrick Echlin

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Overview

The frozen-hydrated specimen is the principal element that unifies the subject of low­ temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of common fact and theory upon which their work must be based. Low-Temperature Micros­ copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro­ molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all the more necessary in electron microscopy because of the enormous increase in resolution that is available with electron-optical instruments, compared to light­ optical microscopes.

Product Details

ISBN-13: 9780306439841
Publisher: Springer US
Publication date: 03/31/1992
Edition description: 1992
Pages: 540
Product dimensions: 6.14(w) x 9.21(h) x 0.05(d)

Table of Contents

The Properties and Structure of Water. The Structure and Properties of Frozen Water and Aqueous Solutions. Sample Cooling Proceedures. Cryosectioning. Low Temperature Fracturing and Freeze Fracture Replication. Freeze Drying. Freeze Substitution and Low Temperature Embedding. Low Temperature Light Microscopy. Low Temperature Transmission Electron Microscopy. Low Temperature Scanning Electron Microscopy. Low Temperature Microanalysis. Index.
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