Macromolecular Crystallography Protocols, Volume 1: Preparation and Crystallization of Macromolecules

by Sylvie Doublie (Editor)

Overview

Macromolecular Crystallography Prools, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators.

Volume 1 is composed of detailed prools for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.

Product Details

ISBN-13:	9781617374753
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/10/2010
Series:	Methods in Molecular Biology , #363
Edition description:	Softcover reprint of hardcover 1st ed. 2007
Pages:	280
Product dimensions:	5.98(w) x 9.02(h) x 0.03(d)

Table of Contents

A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag.- Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project.- Baculoviral Expression of an Integral Membrane Protein for Structural Studies.- Protein Engineering.- Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems.- How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals.- Screening and Optimization Methods for Nonautomated Crystallization Laboratories.- Improving Marginal Crystals.- Optimization Techniques for Automation and High Throughput.- Three-Dimensional Crystallization of Membrane Proteins.- Crystallization of Protein-DNA Complexes.- Preparation and Crystallization of RNA.- Crystallization of RNA-Protein Complexes.